In vitro Anti-oxidant Activity in Methanolic Extracts of five Pogostemon Spciece

Abstract

A multifaceted peer reviewed journal in the field of Free Radicals and Antioxidants www.antiox.org | www.phcog.net Original Article INTRODUCTIONPlants have provided with all in terms of shelter, clothing, food, flavours and fragrances and medicine properties. Plants have formed the basis of complicated traditional medicine systems that have been in continuation for thousands of years to provide new remedies. The Lamiaceae contains about 236 genera and 6,900 to 7,200 species, 1 in this Pogostemon is one of the important genera and a large widespread genus having 114 species in India. Both roots and leaves are mostly used by tribal people in this genus like the fresh root or poultice of the leaves is applied on the snake bites particularly Phursa (Echis carinatus) snake bite, uterine haemorrhage, leaf extract used as an insect repellent, treatment of food poison, vomiting and stomach troubles, respiratory tract infection and pollen and nectar used as a source of Panagol honey in Maharashtra. The superoxide anion (O 2 ) hydrogen peroxide (H 2 O 2 ) and hydroxyl radical (OH) are the form of active oxygen and free radicals. These are constantly formed in human body through normal metabolic action. Hence, this leads to a variety of biochemical and physiological lesion, often consequent in metabolic destruction and cell death. 3 The synthetic antioxidants were creating the genotoxic effect 4 and other chronic disease.5 Therefore, the antioxidant properties of secondary metabolites have formed the foundation of many applications in a preservation process as pharmaceuticals, alternative medicine and natural therapies. Here the studies to explore the five Pogostemon species which have been scientific conform for their potential medicinal values. MATERIALS AND METHODS Preparation of plant extractsPogostemon vestitus, P. mollis, P. speciosus, P. wightii and P. nilagiricus were colocted belongs to the family Lamiaceae were collected from natural forest of Western Ghats of Coimbatore, Tamil Nadu. The specimen was identified by Dr. M. Murugesan, Scientist B, Botanical Survey of India, Shillong. Fresh plant materials were washed under running tap water, air-dried and powdered. About 50 g of coarsely powdered plant materials (50 g/250 ml) were extracted in a soxhlet extractor for 8-10 hrs, with methanol. The extract obtained was then concentrated using a vacuum evaporator and weighted. For stock solutions, 1 mg/ml of methanolic extract was dissolved in dimethyl sulfoxide (DMSO). Determination of Total phenolsTotal phenolics were quantified and expressed as Gallic acid equivalents according to a method proposed by Singleton et al. 7 About 3.9 ml of distilled water and 0.5 mL of Folin-ciocalteau reagent were added to 0.1ml of the extract in a tube and incubated at room temperature for 3 min after which 2 mL of 20% sodium carbonate was added and kept in a boiling water bath for 1 min. Phenols react with phosphomolybdic acid in the Folin-ciocalteau reagent in alkaline medium and produce a blue coloured complex (molybdenum blue) th...

Phytochemical assessment of Iphiona aucheri (Bioss.) Anderb. and its cytotoxic, antioxidant and antidiabetic activities

“…The results showed that the antioxidant activities of the Iphiona aucheri stem methanolic extract and its different fractions are concentration dependent. Similar results were observed in previous studies of Leucas aspera (Willd) link (Annapandian and Rajagopal 2017) and Pogostemon species (Kaliyappan et al 2017).…”