In vitro anti-oxidant activity, fluorescence quenching study and structural features of carbohydrate polymers from Phyllanthus emblica

Chatterjee, Udipta R.; Bandyopadhyay, Shruti S.; Ghosh, Debjani; Ghosal, Pradyot K.; Ray, Bimalendu

doi:10.1016/j.ijbiomac.2011.06.024

Cited by 21 publications

(21 citation statements)

References 31 publications

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“…Fruits of Amla or Indian gooseberry, the principal component of AR, are known to be very rich in anti-oxidants as revealed in several studies on different extracts of these fruits [31], [32], [33], [34], [35], [36]. However, effects of complete AR on life history traits have rarely been examined.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Effects Of Traditional Ayurvedic Formulations in Drosophila melanogaster Model Relate with Therapeutic Applications

et al. 2012

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BackgroundAyurveda represents the traditional medicine system of India. Since mechanistic details of therapy in terms of current biology are not available in Ayurvedic literature, modern scientific studies are necessary to understand its major concepts and procedures. It is necessary to examine effects of the whole Ayurvedic formulations rather than their “active” components as is done in most current studies.MethodsWe tested two different categories of formulations, a Rasayana (Amalaki Rasayana or AR, an herbal derivative) and a Bhasma (Rasa-Sindoor or RS, an organo-metallic derivative of mercury), for effects on longevity, development, fecundity, stress-tolerance, and heterogeneous nuclear ribonucleoprotein (hnRNP) levels of Drosophila melanogaster using at least 200 larvae or flies for each assay.ResultsA 0.5% (weight/volume) supplement of AR or RS affected life-history and other physiological traits in distinct ways. While the size of salivary glands, hnRNP levels in larval tissues, and thermotolerance of larvae/adult flies improved significantly following feeding either of the two formulations, the median life span and starvation resistance improved only with AR. Feeding on AR or RS supplemented food improved fecundity differently. Feeding of larvae and adults with AR increased the fecundity while the same with RS had opposite effect. On the contrary, feeding larvae on normal food and adults on AR supplement had no effect on fecundity but a comparable regime of feeding on RS-supplemented food improved fecundity. RS feeding did not cause heavy metal toxicity.ConclusionsThe present study with two Ayurvedic formulations reveals formulation-specific effects on several parameters of the fly's life, which seem to generally agree with their recommended human usages in Ayurvedic practices. Thus, Drosophila, with its very rich genetic tools and well-worked-out developmental pathways promises to be a very good model for examining the cellular and molecular bases of the effects of different Ayurvedic formulations.

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Section: Discussionmentioning

confidence: 99%

In Vivo Effects Of Traditional Ayurvedic Formulations in Drosophila melanogaster Model Relate with Therapeutic Applications

et al. 2012

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“…A number of separate spin systems attributable to anomeric protons of component sugar residues were distinguishable in the spectrum of the purified polymer. The signals appearing in the region between 5.1 and 6.1 ppm are resonances of the anomeric protons (H1) of different -linked arabinofuranosyl residues (25,26). The signals at δ H about 5.4 may be attributed to the resonance of H1 of 5-linked L-arabino- % of total area of the identified peaks; tr, trace furanosyl residues.…”

Section: Resultsmentioning

confidence: 99%

“…Indeed, a combination of cold and room temperature for the extraction of carbohydrate polymers was used because of the following reasons. First, it has been observed that the yield of the extracted carbohydrate polymers increased significantly if the plant material is stirred with water at low temperature for 12 h (25,26). Secondly, large amount of starch was extracted if the temperature of the extraction medium is being kept high for long time.…”

Section: Resultsmentioning

confidence: 99%

Carbohydrate polymers of chirata (Swertia chirata) leaves: Structural features, in vitro anti-oxidant activity and fluorescence quenching study

Ghosh

Bandyopadhyay

Chatterjee

et al. 2012

Food Sci Biotechnol

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Chirata (Swertia chirata) is widely used in Indian folk medicine for the prevention of various disorders. Herein, we analyzed the water-extracted carbohydrate polymer (WECP) of this herb using chemical, chromatographic, and spectroscopic methods. The anti-oxidant capacity of this fraction was studied by ferric reducing anti-oxidant power (FRAP) and DPPH radical assays. Effect of WECP on bovine serum albumin spectrum (BSA) was determined using excitation wavelength of 282 nm. Anion exchange chromatography of WECP yielded 5 fractions (F1-F5) with different chemical compositions. The major fraction (F5) was homogeneous, had an apparent molecular mass of 4.5 kDa, and contains both carbohydrates (57%) and phenolics (34%). The anti-oxidant capacities of WECP and F5 were comparable to standard anti-oxidants. Notably, activities of the carbohydrate polymers (F1-F5) correlate with their phenolics content. Fluorescence quenching measurement suggests that F5 can form complex with BSA and the value of the binding constant is K=6.28×10 5 /M.

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“…For the determination of neutral sugar composition, the monosaccharide residues released by acid hydrolysis were converted into their alditol acetates [17] and analyzed by gas-liquid chromatography (GLC; Shimadzu GC-17A, Shimadzu, Kyoto, Japan) and gas-liquid chromatography-mass spectrometry (GLC/MS; Shimadzu QP 5050A, Shimadzu, Kyoto, Japan). Monosaccharides were also identified by TLC [18]. Alternatively, the methyl glycosides of monosaccharides were converted into TMS-derivatives and analyzed by GLC [19].…”

Section: Saccharide Analysismentioning

confidence: 99%

“…Antioxidant assay 2.5.1. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical assay DPPH radical assay was conducted as described [18].…”

Section: Spectroscopymentioning

confidence: 99%

Structure, fluorescence quenching and antioxidant activity of a carbohydrate polymer from Eugenia jambolana

Bandyopadhyay

Ghosh

Micard

et al. 2012

International Journal of Biological Macromolecules

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In vitro anti-oxidant activity, fluorescence quenching study and structural features of carbohydrate polymers from Phyllanthus emblica

Cited by 21 publications

References 31 publications

In Vivo Effects Of Traditional Ayurvedic Formulations in Drosophila melanogaster Model Relate with Therapeutic Applications

In Vivo Effects Of Traditional Ayurvedic Formulations in Drosophila melanogaster Model Relate with Therapeutic Applications

Carbohydrate polymers of chirata (Swertia chirata) leaves: Structural features, in vitro anti-oxidant activity and fluorescence quenching study

Structure, fluorescence quenching and antioxidant activity of a carbohydrate polymer from Eugenia jambolana

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