In vitro anti-leukemic activity of the ethno-pharmacological plant Scutellaria orientalis ssp. carica endemic to western Turkey

Özmen, Ali; Madlener, Sibylle; Bauer, Sabine; Krasteva, Stanimira; Vonach, Caroline; Giessrigl, Benedikt; Gridling, Manuela; Viola, Katharina; Stark, Nicole M.; Saiko, Philipp; Michel, Barbara; Fritzer‐Szekeres, Monika; Szekeres, Thomas; Askin-Celik, Tuelay; Krenn, Liselotte; Krupitza, Georg

doi:10.1016/j.phymed.2009.06.001

Cited by 41 publications

(29 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Asterisks indicate significance compared to untreated control (p<0.05) and error bars indicate ± SD. pro-apoptotic properties of different Scrophularia species, and beyond that we show that S. lucida inhibits LEC-CCID formation by co-cultivated MCF-7 cancer cell spheroids (14,17) and inhibited NF-κB activity. Recently we were able to demonstrate that NF-κB activity contributed to LEC-CCID formation through inhibition of VE-cadherin expression and loss of intraspecific LEC adhesion (16).…”

Section: Discussionsupporting

confidence: 56%

See 1 more Smart Citation

Effects of Scrophularia extracts on tumor cell proliferation, death and intravasation through lymphoendothelial cell barriers

et al. 2012

View full text Add to dashboard Cite

Abstract. Different studies describe the anti-inflammatory effects of Scrophularia species, a medicinal plant widely used in folk medicine since ancient times. As knowledge regarding the anti-neoplastic properties of this species is rather limited, we investigated the influence of methanol extracts of different Scrophularia species on cell proliferation, cell death, and tumour cell intravasation through the lymph endothelial barrier. HL-60 leukaemia cells were treated with methanol extracts of different Scrophularia species leading to strong growth inhibition and high cell death rates. The expression of cell cycle regulators, oncogenes and cell death inducers was determined by Western blot analysis. Furthermore the effect of S. lucida was studied in an NF-κB reporter assay, and in a novel assay measuring 'circular chemo-repellent-induced defects' (CCID) in lymph endothelial monolayers that were induced by MCF-7 breast cancer spheroids. Methanol extracts of Scrophularia species exhibited strong anti-proliferative properties. S. floribunda extract inhibited G2/M-and later on S-phase and S. lucida inhibited S-phase and in both cases this was associated with the down-regulation of c-Myc expression. Extracts of S. floribunda and S. lucida led to necrosis and apoptosis, respectively. Furthermore, S. lucida, but not S. floribunda, effectively attenuated tumour cell intravasation through lymph endothelial cell monolayers, which correlated with the inhibition of NF-κB. S. lucida exhibited promising anti-neoplastic effects and this was most likely due to the downregulation of various cell cycle regulators, proto-oncogenes and NF-κB and the activation of caspase-3.

show abstract

Section: Discussionsupporting

confidence: 56%

“…For the proliferation-and apoptosis assays the following concentrations as calculated for dried plant material were used: 500 µg/ml, 1, 4 and 10 mg/ml. To exclude an effect of ethanol on cell proliferation and apoptosis, controls were treated with same concentrations of ethanol as used for sample treatment (in general 0.2% EtOH) (13,14).…”

Section: Methodsmentioning

confidence: 99%

Effects of Scrophularia extracts on tumor cell proliferation, death and intravasation through lymphoendothelial cell barriers

et al. 2012

View full text Add to dashboard Cite

show abstract

“…The methanol extract of Scutellaria orientalis showed potent anti-leukemic activity against HL-60 cell line (Ozmen et al, 2010). The water extract of Rheum officinale exhibited significant antiproliferative activity by inducing apoptosis in MCF-7 and A549 cell lines (Li et al, 2009).…”

Section: The Use Of Plants In Cancer Therapymentioning

confidence: 99%

Anticancer and Antimicrobial Potential of Plant-Derived Natural Products

Talib¹

2011

Phytochemicals - Bioactivities and Impact on Health

View full text Add to dashboard Cite

“…Hoechst 33258 propidium iodide double staining of the DNA was performed according to the method described (6). HL-60 cells (1x10 5 per ml) were seeded in T-25 Nunc tissue culture flask and exposed to increasing concentrations of plant extracts (500 μg/ml, 1 mg/ml, and 4 mg/ml; see Table I for respective extract weights) for 48 h. MCF-7 cells were seeded at a density of 1x10 4 cells per ml in 24-well plates and grown for 24 h. Then, increasing concentrations of extract were added and the cells were treated for 96 h. Then the cells were washed with PBS, treated with trypsin and resuspended with medium. Hoechst 33258 and propidium iodide were added directly to the cells at final concentrations of 5 and 2 μg/ml, respectively.…”

Section: Proliferation Inhibition Analysismentioning

confidence: 99%

“…Studies on the wild lettuce Lactuca indica analysed the effects of ethanol extracts in HL-60 cells and demonstrated that this extract type contained polyphenols that were most likely responsible for the inhibition of cell growth and the induction of apoptosis (1,2). In previous studies the extracts of traditional antiinflammatory healing plants were investigated regarding their in vitro anticancer properties (3)(4)(5) and extracts of the lettuce Lactuca sativa (iceberg) served as controls. L. sativa was sequentially extracted with solvents of increasing polarity similar to the medicinal plants investigated.…”

Section: Introductionmentioning

confidence: 99%

Anti-leukaemic effects of two extract types of Lactuca sativa correlate with the activation of Chk2, induction of p21, downregulation of cyclin D1 and acetylation of α-tubulin

Gridling

Popescu²,

Kopp³

et al. 2010

Oncol Rep

View full text Add to dashboard Cite

Abstract. The water extract of the lettuce Lactuca sativa, but not the ethyl acetate extract, inhibited the growth of HL-60 leukaemia cells and MCF-7 breast cancer cells. This correlated with the activation of checkpoint kinase 2 (Chk2), the induction of the tumour suppressor p21, and the severe downregulation of the proto-oncogene cyclin D1. The ethyl acetate extract, but not the water extract, induced HL-60 cell death, which correlated with the acetylation of ·-tubulin. The acetylation of ·-tubulin is indicative for microtubuli stabilisation such as induced by taxol. The calculated amount for human intake would require approximately 3 kg lettuce to reach the required concentration shown to inhibit 50% HL-60 proliferation.

show abstract

In vitro anti-leukemic activity of the ethno-pharmacological plant Scutellaria orientalis ssp. carica endemic to western Turkey

Cited by 41 publications

References 37 publications

Effects of Scrophularia extracts on tumor cell proliferation, death and intravasation through lymphoendothelial cell barriers

Effects of Scrophularia extracts on tumor cell proliferation, death and intravasation through lymphoendothelial cell barriers

Anticancer and Antimicrobial Potential of Plant-Derived Natural Products

Anti-leukaemic effects of two extract types of Lactuca sativa correlate with the activation of Chk2, induction of p21, downregulation of cyclin D1 and acetylation of α-tubulin

Contact Info

Product

Resources

About