In vitro anti-inflammatory activity and tissue culture studies on Tinospora cordifolia

Abstract

The present study deals with the in vitro anti-inflammatory in methanolic extracts of in vivo (leaf and stem) and in vitro (callus) plant parts of Tinospora cordifolia (Acanthaceae) is a medicinal plant of immense therapeutic value. The present work is based on developing a protocol for the callus induction in T. cordifolia from stem and leaf explants. The highest rate of callus (70.82%) was observed on MS medium fortified with 1.5 mg/L 2, 4-D and 0.3 mg/L BAP. The methanolic extracts of all plant parts exhibited remarkable anti-inflammatory activity action. The maximum amount of membrane stabilization was found in stem callus (78.15% at a dose of 100μg/ml) as compare to other plant and callus parts tested. Therefore, our studies support the isolation and the use of active constituents from in vivo and in vitro plant parts of T. cordifolia in treating inflammations.