In vitro anti inflammatory activity of Aloe vera by down regulation of MMP-9 in peripheral blood mononuclear cells

Vijayalakshmi, Damodharan; Dandapani, Ramamurthy; Jayaveni, Sivalingam; Jithendra, Panneer Selvam; Rose, C.; Mandal, Asit Baran

doi:10.1016/j.jep.2012.02.040

Cited by 80 publications

(59 citation statements)

References 17 publications

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“…Our results show that Aloe vera treatment significantly down-regulated LPS-induced IL-8, TNF␣, IL-6, and IL-1␤ inflammatory cytokine production in a concentration dependent manner in both THP-1 cells and primary macrophages. These results are in a good agreement with studies showing antiinflammatory effects of Aloe vera in animal models without molecular explanation or showing effect on IL-1␤ and TNF␣ production in non-differentiated THP-1 cells (Prabjone et al, 2006;Park et al, 2011;Yun et al, 2009;Vijayalakshmi et al, 2012;Habeeb et al, 2007;Duansak et al, 2003). It should be noted, that not only the cytotoxic sensitivity of primary macrophages was higher than that of THP-1 cells, but also the susceptibility toward Aloe-mediated down-regulation was more pronounced in these cells.…”

Section: Discussionsupporting

confidence: 89%

“…Aloe vera has recently been shown to inhibit inflammatory responses by downregulating MMP-9 in peripheral blood leukocytes (PBLs), and to reduce cytokine (TNF␣ and IL-1␤) production in undifferentiated THP-1 cells and PBLs (Vijayalakshmi et al, 2012;Habeeb et al, 2007). The anti-inflammatory effect of the supernatant of a commercial stabilized gel (from Forever Living Product) was found to have very similar effects to those found with a reconstituted lyophilized powder.…”

Section: Introductionmentioning

confidence: 90%

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Aloe vera downregulates LPS-induced inflammatory cytokine production and expression of NLRP3 inflammasome in human macrophages

Budai

Varga

Milesz³

et al. 2013

Molecular Immunology

151

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a b s t r a c tAloe vera has been used in traditional herbal medicine as an immunomodulatory agent inducing antiinflammatory effects. However, its role on the IL-1␤ inflammatory cytokine production has not been studied. IL-1␤ production is strictly regulated both at transcriptional and posttranslational levels through the activity of Nlrp3 inflammasome. In this study we aimed to determine the effect of Aloe vera on the molecular mechanisms of Nlrp3 inflammasome-mediated IL-1␤ production in LPS-activated human THP-1 cells and monocyte-derived macrophages. Our results show that Aloe vera significantly reduced IL-8, TNF␣, IL-6 and IL-1␤ cytokine production in a dose dependent manner. The inhibitory effect was substantially more pronounced in the primary cells. We found that Aloe vera inhibited the expression of pro-IL-1␤, Nlrp3, caspase-1 as well as that of the P2X7 receptor in the LPS-induced primary macrophages. Furthermore, LPS-induced activation of signaling pathways like NF-B, p38, JNK and ERK were inhibited by Aloe vera in these cells.Altogether, we show for the first time that Aloe vera-mediated strong reduction of IL-1␤ appears to be the consequence of the reduced expression of both pro-IL-1␤ as well as Nlrp3 inflammasome components via suppressing specific signal transduction pathways. Furthermore, we show that the expression of the ATP sensor P2X7 receptor is also downregulated by Aloe vera that could also contribute to the attenuated IL-1␤ cytokine secretion. These results may provide a new therapeutic approach to regulate inflammasome-mediated responses.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 90%

Aloe vera downregulates LPS-induced inflammatory cytokine production and expression of NLRP3 inflammasome in human macrophages

Budai

Varga

Milesz³

et al. 2013

Molecular Immunology

151

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“…It is revealed that it has conspicuous pharmacological activities such as antibacterial (Subramanian et al, 2006;Arunkumar and Muthuselvam, 2009;Saritha et al, 2010;Fani and Kohanteb, 2012;Nejatzadeh-Barandozi, 2013), antifungal (Bajwa et al, 2007;Rosca-Casian et al, 2007;Khaing, 2011;Sitara et al, 2011), antiviral (Zandi et al, 2007), antioxidant (Baradaran et al, 2013;Ray et al, 2013;Kang et al, 2014), cytotoxic (Jose et al, 2014;Shalabi et al, 2015), antidiabetic (Tanaka et al, 2006;Choudhary et al, 2014;Suleyman et al, 2014), anti-inflammatory (Vijayalakshmi et al, 2012;Bhattacharjee et al, 2014), antitumor (El-Shemy et al, 2010;Srihari et al, 2015), nephroprotective (Iftikhar et al, 2015;Virani et al, 2016), antiulcer (Borra et al, 2011) and anti-aging effects which can be used as a moisturizing agent to cure cardiovascular diseases as well as to enhance the immune system (Chatterjee et al, 2013). It is used as an herbal medicine since long time which contains more than 100 bioactive constituents.…”

Section: Issn: 2319-7706 Volume 6 Number 3 (2017) Pp 2152-2162mentioning

confidence: 99%

Antimicrobial Activity and Phytochemical Screening of Aloe vera (Aloe barbadensis Miller)

Dharajiya¹,

Patel²

2017

Int.J.Curr.Microbiol.App.Sci

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“…Studies performed in vitro with Aloe vera and human monocytic cell line, human blood mononuclear cells and isolated human and microbial metalloproteinases (MMP), described suppressing bacterials-induced pro-inflammatory cytokines, inhibition of collagenase and metalloproteinases, and down-regulation of MMP-9 in these cells (Barrantes and Guinea 2003, Habeeb et al 2007, Vijayalakshmi et al 2012. Elucidation of the mechanisms of Aloe gel action on donor lymphocytes will be the matter of our further study.…”

Section: Discussionmentioning

confidence: 98%

In vivo inhibitory effect of Aloe vera gel on the ability of mouse parental splenic lymphocytes to induce cutaneous angiogenesis in recipient F1 mice

Skopiński¹,

Lewicki

Bałan³

et al. 2014

Polish Journal of Veterinary Sciences

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Lymphocyte-induced angiogenesis test (LIA) is a model of local graft-versus-host (GVH) reaction, marker of the earliest events resulting from activation of donor lymphocytes after contact with host semi-allogeneic histocompatibility antigens. The effect of in vivo oral administration of Aloe vera gel for 21 days to maternal strain (Balb/c) donor mice on the ability of their splenic lymphocytes to induce cutaneous angiogenesis (LIA test) in F1 Balb/c x C3H recipients, was studied.Results: Neovascular reaction evaluated 72 hours after cells grafting was significantly lower in F1 mice grafted with lymphocytes collected from Aloe-fed donors, than in recipients of lymphocytes collected from respective controls. Conclusions: This observation opens the promise of safe and ethically acceptable possibility of use of Aloe vera gel in human donors in prevention of GVHD in recipients of bone marrow grafts.

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In vitro anti inflammatory activity of Aloe vera by down regulation of MMP-9 in peripheral blood mononuclear cells

Cited by 80 publications

References 17 publications

Aloe vera downregulates LPS-induced inflammatory cytokine production and expression of NLRP3 inflammasome in human macrophages

Aloe vera downregulates LPS-induced inflammatory cytokine production and expression of NLRP3 inflammasome in human macrophages

Antimicrobial Activity and Phytochemical Screening of Aloe vera (Aloe barbadensis Miller)

In vivo inhibitory effect of Aloe vera gel on the ability of mouse parental splenic lymphocytes to induce cutaneous angiogenesis in recipient F1 mice

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