In vitro anti-HIV activity of sulfated amphotericin B

Jc, Gluckman; Fretz-Foucault, C; Rouzioux, C.; Perret, Pascale; Lopez, Olga Cruz; Bucquet, D; Bahraoui, Elmostafa

doi:10.1097/00002030-198912000-00013

Cited by 3 publications

(2 citation statements)

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“…Finally, OTAKE et al (84) demonstrated that IP 6 inhibited the cytopathic effect of human inmunodeficiency virus (HIV) and HIV-specific antigen expression in MT-4 cells; inositol hexasulphate showing a even stronger inhibition. Coupled with the facts that IP6 enhances NK-cell activity and polymorphonuclear cell priming function, it is possible that IP 6 may have use in the management of HIV infection and associated immunodeficiency-related problems.…”

Section: Other Biological Effects and Applications Of Ip6mentioning

confidence: 99%

The role of phytic acid in legumes: antinutrient or beneficial function?

et al. 2000

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This review describes the present state of knowledge about phytic acid (phytate), which is often present in legume seeds. The antinutritional effects of phytic acid primarily relate to the strong chelating associated with its six reactive phosphate groups. Its ability to complex with proteins and particularly with minerals has been a subject of investigation from chemical and nutritional viewpoints. The hydrolysis of phytate into inositol and phosphates or phosphoric acid occurs as a result of phytase or nonenzymatic cleavage. Enzymes capable of hydrolysing phytates are widely distributed in micro-organisms, plants and animals. Phytases act in a stepwise manner to catalyse the hydrolysis of phytic acid. To reduce or eliminate the chelating ability of phytate, dephosphorylation of hexa- and penta-phosphate forms is essential since a high degree of phosphorylation is necessary to bind minerals. There are several methods of decreasing the inhibitory effect of phytic acid on mineral absorption (cooking, germination, fermentation, soaking, autolysis). Nevertheless, inositol hexaphosphate is receiving increased attention owing to its role in cancer prevention and/or therapy and its hypocholesterolaemic effect.

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Section: Other Biological Effects and Applications Of Ip6mentioning

confidence: 99%

The role of phytic acid in legumes: antinutrient or beneficial function?

et al. 2000

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“…Shortly after the identification and isolation of the virus responsible for the acquired immune deficiency syndrome (AIDS) (subsequently named the human immunodeficiency virus, HIV), several groups, following a suggestion of De Clercq (1986), demonstrated that heparin and other polysulfates are potent and selective inhibitors of HIV-1 replication in cell culture (Ito et el., 1987(Ito et el., , 1991suya et el., 1988;Mizumoto et ei., 1988;Yoshida et al, 1988;Hirabayashi et el., 1989;Jurkiewicz et el., 1989;Sugawara et al, 1989;Tochikura et al, 1989;Anand et el., 1990a,b;Kaneko et al, 1990;Montefiori et al, 1990;Weiler et al, 1990;Schols et sl., 1990aSchols et sl., , 1991Von Briesen et al, 1990;Aoki et al, 1991;Bagasra et al, 1988Bagasra et al, , 1991Sosa et al, 1991;Handa et al, 1991;Hatanaka et al, 1991;McClure et al, 1991;Moriya et al, 1991Moriya et al, , 1993Otake et al, 1991Otake et al, , 1994Witvrouw et al, 1991Witvrouw et al, , 1992Witvrouw et al, , 1994Mizuno et al, 1992;Uryu et al, 1992;Barzu et al, 1993;Beutler et al, 1993;Damonte et el., 1994). Several polysulfates were also found to be active against a wide variety of enveloped viruses (Baba et el., 1988c;De Clercq, 1990, 1993;Andrei et el., 1991;Schols et al...…”

Section: Introductionmentioning

confidence: 99%

Antiviral Portrait Series: 4. Polysuifates as Inhibitors of HIV and Other Enveloped Viruses

Witvrouw¹,

Desmyter²,

Clercq

1994

Antivir Chem Chemother

101

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Polysulfates are highly potent and selective inhibitors of the in vitro replication of HIV and other enveloped viruses. They not only inhibit the cytopathic effect of HIV, but also prevent HIV-induced syncytium (giant cell) formation. They also act synergistically with other anti-HIV drugs. The anti-HIV activity of polysulfates is a result of their shielding of the positively charged sites in the V3 loop of the viral envelope glycoprotein gp120. When polysulfates were administered intravenously to rabbits, their half-life was approximately 2h. Although they are very poorly absorbed following oral administration, they can be made orally bioavailable with the appropriate chemical modifications. Also, polysulfates may lose (much of) their anticoagulant activity upon chemical modification without giving up their anti-HIV activity. Their efficacy in the therapy and/or prophylaxis of retroviral infections remains to be demonstrated both in animal models and in humans.

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Amphotericin-B-mediated reactivation of latent HIV-1 infection

Jones¹,

Kosloff²,

Benveniste³

et al. 2005

Virology

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To date, attempts to eliminate HIV-1 infection from its latent reservoirs, a prerequisite for the development of a curative treatment strategy for HIV-1 infection, have been unsuccessful. We demonstrate that the FDA approved antifungal agent amphotericin B efficiently reactivates HIV-1 infection in THP89GFP cells, a model of HIV-1 latency in macrophages. Although amphotericin B does not directly reactivate latent HIV-1 infection in T cells (e.g., J89GFP), amphotericin-B-stimulated macrophages (THP89GFP cells or primary macrophages) when cocultured with J89GFP cells can induce HIV-1 reactivation in these cells in trans. Because of the close proximity of antigen presenting macrophages and T cells in the primary lymphoid organs, such interaction between antigen presenting macrophages and T cells are frequent, and it seems reasonable to assume that trans-reactivation strategies hold promise to also reactivate latent HIV-1 infection in vivo.

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In vitro anti-HIV activity of sulfated amphotericin B

Cited by 3 publications

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The role of phytic acid in legumes: antinutrient or beneficial function?

The role of phytic acid in legumes: antinutrient or beneficial function?

Antiviral Portrait Series: 4. Polysuifates as Inhibitors of HIV and Other Enveloped Viruses

Amphotericin-B-mediated reactivation of latent HIV-1 infection

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