In vitro anti-Helicobacter pylori activity of Syzygium aromaticum and the preliminary mechanism of action

Chang, Peng; Sang, Shuyi; Shen, Xue; Zhang, Weijia; Yan, Jiahui; Chen, Pengting; Jiang, Cheng; Yuan, Yuemei; Zhu, Weichun; Yao, Meicun

doi:10.1016/j.jep.2022.114995

Cited by 20 publications

(18 citation statements)

References 77 publications

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“…Several natural products with anti- H. pylori properties are found in the scientific literature, such as Aloe vera [ 12 ], Syzygium aromaticum [ 13 ], Canarium album Raeusch. [ 14 ], essential oils and South African honey [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Brazilian Red Propolis Presents Promising Anti-H. pylori Activity in In Vitro and In Vivo Assays with the Ability to Modulate the Immune Response

et al. 2022

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Helicobacter pylori is a Gram-negative, microaerophilic, curved-rod, flagellated bacterium commonly found in the stomach mucosa and associated with different gastrointestinal diseases. With high levels of prevalence worldwide, it has developed resistance to the antibiotics used in its therapy. Brazilian red propolis has been studied due to its biological properties, and in the literature, it has shown promising antibacterial activities. The aim of this study was to evaluate anti-H. pylori from the crude hydroalcoholic extract of Brazilian red propolis (CHEBRP). For this, in vitro determination of the minimum inhibitory and bactericidal concentration (MIC/MBC) and synergistic activity and in vivo, microbiological, and histopathological analyses using Wistar rats were carried out using CHEBRP against H. pylori strains (ATCC 46523 and clinical isolate). CHEBRP presented MIC/MBC of 50 and 100 μg/mL against H. pylori strains (ATCC 43526 and clinical isolate, respectively) and tetracycline MIC/MBC of 0.74 µg/mL. The association of CHEBRP with tetracycline had an indifferent effect. In the stomach mucosa of rats, all treatments performed significantly decreased the number of H. pylori, and a concentration of 300 mg/kg was able to modulate the inflammatory response in the tissue. Therefore, CHEBRP showed promising anti-H. pylori in in vitro and in vivo assays.

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Section: Introductionmentioning

confidence: 99%

Brazilian Red Propolis Presents Promising Anti-H. pylori Activity in In Vitro and In Vivo Assays with the Ability to Modulate the Immune Response

et al. 2022

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“…All clinical strains were identified by the providers via morphological observation, Gram staining, and biochemical reactions. They were then all preserved at -80°C in a solution that contained identical amounts of 65% BHI, 25% glycerol, and 10% FBS [ 22 ]. For liquid incubation, bacteria were inoculated in BHI broth supplemented with 10% FBS and shaken at 150 rpm under the same air conditions as stated before [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

In vitro anti-Helicobacter pylori activity and antivirulence activity of cetylpyridinium chloride

Xun,

Feng,

et al. 2024

PLoS ONE

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The primary treatment method for eradicating Helicobacter pylori (H. pylori) infection involves the use of antibiotic-based therapies. Due to the growing antibiotic resistance of H. pylori, there has been a surge of interest in exploring alternative therapies. Cetylpyridinium chloride (CPC) is a water-soluble and nonvolatile quaternary ammonium compound with exceptional broad-spectrum antibacterial properties. To date, there is no documented or described specific antibacterial action of CPC against H. pylori. Therefore, this study aimed to explore the in vitro activity of CPC against H. pylori and its potential antibacterial mechanism. CPC exhibited significant in vitro activity against H. pylori, with MICs ranging from 0.16 to 0.62 μg/mL and MBCs ranging from 0.31 to 1.24 μg/mL. CPC could result in morphological and physiological modifications in H. pylori, leading to the suppression of virulence and adherence genes expression, including flaA, flaB, babB, alpA, alpB, ureE, and ureF, and inhibition of urease activity. CPC has demonstrated in vitro activity against H. pylori by inhibiting its growth, inducing damage to the bacterial structure, reducing virulence and adherence factors expression, and inhibiting urease activity.

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“…The inhibition and killing kinetics assays were performed as previously reported ( Shen et al, 2021 ; Peng et al, 2022 ). For the inhibition kinetics assay, H. pylori 26,695–16R cultured for 48–72 h was collected at 0 (control), 0.25, 0.5, and 1 times the MIC concentration for JWC in Brucella Broth (BD, Franklin Lakes, NJ, United States) containing 10% Foetal Bovine Serum (FBS, BI, Kibbutz Beit Haemek, Israel) and shaken (100–120 rpm) at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Revealing the novel effect of Jinghua Weikang capsule against the antibiotic resistance of Helicobacter pylori

et al. 2022

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BackgroundHelicobacter pylori (H. pylori) infects half of the human population globally. Eradication rates with triple or quadruple therapy have decreased owing to the increasing rate of antibiotic resistance. Jinghua Weikang capsule (JWC) is the first and most popular Chinese patent medicine approved by the state for the treatment of gastritis and peptic ulcers caused by H. pylori infection in China. Previous studies have found that JWC has a certain bactericidal effect on drug-resistant H. pylori and its major component, Chenopodium ambrosioides L. inhibits biofilm formation, but the mechanism remains unclear. This study focused on drug-resistant H. pylori and explored whether JWC could reverse drug resistance and its related mechanisms.MethodThe agar plate dilution method, E-test method, and killing kinetics assay were used to evaluate the bactericidal effect of JWC on antibiotic-resistant H. pylori and its effect on antibiotic resistance. Sanger sequencing was used to detect mutations in drug resistance genes. The crystal violet method, scanning electron microscopy, and confocal laser scanning microscopy were used to evaluate the effects of JWC on biofilms. qPCR was performed to evaluate the effect of JWC on the expression of efflux pump-related genes. qPCR and immunofluorescence were used to evaluate the effects of JWC on H. pylori adhesion.ResultsJWC showed considerable antibacterial activity against drug-resistant H. pylori strains, with minimum inhibitory concentration (MIC) values ranging from 64 to 1,024 μg/ml. The MIC of metronidazole (MTZ) against H. pylori 26,695–16R decreased from 64 to 6 μg/ml after treatment with 1/2 MIC of JWC. The resistance of H. pylori 26,695–16R to MTZ was reversed by JWC, and its effect was better than that of PaβN and CCCP. H. pylori 26,695–16R is a moderate biofilm-forming strain, and JWC (16–64 μg/ml) can inhibit the formation of biofilms in H. pylori 26,695–16R. JWC reduced the expression of HP0605-HP0607 (hefABC), HP0971-HP0969 (hefDEF), HP1327-HP1329 (hefGHI), and HP1489-HP1487. JWC reduced the adhesion of H. pylori to GES-1 cells and the expression of adhesives NapA, SabA, and BabA.ConclusionThe reversal of MTZ resistance by JWC may be achieved through the adhesin/efflux pump-biofilm pathway.

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In vitro anti-Helicobacter pylori activity of Syzygium aromaticum and the preliminary mechanism of action

Cited by 20 publications

References 77 publications

Brazilian Red Propolis Presents Promising Anti-H. pylori Activity in In Vitro and In Vivo Assays with the Ability to Modulate the Immune Response

Brazilian Red Propolis Presents Promising Anti-H. pylori Activity in In Vitro and In Vivo Assays with the Ability to Modulate the Immune Response

In vitro anti-Helicobacter pylori activity and antivirulence activity of cetylpyridinium chloride

Revealing the novel effect of Jinghua Weikang capsule against the antibiotic resistance of Helicobacter pylori

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