IN VITRO ANTHELMINTIC EFFECT OF <i>ANOGEISSUS LEIOCARPUS</i> (DC.) GUILL. & PERR. LEAF EXTRACTS AND FRACTIONS ON DEVELOPMENTAL STAGES OF <i>HAEMONCHUS CONTORTUS</i>

Abstract

The anthelmintic effect of acetone leaf extract and fractions of Anogeissus leiocarpus was investigated to determine the relative efficacy of the components as anthelmintic against Haemonchus contortus (Rudolphi). The fractions were obtained by solvent-solvent group separation of the leaf extract. The fractions were evaluated for ovicidal and larvicidal activity by egg hatch inhibition assay and larval development viability assay. Best-fit LC 50 values for egg hatch test were 0.360, 0.316, 0.093, 0.219 and 0.196 mg/ml for the crude acetone extract, hexane, chloroform, butanol, and 35% water in methanol fractions, respectively. While the best-fit LC 50 values for larval development and viability test were 0.509, 0.162, 0.186, 0.288 and 0.130 mg/ml for the crude acetone extract, hexane, chloroform, butanol, and 35% water in methanol fractions, respectively. The 35% water in methanol fractions was the more active on larvae, although differences in activity between fractions were not significant (p>0.05). A. leiocarpus leaf extracts could find application in anthelmintic therapy in veterinary practice.

Early Germination and Growth Trial of &lt;i&gt;Anogeissus leiocarpus &lt;/i&gt;Seeds Sourced from Three Locations in Nigeria

“…The plant has been reported to be used traditionally for the treatment of different ailments including the treatment of diabetic ulcers, general body pain, blood clots, asthma, coughing, jaundice, pile and tuberculosis [10,4,11], Malaria, Trypanosomiasis, Helminthiasis and dysenteric syndrome [12] and also, it is used against stomach infections and fungal infections such as dermatitis and Mycosis [13]. Recent studies have revealed that the plant exhibits a variety of pharmacological activities including Antiplasmodial [14], Antioxidant and hepatoprotective [15,16] Leishmanicidal [17] Anthelmintic [18] Trypanocidal [17,19] antimicrobial [20,21]. The bark and seed of the tree is used for the treatment and prevention of worm infestation in equine species.…”