In vitro and in vivo analysis of the anti-plasmodial activity of ethanol extract of Phyllanthus nivosus W. Bull leaf

Johnson, Titilayo Omolara; Istifanus, G; Kutshik, Richard Joseph

doi:10.1007/s12639-019-01178-4

Cited by 6 publications

(4 citation statements)

References 17 publications

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“…GC-MS analysis of the ethyl acetate fraction was performed using Shimadzu GC-MS -QP2010 PLUS as previously described [18]. The equipment was set at the following conditions: Injector temperature -250 C, oven temperature -60 C, ion source temperature -200 C, interface temperature -250 C, pressure -100.2 KPa, column flow -1.61 ml/min, purge flow 5.6 ml/min and total flow time -39.4 ml/min.…”

Section: Gas Chromatography-mass Spectrometry (Gc-ms) Analysismentioning

confidence: 99%

“…Amadi and colleagues (2017) reported the activity of aqueous and ethanol extracts of the leaf against Streptococcus mutans isolated from dental caries patients [16]. Onyegbule and colleagues (2014) reported the analgesic, anti-inflammatory and antimicrobial activity of the plant extracts [17]; and Johnson and colleagues (2020) reported its antimalarial activity [18]. This study aimed to evaluate the anti-inflammatory activity of various fractions of Phyllanthus nivosus in a rat model of ulcerative colitis and to identify the active ingredients.…”

Section: Introductionmentioning

confidence: 99%

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Biochemical evaluation and molecular docking assessment of the anti-inflammatory potential of Phyllanthus nivosus leaf against ulcerative colitis

Johnson

Odoh

Nwonuma

et al. 2020

Heliyon

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Ulcerative colitis (UC) is an inflammation of the colon that can progress to colorectal cancer if left untreated. No medication completely cures UC and natural products are sources of alternative approaches. This study aimed to determine the anti-inflammatory potential of Phyllanthus nivosus leaf extract and fractions in a rat model of ulcerative colitis and to identify the active ingredients. UC was induced in rats by intra-rectal infusion of 1ml of 4% acetic acid (AA) in normal saline. AA exposed groups of rats were treated with 100 mg/kg bodyweight of methanol extract, hexane, ethyl-acetate and butanol fractions orally for four days. Another group received the standard drug -Dexamethasone and control rats were given distilled water only. Some biochemical changes were evaluated and the active ingredients were identified using Gas Chromatography-Mass Spectrometry (GC-MS) followed by molecular docking against interleukin-1-beta converting enzyme (Caspase-1), beta-2 adrenergic receptor (ADRB2), cyclooxygenase-2 (COX-2) and tumour necrosis factor-alpha (TNF-α). Exposure of rat colon to acetic acid significantly altered (p < 0.05) serum levels of tumour necrosis factor-alpha (TNF-α), interleukin -6 (IL-6), nitric oxide (NO), lipid peroxidation product (malondialdehyde or MDA), reduced glutathione (GSH); and activities of superoxide dismutase (SOD) and catalase (CAT). These alterations were however restored in the rats treated with P. nivosus leaf with the ethyl-acetate fraction displaying the highest ameliorative activity. GC-MS analysis of the ethyl acetate fraction revealed the presence of 40 compounds which when subjected to molecular docking demonstrated varying degrees of binding affinities for the protein targets. Ethyl iso-allocholate demonstrated the highest binding affinity for caspase-1, cholest-22-ene-21-ol, 3,5-dehydro-6-methoxy-, pivalate for ADRB2 and TNF-α; and alpha-cadinol for COX-2. The anti-inflammatory potential of Phyllanthus nivosus leaf as a natural remedy and as a source of new drugs against ulcerative colitis is validated.

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Section: Gas Chromatography-mass Spectrometry (Gc-ms) Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biochemical evaluation and molecular docking assessment of the anti-inflammatory potential of Phyllanthus nivosus leaf against ulcerative colitis

Johnson

Odoh

Nwonuma

et al. 2020

Heliyon

Self Cite

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“…20 Hence, medicinal plants have been a major source of drugs for the treatment of a variety of diseases. 21,22 To serve as a guide, for its use in traditional preparations and as a source of new drugs, the safety profile of Tapinanthus cordifolius extract was evaluated. © 2023 the authors.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of The Toxic Effects of Ethanolic Leaf Extract of Tapinanthus cordifolius in Mice and Rats

2023

TJNPR

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IntroductionTapinanthus cordifolius is an African mistletoe in the Loranthaceae family and the Tapinanthus genus. 1,2 Mistletoe refers to woody shoot parasites found in a variety of plant groups, including the Loranthaceae and Viscaceae families. 1 The Hausa, Yoruba, and Ibo speakers in Nigeria refer to it as "kaucì", "ewe afomo," and "ohumagana" respectively. 1 It is an obligatory hemi-parasitic evergreen tropical plant that grows on a wide range of trees. 1 In West Africa, mistletoe can be found on a variety of indigenous trees as well as several economically important tree crops, such as shea butter, neem, cocoa, and rubber. Mistletoe is prevalent in South-western Nigeria, especially on tree crops such as cocoa, kola, coffee, bush mango, and others. Mistletoe can also be found on citrus trees such as oranges and guava trees. It grows as a parasitic plant on the branches and trunks of trees, sending out haustoria that pierce the tree to absorb nutrients. Mistletoe, like other plants, can also grow on its own and make its sustenance through photosynthesis. 3 Mistletoe is commonly utilized in practically every culture to treat various conditions such as hypertension, cancer, and diabetes, or as a diuretic agent. 1,2 In Nigeria, mistletoe is used to treat a variety of human and animal problems, including stomach aches, diarrhoea, dysentery, wounds, and cancer. Ruminants and local fowls enjoy it with no documented digestive problems. 3,4

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“…Malaria remains a public health concern in lower and middle-income Sub-Saharan countries in Latin America, Asia and Africa. 1,2 The earlier gains recorded in the global malaria fight between 2000 and 2013 has stalled in recent years [3][4][5][6] and the available data demonstrate a consistently disturbing increase in the number of malaria cases. There were 219 million recorded cases of malaria in 2017, which is 2 million more than in 2016.…”

Section: Introductionmentioning

confidence: 99%

High-Throughput Virtual Screening with Molecular Docking, Pharmacophore Modelling and ADME Prediction to Discover Potential Inhibitors of Plasmodium falciparum Lactate Dehydrogenase (PfLDH) from Compounds of Combretaceae Family

2021

TJNPR

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The increased prevalence of malaria requires continuous efforts towards the discovery of natural antimalarial agents targeting important biochemical pathways of the parasite. The Plasmoduim falciparum lactate dehydrogenase (pfLDH) is a glycolytic enzyme whose critical roles and unique characteristics make it an efficient antimalarial target. The aim of this study was to employ in silico methods to identify potential inhibitors of pfLDH from the selected bioactive compounds of Combretaceae species. One hundred and fifty (150) Combretaceae compounds were screened using molecular docking analysis on Schrödinger Maestro 12.5, followed by pharmacophore modelling and ADMET (absorption, distribution, metabolism, excretion and toxicity) study of the highest affinity compounds. Myricetin 3-O-glucoside and 2''-O-Galloylisovitexin showed higher binding affinities (-13.413 Kcal/mol and -12.896 Kcal/mol respectively) for pfLDH compared with -10.400 Kcal/mol displayed by nicotinamide adenine dinucleotide (NADH) (the co-factor). They interacted with GLY27, GLY29, MET30, ILE31, ASP53, GLY99, THR101 and TYR247 at the NADH binding site of the enzyme. The pharmacophore modelling showed the involvement of aromatic rings and hydrogen bond donors and acceptors in the interactions of the compounds with the target. Hence, these compounds could be said to possess the structural features, binding affinities and molecular interactions required as inhibitors of pfLDH and could be developed into antimalarial drugs following lead optimisation and experimental studies.

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In vitro and in vivo analysis of the anti-plasmodial activity of ethanol extract of Phyllanthus nivosus W. Bull leaf

Cited by 6 publications

References 17 publications

Biochemical evaluation and molecular docking assessment of the anti-inflammatory potential of Phyllanthus nivosus leaf against ulcerative colitis

Biochemical evaluation and molecular docking assessment of the anti-inflammatory potential of Phyllanthus nivosus leaf against ulcerative colitis

Evaluation of The Toxic Effects of Ethanolic Leaf Extract of Tapinanthus cordifolius in Mice and Rats

High-Throughput Virtual Screening with Molecular Docking, Pharmacophore Modelling and ADME Prediction to Discover Potential Inhibitors of Plasmodium falciparum Lactate Dehydrogenase (PfLDH) from Compounds of Combretaceae Family

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