In vitro and in vivo anthelmintic activity of Euphorbia helioscopia L.

Lone, Bashir A.; Chishti, M. Z.; Bhat, Fayaz Ahmad; Tak, Hidayatullah; Bandh, Suhaib A.

doi:10.1016/j.vetpar.2012.04.023

Cited by 28 publications

(22 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Regarding the ethanol extracts tested, there is no significant difference between the concentrations investigated (0.1 mg/mL, 1 mg/mL, and 10 mg/mL), unlike that found by Lone et al (9) in their in vitro study with extracts from Euphorbia heliscopia on H. contortus, were the extract concentration was more effective in inhibiting parasite motility as it was increased, 12.5, 25, and 50 mg/mL.…”

Section: Discussioncontrasting

confidence: 63%

“…An in vitro study, conducted by Macedo et al (8) with the essential oil of Eucalyptus globulus L. on hatching and development of Haemonchus contortus larvae, a gastrointestinal nematode of ruminants, showed ovicidal and/or larvicidal activity and potential for use in its control. In another study, methanol extracts obtained from Euphorbia helioscopia, showed anthelmintic activity when tested in vitro and in vivo on H. contortus, suggesting a treatment alternative in cases of infections due to helminths in ruminants (9).…”

Section: Introductionmentioning

confidence: 97%

“…In this regard, various studies employing medicinal plants and their derivatives have shown ovicidal and larvicidal activities against several parasites (8)(9)(10)(11)(12)). An in vitro study, conducted by Macedo et al (8) with the essential oil of Eucalyptus globulus L. on hatching and development of Haemonchus contortus larvae, a gastrointestinal nematode of ruminants, showed ovicidal and/or larvicidal activity and potential for use in its control.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Activity of Euterpe edulis Martius, Mikania glomerata Spreng, and Mikania laevigata Schultz Bip. Extracts on Gastrointestinal Nematodes Toxocara canis and Ancylostoma caninum

Zamprogno

Lopes

Lacerda

et al. 2015

Arch Clin Infect Dis

View full text Add to dashboard Cite

Background:Gastrointestinal parasitoses have high rates of morbidity and mortality. Each year about 3.5 billion people are affected by these diseases and 65,000 of them die, mostly in developing countries due to lack of basic sanitation, malnutrition, and poor access to medication. Thus, they constitute an important public health problem due to causing direct health problems related to lack of piped water, absence of sewage system, and lack of orientation. Objectives: Two in vitro assays were performed to evaluate the larvicidal and/or ovicidal activity of ethanol extracts obtained from the plants Euterpe edulis, Mikania laevigata, and Mikania glomerata on the gastrointestinal nematodes Toxocara canis and Ancylostoma caninum. Materials and Methods: In the first assay (A), T. canis eggs were exposed to three different concentrations (0.1 mg/mL, 1 mg/mL, and 10 mg/mL) of each extract, three different concentrations of albendazole (positive control), ethanol (solvent), and a negative control (no treatment), for 15 days at 26°C, under the shelter of light in order to evaluate the percentage of embryonated eggs in the presence of these extracts. In the second assay (B), the larvicidal activities of the species studied were evaluated in the different extract concentrations (0.1 mg/mL, 1 mg/mL, and 10 mg/mL), control, and solvent (ethanol), in coprocultures positive for A. caninum eggs. Results: In assay A, the results demonstrated inhibitory embryogenesis activity on T. canis eggs; however, no difference (P > 0.01) was found between the activities of the extracts. In the control group, there was a difference (P < 0.01) in relation to the tested extracts, in which this difference was not concentration-dependent. In assay B, all extracts showed inhibitory (P > 0.01) hatchability activity of A. caninum eggs in the control group. Conclusions: Through these results, the applicability of the used extracts in the control of eggs and/or larvae of T. canis and A. caninum is suggested. However, it is worth mentioning that further studies should be performed with the species E. edulis, M. glomerata and M. laevigata, using different extracts, new concentrations, and in vivo studies, in order to ensure further clarification on the agents responsible for the observed effects, degree of efficacy, and toxicity.

show abstract

Section: Discussioncontrasting

confidence: 63%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Activity of Euterpe edulis Martius, Mikania glomerata Spreng, and Mikania laevigata Schultz Bip. Extracts on Gastrointestinal Nematodes Toxocara canis and Ancylostoma caninum

Zamprogno

Lopes

Lacerda

et al. 2015

Arch Clin Infect Dis

View full text Add to dashboard Cite

show abstract

“…Porém, estudos apontam a eficácia anti-helmíntica de plantas da mesma família. No Brasil, Carvalho et al (2012), constataram a eficácia in vitro de Hura crepitans na inibição do desenvolvimento larval de Haemonchus contortus em ovinos e Lone et al (2012), in vivo com Euphorbia helioscopia.…”

Section: Eficácia Do Experimento In Vivounclassified

Estudo da atividade anti-helmíntica do extrato etanólico de Jatropha mollissima (Pohl) Baill. (Euphorbiaceae) sob Haemonchus contortus em ovinos no semiárido paraibano

et al. 2014

View full text Add to dashboard Cite

1051 tigada a concentração de extrato com efeito bioativo, pelo teste de evolução da toxicidade do extrato etanólico de J. mollissima sobre o microcrustáceo Artemia salina, obtendo uma CL50 de 660,80µg/ml, que foi testada em coproculturas contendo larvas infectantes de Haemonchus contortus e em animais para a verificação da redução do OPG. Para o teste in vivo o extrato foi dissolvido em água para se obter as concentrações 660,80µg/ml e 1321,6µg/ml, foram coletadas fezes semanalmente e sangue quinzenalmente. This study aimed to evaluate the anthelmintic effect of Jatropha mollissima through in vitro and in vivo experiments. Initially we investigated the concentration of extract with bioactive effect, through the toxicity evolution test of the ethanol extract of J. mollissima on the microcrustacean Artemia salina, obtaining CL50 concentration of 660.80µg/ml, which was tested in fecal cultures containing infective larvae of Haemonchus contortus and in animals for the verification of OPG reduction. For in vivo test, the extract was dissolved in water to obtain concentrations of 660.80µg/ml and 1321.6µg/ml. Feces were collected weekly and blood was collected every fifteen days. As a result of in vitro test, the ethanol extract of the stem of J. mollissima proved toxic on A. salina, with CL50 less than 1000µg/ ml and inhibited the eggs hatching and the development of larvae of H. contortus, presenting an efficiency of 70.77%. In vivo test revealed that the extract is also effective in sheep, with a significant reduction in the count of OPG after 28 days of experiment, 47 and 44% of reduction in the groups treated with the extract, 7.5% in the untreated group of animals and 40.6% with ivermectin. Even parasitized, the animals remained clinically healthy and without anemia. The ethanol extract of the stem of Jatropha mollissima may represent an alternative to the control of sheep worms, because it slows the parasitic resistance.

show abstract

“…In vivo: Fecal egg count reduction test and post-mortem examination are the two tests to perform in vivo anthelmintic assessment trials. Former test is adopted most due to ease of conduction and being inexpensive (Lone et al, 2012(Lone et al, , 2013Mondal et al, 2015;Grzybek et al, 2016). Fecal egg count reduction test: Efficacy is measured through reduction of number of eggs in fecal samples posttreatment with candidate drug Meenakshisundaram et al, 2016).…”

Section: Adult Motility Assaymentioning

confidence: 99%

An Overview of Plants with Acaricidal and Anthelmintic Properties

Zaman¹

2017

IJAB

View full text Add to dashboard Cite

Geo-climatic and socio-economic conditions provide a favourable environment for parasitic population of livestock in Pakistan. Hard ticks (Ixodidae) and gastrointestinal nematodes pose most serious threats to livestock industry. Stackholders rely on synthetic drugs to control these parasites. Emergence of drug resistance in these parasites; however, has provoked interest in alternate of synthetic drugs. Testing of plants used in ethnoveterinary medicine for their antiparasitic activity employing standard procedures has been reported to be promising. Plants have been most frequently used for deworming purposes followed by as acaricides and insecticides; whereas, reports as to their antiprotozoal use are relatively less. Use of plants as antiparasitics is more frequent in developing countries having low accessability to the modern parasite control practices. Likewise, validation studies on the use of plants as antiparasitics have been more frequently carried out in the developing countries compared with those having livestock farming as commercial enterprise. This article presents an overview of the plants having acaricidal and anthelmintic activity, limitations as to the application of phytotherpay to control parasites and future prospects of the use of plants as antiparasitics.

show abstract

In vitro and in vivo anthelmintic activity of Euphorbia helioscopia L.

Cited by 28 publications

References 20 publications

Activity of Euterpe edulis Martius, Mikania glomerata Spreng, and Mikania laevigata Schultz Bip. Extracts on Gastrointestinal Nematodes Toxocara canis and Ancylostoma caninum

Activity of Euterpe edulis Martius, Mikania glomerata Spreng, and Mikania laevigata Schultz Bip. Extracts on Gastrointestinal Nematodes Toxocara canis and Ancylostoma caninum

Estudo da atividade anti-helmíntica do extrato etanólico de Jatropha mollissima (Pohl) Baill. (Euphorbiaceae) sob Haemonchus contortus em ovinos no semiárido paraibano

An Overview of Plants with Acaricidal and Anthelmintic Properties

Contact Info

Product

Resources

About