In vitro and in vivo inhibitory effect of anthocyanin-rich bilberry extract on α-glucosidase and α-amylase

Ji, Yanglin; Liu, Dong; Jin, Yan; Zhao, Juan; Zhao, Jingjing; Li, Heyu; Li, Liwei; Zhang, Hua; Wang, Hao

doi:10.1016/j.lwt.2021.111484

Cited by 54 publications

(31 citation statements)

References 45 publications

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“…Alpha-glucosidase is an intestinal brush border enzyme that plays a role in the hydrolysis of oligosaccharides into glucose and the breakdown of sucrose into glucose and fructose ( Lankatillake et al, 2019 ; Ji et al, 2021 ). By delaying carbohydrate digestion and reducing the absorption of monosaccharides, the inhibitor can play a vital role in suppressing the postprandial blood glucose levels of diabetic patients and maintaining normal blood glucose levels ( Lankatillake et al, 2019 ; Zhang et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

Bioreactor-grown exo- and endo-β-glucan from Malaysian Ganoderma lucidum: An in vitro and in vivo study for potential antidiabetic treatment

Abdullah

Nasir

Azizan

et al. 2022

Front. Bioeng. Biotechnol.

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This study aims to identify the roles of exo-β-glucan (EPS-BG) and endo-β-glucan (ENS-BG) extracted from Ganoderma lucidum (GL) in inhibiting the alpha-glucosidase enzyme, a target mechanism for postprandial hyperglycaemia regulation. Upscale production of GL was carried out using a 10 L bioreactor. The zebrafish embryo toxicity test (ZFET) was carried out based on OECD guidelines. The hatching rate, survival rate, heart rate, morphological malformation, and teratogenic defects were observed and determined every 24 h from 0–120 h of post-exposure (hpe). For diabetes induction, adult zebrafish (3–4 months of age) were overfed and induced with three doses of 350 mg/kg streptozotocin (STZ) by intraperitoneal injection (IP) on three different days (days 1, 3, and 5). The oral sucrose tolerance test (OSTT) and anti-diabetic activity of EPS-BG and ENS-BG were evaluated (day 7) using the developed model (n = 15). This study showed that EPS is the most potent compound with the highest inhibitory effect toward the alpha-glucosidase enzyme with an IC50 value of 0.1575 mg/ml compared to ENS extracts (IC50 = 0.3479 mg/ml). Both EPS-BG and ENS-BG demonstrated a strong inhibition of alpha-glucosidase activity similar to the clinically approved alpha-glucosidase inhibitor, acarbose (IC50 = 0.8107 mg/ml). ENS-BG is non-toxic toward zebrafish embryos with LC50 of 0.92 mg/ml and showed no significant changes in ZE hatching and normal heart rate as compared to untreated embryos (161 beats/min). Teratogenic effects of ENS-BG (<1.0 mg/ml) on zebrafish embryonic development were not observed. The DM model of zebrafish was acquired after the third dose of STZ with a fasting BGL of 8.98 ± 0.28 mmol/L compared to the normal healthy group (4.23 ± 0.62 mmol/L). The BGL of DM zebrafish after 30 min treated with EPS-BG and ENS-BG showed a significant reduction (p < 0.0001). Both EPS-BG and ENS-BG significantly reduced DM zebrafish’s peak blood glucose and the area under the curve (AUC) in OSTT. Hence, EPS-BG and ENS-BG extracted from GL showed promising inhibition of the alpha-glucosidase enzyme and are considered non-toxic in ZE. Moreover, EPS-BG and ENS-BG reduced blood glucose levels and inhibited hyperglycemia in DM zebrafish.

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Section: Resultsmentioning

confidence: 99%

Bioreactor-grown exo- and endo-β-glucan from Malaysian Ganoderma lucidum: An in vitro and in vivo study for potential antidiabetic treatment

Abdullah

Nasir

Azizan

et al. 2022

Front. Bioeng. Biotechnol.

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“…Moreover, free radicals readily react with highly susceptible amino acid residues, and the formation of protein carbonyls can lead to muscle protein oxidative damage [51]. Remarkably, Ji et al [52] found electron donors and indicated hydrogen-bond interactions between ligands and amino acid main chains or side chains in the two-dimensional structure of anthocyanin-rich bilberry extract. Indeed, anthocyanin-rich plants might have high levels of crude protein and high amino acid concentrations [53,54].…”

Section: Discussionmentioning

confidence: 99%

The Effects of Purple Corn Pigment on Growth Performance, Blood Biochemical Indices, Meat Quality, Muscle Amino Acids, and Fatty Acids of Growing Chickens

Luo

et al. 2022

Foods

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This study investigated the effects of dietary supplementation with different levels of purple corn pigment (PCP) on the growth performance, blood biochemical indices, meat quality, muscle amino acids, and fatty acids of growing chickens. A total of 288 (8 weeks of age) growing Chishui black-bone chickens (body weight, 940 ± 80 g; mean ± standard deviation) were randomly divided into 4 groups using a completely randomized design. The four diet groups were as follows: (1) control, basal diet; (2) treatment 1, treatment 2, and treatment 3, which were basal diet with 80, 160, and 240 mg/kg PCP, respectively. The results showed that compared with the control group, the feeding of anthocyanins significantly (p < 0.05) increased the average daily feed intake and average daily gain in chickens. Moreover, chickens receiving 80 mg/kg PCP significantly increased (p < 0.05) plasma total antioxidant capacity, superoxide dismutase, glutathione peroxidase, catalase, high-density lipoprotein cholesterol, and albumin concentrations relative to the control group. For meat quality, dietary supplementation with PCP significantly (p < 0.05) reduced the drip loss and water loss rate in breast muscle. Additionally, chickens receiving PCP tended to increase (p < 0.05) the levels of most individual amino acids, essential amino acids, and umami amino acids in the muscle. Specifically, the addition of 80 mg/kg PCP significantly improved (p < 0.05) total polyunsaturated fatty acids in chicken muscle. Accordingly, the consumption of anthocyanin-rich PCP by the growing chickens had the potential to increase the growth performance, enhance antioxidant and immune capacities, increase meat quality, and improve essential and umami amino acids as well as unsaturated fatty acids in the muscle.

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“…Similarly, in a study with cherry by-products, it was demonstrated that the aqueous infusion of the stems was the most active against this enzyme (IC50 = 3.18 ± 0.23 μg/mL) [ 159 ]. In the in vitro study conducted by Ji et al [ 156 ], it was observed that the anthocyanin-rich bilberry extract (BE) was able to inhibit the activity of α-glucosidase and α-amylase enzymes (IC 50 = 0.31 ± 0.02 e 4.06 ± 0.12 mg/mL, respectively). Moreover, in the same study the hypoglycemic effect of BE extract on postprandial glucose was evaluated.…”

Section: Functional Properties Of Cherries and Blueberries—focus On A...mentioning

confidence: 99%

“…Moreover, in the same study the hypoglycemic effect of BE extract on postprandial glucose was evaluated. The obtained results demonstrated that this extract was able to decrease hyperglycemia in the treated group with BE when compared to the control group [ 156 ]. In another work, fifteen different cultivars of Vaccinium corymbosum exhibited greater inhibitory activity against α-glucosidase with values ranging between 103.22 ± 0.15% and 184.70 ± 0.01% inhibition, and α-amylase ranging from 91.79 ± 0.82% and 103.32 ± 0.34% inhibition [ 157 ].…”

Section: Functional Properties Of Cherries and Blueberries—focus On A...mentioning

confidence: 99%

Cherries and Blueberries-Based Beverages: Functional Foods with Antidiabetic and Immune Booster Properties

et al. 2022

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Nowadays, it is largely accepted that the daily intake of fruits, vegetables, herbal products and derivatives is an added value in promoting human health, given their capacity to counteract oxidative stress markers and suppress uncontrolled pro-inflammatory responses. Given that, natural-based products seem to be a promising strategy to attenuate, or even mitigate, the development of chronic diseases, such as diabetes, and to boost the immune system. Among fruits, cherries and blueberries are nutrient-dense fruits that have been a target of many studies and interest given their richness in phenolic compounds and notable biological potential. In fact, research has already demonstrated that these fruits can be considered functional foods, and hence, their use in functional beverages, whose popularity is increasing worldwide, is not surprising and seem to be a promising and useful strategy. Therefore, the present review reinforces the idea that cherries and blueberries can be incorporated into new pharmaceutical products, smart foods, functional beverages, and nutraceuticals and be effective in preventing and/or treating diseases mediated by inflammatory mediators, reactive species, and free radicals.

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In vitro and in vivo inhibitory effect of anthocyanin-rich bilberry extract on α-glucosidase and α-amylase

Cited by 54 publications

References 45 publications

Bioreactor-grown exo- and endo-β-glucan from Malaysian Ganoderma lucidum: An in vitro and in vivo study for potential antidiabetic treatment

Bioreactor-grown exo- and endo-β-glucan from Malaysian Ganoderma lucidum: An in vitro and in vivo study for potential antidiabetic treatment

The Effects of Purple Corn Pigment on Growth Performance, Blood Biochemical Indices, Meat Quality, Muscle Amino Acids, and Fatty Acids of Growing Chickens

Cherries and Blueberries-Based Beverages: Functional Foods with Antidiabetic and Immune Booster Properties

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