In Vitro and In Vivo Evaluation of Lactobacillus delbrueckii subsp. bulgaricus KLDS1.0207 for the Alleviative Effect on Lead Toxicity

Abstract

Lead (Pb) is a toxic contaminating heavy metal that can cause a variety of hazardous effects to both humans and animals. In the present study, Lactobacillus delbrueckii subsp. bulgaricus KLDS1.0207 (L. bulgaricus KLDS1.0207), which has a remarkable Pb binding capacity and Pb tolerance, was selected for further study. It was observed that the thermodynamic and kinetic model of L. bulgaricus KLDS1.0207 Pb binding respectively fit with the Langmuir–Freundlich model and the pseudo second-order kinetic model. Scanning electron microscopy and energy dispersive spectroscopy analysis disclosed that the cell surfaces were covered with Pb and that carbon and oxygen elements were chiefly involved in Pb binding. Combined with Fourier transform infrared spectroscopy analysis, it was revealed that the carboxyl, phosphoryl, hydroxyl, amino and amide groups were the main functional groups involved in the Pb adsorption. The protective effects of L. bulgaricus KLDS1.0207 against acute Pb toxicity in mice was evaluated by prevention and therapy groups, the results in vivo showed that L. bulgaricus KLDS1.0207 treatment could reduce mortality rates, effectively increase Pb levels in the feces, alleviate tissue Pb enrichment, improve the antioxidant index in the liver and kidney, and relieve renal pathological damage. Our findings show that L. bulgaricus KLDS1.0207 can be used as a potential probiotic against acute Pb toxicity.

Bioremediation of lead and cadmium and the strive role of Pediococcus pentosaceus probiotic

“…Similarly, Bhakta et al (36) proved ability of six isolated from Pediococcus dextrinicus and Pediococcus acidilactici to tolerance, high concentration of Pb <1000, for all isolated, where's the MIC value for a Cd range from 50 to 250 for the six isolates. Li et al (37) reported that the MIC value for Pb by L. bulgaricus KLDS10207 was >1000. Since the bacteria under study showed high tolerance for both Pb and Cd, so they were used in the study of removing of these two metals using different concentration 25 and 50 ppm, and during 24 hours.…”

Comparative Assessment of the Bioremedial Potentials of Potato Resistant Starch-Based Microencapsulated and Non-encapsulated Lactobacillus plantarum to Alleviate the Effects of Chronic Lead Toxicity

“…The ability of seven KLDS strains to bind the Pb was investigated as formerly described by Li et al (2017) , with little amendments. Incubation of all strains was carried out for 16 h, and the centrifugation of the cultivated biomass was done at 10,000 g for 10 min at 4°C.…”

“…The kidney and liver tissues were split into 5 μm thickness with rotary microtome and fixed in paraffin. These sliced tissues were stained with hematoxylin-eosin and then observed by light microscopy ( Li et al, 2017 ).…”

“… Bhakta et al (2012) , Tian et al (2012) , and Zhai et al (2013 , 2015 ), conducted studies on the healing effects of oral administration of the LABs’ and their shielding effect against Pb noxiousness by reducing Pb levels in the tissues and blood and, impeding Pb-induced oxidative strain. The study conducted by Li et al (2017) has also provided the information that some Lactobacillus strains possess a positive impact to alleviate the acute Pb toxicity. In our previously published work ( Muhammad et al, 2017 ), we successfully incorporated PRS for the microencapsulation of Lactobacillus Plantarum KLDS 1.0344.…”

Phenotypic, fermentation characterization, and resistance mechanism analysis of bacteriophage-resistant mutants of Lactobacillus delbrueckii ssp. bulgaricus isolated from traditional Chinese dairy products

“…In previous studies, we identified a series of wild L. bulgaricus strains preserved by the Dairy Industrial Culture Collection, Key Lab of Dairy Science, Ministry of Education, China. These strains were obtained from Chinese traditional fermented dairy products, and they exhibited excellent flavor, viscosity, postacidification production characteristics, and key enzyme expression features (Liu et al, 2013;Hou et al, 2015;Li et al, 2017). However, their bacteriophage-resistance capacities have not been studied before.…”