“…FTIR spectra are widely sensitive to the secondary structure of silk fibroins, providing molecular validation of the change in silk structure. , The signature absorption peaks for WFS are found around 1620 cm –1 (Amide I), 1515 cm –1 (Amide II), and 1260 cm –1 (Amide III). These peaks are conformation peaks of the crystalline β sheet structure, and the silk shows similar peaks in all degumming conditions until the core structure starts to break . The Amide I band of the silk fibroin (between 1600 and 1700 cm –1 ) is mainly associated with C=O stretching vibration (70 to 85%) and is directly related to the backbone conformation.…”
Silkworm silk proteins
are of great importance in several fields
of science owing to their outstanding properties. India generates
waste silk fibers, also known as waste filature silk, in abundance.
Utilizing waste filature silk as reinforcement in biopolymers enhances
its physiochemical properties. However, the hydrophilic sericin layer
on the surface of the fibers makes it very difficult to have proper
fiber–matrix adhesion. Thus, degumming the fiber surface allows
better control of the fiber properties. The present study uses filature
silk (Bombyx mori) as fiber reinforcement
to prepare wheat gluten-based natural composites for low-strength
green applications. The fibers were degummed in sodium hydroxide (NaOH)
solution from a 0 to 12 h duration, and composites were prepared from
them. The analysis exhibited optimized fiber treatment duration and
its effect on the composite properties. The traces of the sericin
layer were found before 6 h of fiber treatment, which interrupted
homogeneous fiber–matrix adhesion in the composite. The X-ray
diffraction study showed enhanced crystallinity of the degummed fibers.
The FTIR study of the prepared composites with degummed fibers showed
that shifted peaks toward lower wavenumbers supported better bonding
among the constituents. Similarly, the tensile and impact strength
of the composite made of 6 h of degummed fibers showed better mechanical
properties than others. The same can be validated with the SEM analysis
and TGA as well. This study also showed that prolonged exposure to
alkali solution reduces the fiber properties, thus reducing composite
properties too. As a green alternative, the prepared composite sheets
can potentially be applied in manufacturing seedling trays and one-time
nursery pots.
“…FTIR spectra are widely sensitive to the secondary structure of silk fibroins, providing molecular validation of the change in silk structure. , The signature absorption peaks for WFS are found around 1620 cm –1 (Amide I), 1515 cm –1 (Amide II), and 1260 cm –1 (Amide III). These peaks are conformation peaks of the crystalline β sheet structure, and the silk shows similar peaks in all degumming conditions until the core structure starts to break . The Amide I band of the silk fibroin (between 1600 and 1700 cm –1 ) is mainly associated with C=O stretching vibration (70 to 85%) and is directly related to the backbone conformation.…”
Silkworm silk proteins
are of great importance in several fields
of science owing to their outstanding properties. India generates
waste silk fibers, also known as waste filature silk, in abundance.
Utilizing waste filature silk as reinforcement in biopolymers enhances
its physiochemical properties. However, the hydrophilic sericin layer
on the surface of the fibers makes it very difficult to have proper
fiber–matrix adhesion. Thus, degumming the fiber surface allows
better control of the fiber properties. The present study uses filature
silk (Bombyx mori) as fiber reinforcement
to prepare wheat gluten-based natural composites for low-strength
green applications. The fibers were degummed in sodium hydroxide (NaOH)
solution from a 0 to 12 h duration, and composites were prepared from
them. The analysis exhibited optimized fiber treatment duration and
its effect on the composite properties. The traces of the sericin
layer were found before 6 h of fiber treatment, which interrupted
homogeneous fiber–matrix adhesion in the composite. The X-ray
diffraction study showed enhanced crystallinity of the degummed fibers.
The FTIR study of the prepared composites with degummed fibers showed
that shifted peaks toward lower wavenumbers supported better bonding
among the constituents. Similarly, the tensile and impact strength
of the composite made of 6 h of degummed fibers showed better mechanical
properties than others. The same can be validated with the SEM analysis
and TGA as well. This study also showed that prolonged exposure to
alkali solution reduces the fiber properties, thus reducing composite
properties too. As a green alternative, the prepared composite sheets
can potentially be applied in manufacturing seedling trays and one-time
nursery pots.
“…If the vascular graft degrades too fast, rupture or aneurysm formation may occur [ 60 ]. Tissue regeneration may be inhibited by slow degradation and slow degradation may result in long-term inflammatory [ 61 ]. Therefore, the degradation rate of silk grafts should be adjusted to match the new tissue regeneration [ 62 , 63 ].…”
Section: Discussionmentioning
confidence: 99%
“…In our study, we found that the braided silk fibers remained visible even after 2 years; these fibers may play a role in maintaining long-term compliance. Studies have shown that the degradability of silk fibers could be altered by degumming conditions and could be controlled to match the diverse needs of specific tissue regeneration requirements [ 61 , 69 ]. Future studies may focus on adjusting the degradation rate of the braided silk fibers to match the new vascular regeneration.…”
The development of small-diameter vascular grafts that can meet the long-term patency required for implementation in clinical practice presents a key challenge to the research field. Although techniques such as the braiding of scaffolds can offer a tunable platform for fabricating vascular grafts, the effects of braided silk fiber skeletons on the porosity, remodeling, and patency in vivo have not been thoroughly investigated. Here, we used finite element analysis of simulated deformation and compliance to design vascular grafts comprised of braided silk fiber skeletons with three different degrees of porosity. Following the synthesis of low-, medium-, and high-porosity silk fiber skeletons, we coated them with hemocompatible sulfated silk fibroin sponges and then evaluated the mechanical and biological functions of the resultant silk tubes with different porosities. Our data showed that high-porosity grafts exhibited higher elastic moduli and compliance but lower suture retention strength, which contrasted with low-porosity grafts. Medium-porosity grafts offered a favorable balance of mechanical properties. Short-term in vivo implantation in rats indicated that porosity served as an effective means to regulate blood leakage, cell infiltration, and neointima formation. High-porosity grafts were susceptible to blood leakage, while low-porosity grafts hindered graft cellularization and tended to induce intimal hyperplasia. Medium-porosity grafts closely mimicked the biomechanical behaviors of native blood vessels and facilitated vascular smooth muscle layer regeneration and polarization of infiltrated macrophages to the M2 phenotype. Due to their superior performance and lack of occlusion, the medium-porosity vascular grafts were evaluated in long-term (24-months) in vivo implantation. The medium-porosity grafts regenerated the vascular smooth muscle cell layers and collagen extracellular matrix, which were circumferentially aligned and resembled the native artery. Furthermore, the formed neoarteries pulsed synchronously with the adjacent native artery and demonstrated contractile function. Overall, our study underscores the importance of braided silk fiber skeleton porosity on long-term vascular graft performance and will help to guide the design of next-generation vascular grafts.
“…Significantly, the degradation rate of silk fibroin hydrogels is highly dependent on the presence of ß-sheet structure. 45 It is noted that the native silk fibers show a lower degradation rate than regenerated silk fibroin fibers, which is ascribed to the higher content of ß-sheet secondary structure of natural silk fibers than that of RSF structure. Lu et al proposed that the hydrophilic bulk of silk fibroin was first degraded during the degradation process, and then the hydrophobic crystallites got rid of the surrounding and binding of the hydrophilic bulk to become free particles, followed by the movement toward the protease solution.…”
Section: Biodegradabilitymentioning
confidence: 99%
“…Significantly, the degradation rate of silk fibroin hydrogels is highly dependent on the presence of ß ‐sheet structure 45 . It is noted that the native silk fibers show a lower degradation rate than regenerated silk fibroin fibers, which is ascribed to the higher content of ß ‐sheet secondary structure of natural silk fibers than that of RSF structure.…”
Section: Structures and Properties Of Silk Fibroinmentioning
Silk fibroin hydrogels occupy an essential position in the biomedical field due to their remarkable biological properties, excellent mechanical properties, flexible processing properties, as well as abundant sources and low cost. Herein, we introduce the unique structures and physicochemical characteristics of silk fibroin, including mechanical properties, biocompatibility, and biodegradability. Then, various preparation strategies of silk fibroin hydrogels are summarized, which can be divided into physical cross‐linking and chemical cross‐linking. Emphatically, the applications of silk fibroin hydrogel biomaterials in various biomedical fields, including tissue engineering, drug delivery, and wearable sensors, are systematically summarized. At last, the challenges and future prospects of silk fibroin hydrogels in biomedical applications are discussed.
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