In vitro and in vivo leishmanicidal activity of Dysoxylum binectariferum and its fractions against Leishmania donovani

Lakshmi, Vijai; Pandey, Kartikey; Kapil, A.; Singh, Neeloo; Samant, Mukesh; Dube, Anuradha

doi:10.1016/j.phymed.2005.10.002

Cited by 66 publications

(31 citation statements)

References 15 publications

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“…The sensitivity of strain Dd8 and clinical isolates to Sb V (SAG; Albert David) was investigated against intramacrophage amastigotes as described earlier (9). Briefly, the mouse macrophage adherent cell line J774A.1 (1 ϫ 10 5 cells/ well) (Nunc) was cultured in 8-well chamber slides and infected with stationary-stage promastigotes at a ratio of 10:1 (L. donovani/macrophage) and was incubated in 5% CO 2 for 12 h at 37°C.…”

Section: Animals Laboratory-bred Male Golden Hamsters (Mesocricetus mentioning

confidence: 99%

Characterization of the Proliferating Cell Nuclear Antigen of Leishmania donovani Clinical Isolates and Its Association with Antimony Resistance

Tandon

Chandra

Baharia

et al. 2014

Antimicrob Agents Chemother

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Previously, through a proteomic analysis, proliferating cell nuclear antigen (PCNA) was found to be overexpressed in the sodium antimony gluconate (SAG)-resistant clinical isolate compared to that in the SAG-sensitive clinical isolate of Leishmania donovani. The present study was designed to explore the potential role of the PCNA protein in SAG resistance in L. donovani. For this purpose, the protein was cloned, overexpressed, purified, and modeled. Western blot (WB) and real-time PCR (RT-PCR) analyses confirmed that PCNA was overexpressed by >3-fold in the log phase, stationary phase, and peanut agglutinin isolated procyclic and metacyclic stages of the promastigote form and by ϳ5-fold in the amastigote form of the SAG-resistant isolate compared to that in the SAG-sensitive isolate. L. donovani PCNA (LdPCNA) was overexpressed as a green fluorescent protein (GFP) fusion protein in a SAG-sensitive clinical isolate of L. donovani, and modulation of the sensitivities of the transfectants to pentavalent antimonial (Sb V ) and trivalent antimonial (Sb III ) drugs was assessed in vitro against promastigotes and intracellular (J774A.1 cell line) amastigotes, respectively. Overexpression of LdPCNA in the SAG-sensitive isolate resulted in an increase in the 50% inhibitory concentrations (IC 50 ) of Sb V (from 41.2 ؎ 0.6 g/ml to 66.5 ؎ 3.9 g/ml) and Sb III (from 24.0 ؎ 0.3 g/ml to 43.4 ؎ 1.8 g/ml). Moreover, PCNA-overexpressing promastigote transfectants exhibited less DNA fragmentation compared to that of wild-type SAG-sensitive parasites upon Sb III treatment. In addition, SAG-induced nitric oxide (NO) production was found to be significantly inhibited in the macrophages infected with the transfectants compared with that in wild-type SAG-sensitive parasites. Consequently, we infer that LdPCNA has a significant role in SAG resistance in L. donovani clinical isolates, which warrants detailed investigations regarding its mechanism.

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Section: Animals Laboratory-bred Male Golden Hamsters (Mesocricetus mentioning

confidence: 99%

Characterization of the Proliferating Cell Nuclear Antigen of Leishmania donovani Clinical Isolates and Its Association with Antimony Resistance

Tandon

Chandra

Baharia

et al. 2014

Antimicrob Agents Chemother

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show abstract

“…For in vivo testing, required quantity of the extract/fractions were weighed, and a suspension was made in Tween 20 or 10% ethanol or saline water by crushing them in a pestle mortar, and final concentrations were made with sterile water. Test materials were administered as per standard procedures reported earlier [19].…”

Section: Preparation Of Stock Solutions and Administration Of Test Samentioning

confidence: 99%

“…donovani-infected hamsters were administered with 500 mg/kg, 250mg/kg and 100mg/kg of test samples by oral route. Miltefosine, the reference drug, was administered orally at a dose of 40 mg/ kg for 5 d. Splenic parasite loads in treated and untreated animals were determined on Day 7 post-treatment by performing splenic biopsies [19]. Impression smears were made on glass slides, air dried, fixed in absolute methanol, and stained with 10% Giemsa for 30 min.…”

Section: Leishmanicidal Efficacy Against Experimental Vl In Golden Hamentioning

confidence: 99%

Antileishmanial potential of a marine sponge Haliclona oculata against experimental visceral leishmaniasis

2015

JCLM

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“…f. (Maliaceae) and Amoora rohituka . It had earlier been reported for various biological activities, namely anti-infl ammatory, anticancer, immunomodulatory, and antiulcer properties (15)(16)(17)(18)(19)(20). Two of its analogs, Flavopiridol and P-276-00, are currently being evaluated in advanced phase 2 clinical trials for potential anticancer therapy ( 15 ).…”

Section: Differentiation Of 3t3-l1 and C3h10t1/2 Adipocytesmentioning

confidence: 99%

Rohitukine inhibits in vitro adipogenesis arresting mitotic clonal expansion and improves dyslipidemia in vivo

Varshney

Shankar

Beg

et al. 2014

Journal of Lipid Research

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In vitro and in vivo leishmanicidal activity of Dysoxylum binectariferum and its fractions against Leishmania donovani

Cited by 66 publications

References 15 publications

Characterization of the Proliferating Cell Nuclear Antigen of Leishmania donovani Clinical Isolates and Its Association with Antimony Resistance

Characterization of the Proliferating Cell Nuclear Antigen of Leishmania donovani Clinical Isolates and Its Association with Antimony Resistance

Antileishmanial potential of a marine sponge Haliclona oculata against experimental visceral leishmaniasis

Rohitukine inhibits in vitro adipogenesis arresting mitotic clonal expansion and improves dyslipidemia in vivo

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