In vitro and In vivo characterization of quercetin loaded multiphase hydrogel for wound healing application

Jangde, Rajendra; Srivastava, Shikha; Singh, Manju Rawat; Singh, Deependra

doi:10.1016/j.ijbiomac.2018.05.010

Cited by 72 publications

(42 citation statements)

References 27 publications

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“…Quercetin is another well-known natural compound due to its strong antioxidant properties and belongs to the class of flavonoids. A recent study developed from a multiphase system containing quercetin-loaded liposomes showed a significant reduction of lesion area in rats and confirmed its potential in the skin wound treatment [ 8 ].…”

Section: Introductionmentioning

confidence: 94%

Lupeol, a Pentacyclic Triterpene, Promotes Migration, Wound Closure, and Contractile Effect In Vitro: Possible Involvement of PI3K/Akt and p38/ERK/MAPK Pathways

et al. 2018

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Skin wound healing is a dynamic and complex process involving several mediators at the cellular and molecular levels. Lupeol, a phytoconstituent belonging to the triterpenes class, is found in several fruit plants and medicinal plants that have been the object of study in the treatment of various diseases, including skin wounds. Various medicinal properties of lupeol have been reported in the literature, including anti-inflammatory, antioxidant, anti-diabetic, and anti-mutagenic effects. We investigated the effects of lupeol (0.1, 1, 10, and 20 μg/mL) on in vitro wound healing assays and signaling mechanisms in human neonatal foreskin keratinocytes and fibroblasts. Results showed that, at high concentrations, Lupeol reduced cell proliferation of both keratinocytes and fibroblasts, but increased in vitro wound healing in keratinocytes and promoted the contraction of dermal fibroblasts in the collagen gel matrix. This triterpene positively regulated matrix metalloproteinase (MMP)-2 and inhibited the NF-κB expression in keratinocytes, suggesting an anti-inflammatory effect. Lupeol also modulated the expression of keratin 16 according to the concentration tested. Additionally, in keratinocytes, lupeol treatment resulted in the activation of Akt, p38, and Tie-2, which are signaling proteins involved in cell proliferation and migration, angiogenesis, and tissue repair. These findings suggest that lupeol has therapeutic potential for accelerating wound healing.

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Section: Introductionmentioning

confidence: 94%

Lupeol, a Pentacyclic Triterpene, Promotes Migration, Wound Closure, and Contractile Effect In Vitro: Possible Involvement of PI3K/Akt and p38/ERK/MAPK Pathways

et al. 2018

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“…e chemical characterization of plant showed that the extract of J. decurrens Cham. presents compounds of the class of flavonoids, tannins, and triterpenes, which have been reported with anti-inflammatory, antioxidant, and healing activities [32][33][34][35][36]. e inflammation modulation is important to reduce the damage resulting from proteases release by neutrophils, such as excessive degradation of extracellular matrix and proteins important for tissue repair, besides the free radicals production [37].…”

Section: μMmentioning

confidence: 99%

Chemical Characterization and Wound Healing Property of Jacaranda decurrens Cham. (Bignoniaceae): An Experimental Study Based on Molecular Mechanisms

Serra

Barroso

Rocha

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. Jacaranda decurrens Cham., known as carobinha, is prevalent in the Cerrado biome and presents popular use in treatment of dermatological diseases. The present study aimed to investigate the healing action of topical formulation of Jacaranda decurrens Cham. (FtEHJ) in mice cutaneous lesions. Methods. Phytochemical analysis of J. decurrens hydroalcoholic extract was carried out by using HPLC-PDA-ESI-MS and FIA-ESI-IT-MSn. Swiss mice were treated topically with formulation base (FtB) or Fibrinase® or ointment FtEHJ (15 mg/g; 50 mg/Kg). At the end of treatment periods, the inflammatory cytokines (TNF-α, IL-1β, and IL-6) in the lesions were measured by using ELISA and gene expression of TGF-β, Collagen I, and Collagen III was demonstrated by RTqPCR method and histological evaluation. Results. Ten compounds were identified in the extract, distributed among the classes of flavonoids and triterpenes. Treatment with FtEHJ increased the wound contraction in 24 hours, such as reduction of TNF-α, IL-1β, and IL-6 (pg/mL) cytokines in the lesion. The TGF-β and collagen gene expression was increased and the wound closure accelerated to nine days, with discrete inflammation, collagenization, and accented reepithelialization. Conclusions. The results obtained suggest chemical compounds present in the FtEHJ accelerates wound healing by being a gene expression modulator, and protein content of different molecules are involved in tissue repair.

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“…Multiphase hydrogel system incorporated with quercetin loaded liposomes for wound healing applications has been fabricated as well. The prepared hydrogel combines the effect to control the release of quercetin with haemocompatibility and good mechanical properties as well as accelerates wound healing [15].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Antitumor Activities of Novel Quercetin-Loaded Electrospun Cellulose Acetate/Polyethylene Glycol Fibrous Materials

et al. 2020

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The aim of present study was to obtain novel fibrous materials based on cellulose derivative and polyethylene glycol loaded with natural biologically active compound quercetin by electrospinning. Several methods including scanning electron microscopy (SEM), IR spectroscopy, X-ray diffraction analysis (XRD), water contact angle measurements, differential scanning calorimetry (DSC), and UV-VIS spectroscopy were utilized to characterize the obtained materials. The incorporation of polyethylene glycol in the fibrous material resulted in increased hydrophilicity and burst release of quercetin from the fibers. Quercetin-containing fibrous mats exhibited high antioxidant activity as estimated by DPPH free radical scavenging method. In vitro tests with HeLa tumor cells and SH-4 melanoma skin cells were performed in order to determine the cytotoxicity of the novel materials. It was found that the fibrous CA/PEG/QUE materials exhibited high cytotoxic effect against both cell lines. Therefore, the novel polymeric materials containing quercetin are promising candidates for biomedical and pharmaceutical applications.

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In vitro and In vivo characterization of quercetin loaded multiphase hydrogel for wound healing application

Cited by 72 publications

References 27 publications

Lupeol, a Pentacyclic Triterpene, Promotes Migration, Wound Closure, and Contractile Effect In Vitro: Possible Involvement of PI3K/Akt and p38/ERK/MAPK Pathways

Lupeol, a Pentacyclic Triterpene, Promotes Migration, Wound Closure, and Contractile Effect In Vitro: Possible Involvement of PI3K/Akt and p38/ERK/MAPK Pathways

Chemical Characterization and Wound Healing Property of Jacaranda decurrens Cham. (Bignoniaceae): An Experimental Study Based on Molecular Mechanisms

Antioxidant and Antitumor Activities of Novel Quercetin-Loaded Electrospun Cellulose Acetate/Polyethylene Glycol Fibrous Materials

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