In Vitro and In Vivo Inhibitory Effect of Gujin Xiaoliu Tang in Non‐Small Cell Lung Cancer

Abstract

Non-small cell lung cancer (NSCLC) is a serious threat to people's health. This study aims to determine the possible effect of Gujin Xiaoliu Tang (GJXLT) on NSCLC, which is an empirical formula from Professor Dai-Han Zhou. In this study, chromatographic fingerprinting of GJXLT and A549 cell model in vitro and in vivo was established. We cultured A549 cells in vitro and found that GJXLT inhibited A549 cell growth and induced apoptosis. Compared with the control group, the expression of p-STAT3 and VEGF proteins in the GJXLT groups was decreased. Similar findings were also observed in vivo. First, GJXLT inhibited the growth of transplanted tumor and did not reduce the weight of the tumor-bearing mice in comparison with that of the control group. Then, the Ki-67 expression of transplanted tumor in the GJXLT groups was decreased. In addition, the apoptosis rate of transplanted tumor in the GJXLT groups was increased. Overall, our data showed that GJXLT inhibited A549 cell proliferation and induced apoptosis in vivo and in vitro. Furthermore, GJXLT inhibited the growth of lung cancer xenograft in nude mice model with no obvious side effects. The anti-tumor effect of GJXLT might also be related to the inhibition of p-STATS and VEGF expression in the JAK2/STAT3 pathway. Our results demonstrated the potential of GJXLT as a novel treatment for NSCLC.

Huang Lian Jie Du Decoction enhances the anti-tumor efficacy of immune checkpoint inhibitors by activating TLR7/8 signalling in melanoma

“…Traditional Chinese Medicine (TCM) has been shown to have efficacy in improving life quality and prolonging the survival time in cancer patients [ 14 , 15 ], and enhancing the response of radiotherapy, chemotherapy, targeted therapy and photodynamic therapy to cancer [ 16 – 20 ]. Huang Lian Jie Du Decoction (HLJD, Oren-gedoku-to in Japanese, Hwangryunhaedok-tang in Korean), a classical TCM formula with anti-inflammatory effects, has been widely used as combination therapy for cancer [ 21 , 22 ], arthritis [ 23 ], cerebral ischemia/reperfusion [ 24 , 25 ], type II diabetes [ 26 , 27 ], and atopic dermatitis [ 28 ] in China [ 29 ].…”

Exploring the Mechanism of Inula japonica Thunb. against Non-small Cell Lung Cancer using a computer-aided drug design approach

“…Traditional Chinese medicine (TCM) in combination with chemotherapy is routinely recommended for patients with NSCLC which acts as therapeutic effect through regulation of multiple compounds, targets, and pathways [11,12]. In addition, TCM can not only relieve chemotherapy-related side effect but also prolong the survival time of patients [13].…”

Network pharmacological analysis of active components of Xiaoliu decoction in the treatment of glioblastoma multiforme

“…A large number of clinical studies have demonstrated that TCM can be combined with surgery, radiotherapy and chemotherapy to achieve better results, prevent tumor progression, alleviate clinical symptoms and reduce adverse reactions of radiotherapy and chemotherapy, thereby improving the quality of life and prognosis of patients ( The Lancet Oncology, 2015 ). For example, Xiaoliu decoction (XLD) has been widely used in China and achieved definite curative effects in colorectal cancer, liver cancer, pancreatic cancer, non-small cell lung cancer and glioma ( Fu and Xia, 2004 ; Wang et al, 2004 ; Hou et al, 2018 ). The recommended formula mainly includes Baihuasheshecao (15 g), baizhi (10 g), Baizhu (15 g), banxia (10 g), Banzhilian (15 g), chenpi (10 g), Chuanxiong (10 g), Danshen (15 g), Fuling (15 g), Gancao (6 g), Gouteng (10 g) and Yanhusuo (10 g).…”