In vitro and in vivo biocompatibility of Ti-6Al-4V titanium alloy and UHMWPE polymer for total hip replacement

Vu, Ngoc Bich; Truong, Nhung Hai; Dang, Long Thanh; Phi, Lan Thi; Ho, Nga Thi-Thu; Pham, Tuan; Phan, Trinh Phuong; Pham, Phuc Van

doi:10.7603/s40730-016-0014-8

Cited by 19 publications

(13 citation statements)

References 22 publications

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“…Ti6Al4V-ELI (per ASTM F136) material, which has been widely used and already verified [11,12] as a medical device material for orthopedics and neurosurgery to improve bone union rate, was selected, and the porous structure and size confirmed through animal experiments were applied. The porous structure of the spinal fusion cage is advantageous for increasing the bone union rate, but due to the unique rough surface, there is a risk of damage to the nerves and blood vessels during the insertion of the cage in surgery.…”

Section: Design and Prototyping Of Porous Compound Cagementioning

confidence: 99%

Design and Biomechanical Verification of Additive Manufactured Composite Spinal Cage Composed of Porous Titanium Cover and PEEK Body

et al. 2019

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Incidents of lumbar degenerative diseases, such as spinal stenosis and degenerative spondylolisthesis, are increasing due to the aging population, and as a result, posterior lumbar interbody fusion (PLIF) is widely used. However, the interbody fusion cage used in the fusion surgery has been reported to cause subsidence in the fusion cage of the titanium material and bone nonunion in the case of the polyetheretherketone (PEEK) material cage. Therefore, we aim to reduce the possibility of subsidence of the spinal fusion cage through its elastic modulus difference with the cortical bone of the vertebral body. For the vertebral end plate, which is related to the fusion rate, we also aim to design a new composite vertebral cage, which integrates a cover of porous structure using the additive manufacturing method of titanium alloy to fabricate a prototype, and to biomechanically verify the prototype. The method was as follows. In order to find a similar pore size of human cancellous bone, the pore size was adjusted and the results were measured with SEM. The pore size of each surface was measured individually and the mean value was calculated. Next, an animal experiment was conducted to confirm the degree of fusion of each structural type, and prototypes of various structures were fabricated. The degree of fusion was confirmed by a push down test. A prototype of the fusion cage composed of titanium and PEEK material was fabricated, and the possibility of subsidence by existence of porous structure was confirmed by using the lumbar spine finite element model. Then, the prototype was compared with the composite fusion cage developed by ASTM F2077 and ASTM F2267 methods, and with the commercial PEEK and titanium cages. As a result, the correlation between bone fusion and the porous structure, as well as size of the spine fusion cage composing the composite for porous structure and elasticity, was confirmed. Type 3 structures showed the best performance in bone fusion and the pore size of 1.2 mm was most suitable. In addition, the likelihood of subsidence of a cage with a porous structure was considered to be lower than that of a cage with a solid structure. When the new composite cage combined with two composites was compared with commercial products to verify, the performance was better than that of the existing PEEK material. The subsidence result was superior to the titanium product and showed similar results to PEEK products. In conclusion, the performance value was superior to the existing PEEK material, and the subsidence result was superior to the titanium product and was similar to the PEEK product, and thus, performance-wise, it is concluded that the PEEK product can be completely replaced with the new product.

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Section: Design and Prototyping Of Porous Compound Cagementioning

confidence: 99%

Design and Biomechanical Verification of Additive Manufactured Composite Spinal Cage Composed of Porous Titanium Cover and PEEK Body

et al. 2019

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“…Fibroblasts are usually not in direct contact with the implant surface, but have been widely accepted as one of the most sensitive cell lines for inflammatory reactions. Thus, we considered fibroblasts as an appropriate in vitro model to evaluate biocompatibility of DED specimens [31,32]. L929 cells were seeded in 24-well culture plates at a concentration of 5 × 10 3 cells per well and were cultured on day 1, 3, 5, and 7.…”

Section: Viability Analysis On Culture Of Fibroblastsmentioning

confidence: 99%

Titanium Porous Coating Using 3D Direct Energy Deposition (DED) Printing for Cementless TKA Implants: Does It Induce Chronic Inflammation?

et al. 2020

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Because of the recent technological advances, the cementless total knee arthroplasty (TKA) implant showed satisfactory implant survival rate. Newly developed 3D printing direct energy deposition (DED) has superior resistance to abrasion as compared to traditional methods. However, there is still concern about the mechanical stability and the risk of osteolysis by the titanium (Ti) nanoparticles. Therefore, in this work, we investigated whether DED Ti-coated cobalt-chrome (CoCr) alloys induce chronic inflammation reactions through in vitro and in vivo models. We studied three types of implant surfaces (smooth, sand-blasted, and DED Ti-coated) to compare their inflammatory reaction. We conducted the in vitro effect of specimens using the cell counting kit-8 (CCK-8) assay and an inflammatory cytokine assay. Subsequently, in vivo analysis of the immune profiling, cytokine assay, and histomorphometric evaluation using C57BL/6 mice were performed. There were no significant differences in the CCK-8 assay, the cytokine assay, and the immune profiling assay. Moreover, there were no difference for semi-quantitative histomorphometry analysis at 4 and 8 weeks among the sham, smooth, and DED Ti-coated samples. These results suggest that DED Ti-coated printing technique do not induce chronic inflammation both in vitro and in vivo. It has biocompatibility for being used as a surface coating of TKA implant.

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“…The cell line will be incubated at 37 ± 2°C in humidified 5% CO2 and 95.0 % air atmosphere. The cell line will be incubated to achieve approximately 80% confluency at 37 ± 2°C in humidified 5% (volume fraction) CO 2 and 95.0% air atmosphere as appropriate for the buffer system chosen for the culture medium for two to three days before the treatment [22].…”

Section: Cell Culturementioning

confidence: 99%

Bio-Compatible Processing of LENSTM DepositedCo-Cr-W alloy for Medical Applications

Suresh¹,

Narayana²,

Mallik³

2018

IJET

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Developing a Medicinal implants or devices is a challenging task for the researchers, right from the selection of materials, design, bio-compatibility and implantation to the host tissue. At every stage it requires proper care in processing of medical implants. In recent years the demand for medical implants had grown rapidly due to the awareness in the society. Major share of implants is used by younger people as they are active in sports, motor vehicle accidents leads to facture. Even older people also preferring to implants for ease of living. The commonly used implants are, prosthetic joints, knee replacement, dental, maxillofacial reconstructions etc.There is huge demand for the medical implants in coming years, presently a few bio-materials available for implant devices such as Ti-alloys, Stainless steel and Co-Cr-Mo alloys. There a scope to the researchers to develop a new alloy that are bio-compatible in nature and bring down the cost of the implant procedure to the needed patients. In this context additive manufacturing (AM) is an advanced manufacturing technology emerging as prominent technique in medical fields. Laser Engineered Net ShapingTM (LENS) is one such metal additive technique which provides fabrication of parts with the help of laser power, melts the powder alloy completely and builds parts layer by layer directly from the CAD model.In the present study, samples are fabricated from LENS process and carried the In-Vitro and In -Vivo bio-compatible tests as cytotoxicity and sub chronic toxicity to verify the toxicants release and their sustainability as the medical implants by the LENS deposited Co-Cr-W alloy samples. From the studies it is observed that the alloy samples show acceptable result. MTT assay demonstrate that cell viability is better in Osteoblast cells compared to the Fibroblast cells. Osteoblast cells show slightly more viable to the cell treatment on the samples during the experimental period. Sub chronic toxicity conclude that LENS deposited Co-Cr-W alloy is not toxic in all the rats studied herein and did not produce any toxic signs or evident symptoms. LENS deposited Co-Cr-W alloy did not cause any lethality or produce any relative body organs weight and haematological studies didn’t show adverse effects.

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In vitro and in vivo biocompatibility of Ti-6Al-4V titanium alloy and UHMWPE polymer for total hip replacement

Cited by 19 publications

References 22 publications

Design and Biomechanical Verification of Additive Manufactured Composite Spinal Cage Composed of Porous Titanium Cover and PEEK Body

Design and Biomechanical Verification of Additive Manufactured Composite Spinal Cage Composed of Porous Titanium Cover and PEEK Body

Titanium Porous Coating Using 3D Direct Energy Deposition (DED) Printing for Cementless TKA Implants: Does It Induce Chronic Inflammation?

Bio-Compatible Processing of LENSTM DepositedCo-Cr-W alloy for Medical Applications

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