In vitro and in vivo effects of selected metabolic inhibitors and chemotherapeutic agents on adults and egg development of Schistosoma mansoni

Lee, Harry G.; Michaels, Rhoda M.

doi:10.1016/0014-4894(68)90101-x

Cited by 17 publications

(3 citation statements)

References 27 publications

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“…A recent study (Lee & Michaels, 1968) of the effects on S. mansoni of certain 'metabolic inhibitors', namely actinomycin D r puromycin, chloramphenicol and amethopterin, showed that the last three compounds had no effect in vitro on adult worm viability, egg-laying, or development. Actinomycin D, a DNA inhibitor, had a selective in vitro action on the rate of oviposition and maturation of eggs, although inactive in vivo.…”

Section: Discussionmentioning

confidence: 99%

Experimental studies on the chemosterilization of Schistosoma mansoni

Davies

Jackson

1970

Parasitology

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Treatment of mice infected with S. mansoni by various sterilants effective against male rodents produced phases of inhibition of schistosome oviposition assessed by miracidial counts. In the treated worms, besides apparent disappearance of the testes, marked involution of ovarian tissue occurred. The gonads of both male and female worms seem to be directly affected by the antifertility substances. It is suggested that gamete production in both sexes follows similar lines in contrast to the finite reservoir of meiotic oocytes in mammals.

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Section: Discussionmentioning

confidence: 99%

Experimental studies on the chemosterilization of Schistosoma mansoni

Davies

Jackson

1970

Parasitology

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“…The best quantitative in vitro egg production data for adult S. mansoni are those of Schiller, Bueding, Turner & Fisher (1975), who obtained a mean total of 680 eggs/worm pair during a 12 day maintenance period using a diphasic medium. With daily egg production by adult S. mansoni in vivo estimated to be between 200 and 300 eggs/pair (Weinmann & Hunter, 1961;Kloetzel, 1967;Lee & Michaels, 1968), it is clear that total egg production in vitro does not exceed the equivalent of 2-3 days production at the estimated in vivo rate, and the majority of published in vitro data falls well below this level.…”

Section: Discussionmentioning

confidence: 99%

Schistosoma mansoni: effect of maintenancein vitroon the physiology and biochemistry of adult worms

Mercer

Chappell

1985

Parasitology

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In Medium 199 supplemented with calf serum (+ 10%), adult Schistosoma mansoni produced a mean total of 400 eggs/worm pair during maintenance for 10 days in vitro. During the period of egg deposition in vitro, the dry weight of worm pairs decreased by 35%. Biomass decreases were proportionally greater in female worms than in males. Declines in the dry weight of female worms were mainly due to decreases in protein content, while biomass decreases in males resulted from losses of both protein and glycogen. Glucose was depleted from the maintenance medium at a rate of approximately 200 μg/worm pair/day during each of the first 3 days in vitro, while concentrations of glucose in the medium of above 1 g/litre did not affect the degree of glycogen depletion observed in adult S. mansoni during culture for 24 h. The contribution of the loss of schistosome gut contents in vitro to the observed changes is discussed. The work described represents part of an attempt at logical design of in vitro culture.

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“…A number of papers have appeared in which the activity of individual drugs against schistosomes maintained in vitro has been reported (e.g. Foster and Cheetham, 1973;Lee, 1972;Lee and Michaels, 1968;Magzoub, 1972;McLure and Ercoli, 1971;Pellegrino and Maria, 1966) but there has been little attempt to see whether the methods used are applicable to screening. Young worms have not been used, although the possibility of using schistosomula in chemotherapeutic studies has been raised (Tiba et al t 1974).…”

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confidence: 99%