In vitro and in vivo experimental trials to assess the modulatory influence of β-caryophyllene on NDV replication and immunopathogenesis

Hassanin, Ola; Abdallah, Fatma; Galal, Azza A.A.

doi:10.1016/j.cimid.2020.101547

Cited by 9 publications

(7 citation statements)

References 23 publications

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“…Consistent with the normal incubation period of the velogenic ND ranging mostly between 2 and 7 days ( Parede and Young, 1990 ), the disease’s symptoms began to appear 51-h post-infection, and then raised in a rapid progressive manner in untreated control group until all the chickens died on day 9 post-infection ( Figures 1 , 2 ), as expected ( Ferreira et al, 2019 ). Unlike other reported compounds with no or low therapeutic effects on virus infection in vivo ( Mtambo et al, 1999 ; Hassanin et al, 2020 ), n -docosanol was found to largely increase both curative and survival rates by around 50% in the treated chickens, especially in the medium and high dosages. Interestingly, no significant variation in the curative and survival rates was observed between the treatments with the medium and high dosages of n -docosanol.…”

Section: Discussioncontrasting

confidence: 78%

Therapeutic efficacy of n-Docosanol against velogenic Newcastle disease virus infection in domestic chickens

et al. 2022

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IntroductionThe control of Newcastle disease virus (NDV) infection depends solely on vaccination which in most cases is not sufficient to restrain the consequences of such a highly evolving viral disease. Finding out substances for preparing an efficient anti-ND drug would be of high value. n-Docosanol is a saturated fatty alcohol with an inhibitory effect against many enveloped viruses. In this study, we evaluated the therapeutic effect of n-docosanol on NDV infection and shedding in chickens.MethodsChickens infected with a highly virulent NDV were treated with low to high concentrations of n-docosanol (20, 40, and 60 mg/kg body weight) for 4-successive days, once they showed the disease symptoms. Survival and curative rates, virus load, histopathological scoring, and virus shedding were defined.ResultsSymptoms development was found to discontinue 24–72 hours post-treatment. Survival rate in the NDV-infected chickens raised 37.4–53.2% after the treatment. n-Docosanol treatment was also found to significantly reduce virus load in the digestive (26.2–33.9%), respiratory (38.3–63%), nervous (26.7–51.1%), and lymphatic (16.4–29.1%) tissues. Histopathological scoring of NDV lesions revealed prominent rescue effects on the histology of different tissues. Importantly, n-docosanol treatment significantly reduced virus shedding in oropharyngeal discharge and feces thereby allowing the restriction of NDV spread.ConclusionOur findings suggest n-docosanol as a promising remedy in the control strategy of Newcastle disease in the poultry industry.

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Section: Discussioncontrasting

confidence: 78%

Therapeutic efficacy of n-Docosanol against velogenic Newcastle disease virus infection in domestic chickens

et al. 2022

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“…Antiviral activity of β-caryophyllene has been identified against different enveloped viruses, such as the herpes simplex virus, Newcastle disease virus, and avian infectious bronchitis virus (a gammacoronavirus) [ 19 , 20 , 21 ]. In addition, a mixture of compounds that includes β-caryophyllene has shown antiviral activity against the alphacoronavirus human coronavirus E229 [ 22 ].…”

Section: Resultsmentioning

confidence: 99%

Screening of Natural Products Inhibitors of SARS-CoV-2 Entry

et al. 2022

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The COVID-19 pandemic has led to the search for new molecules with antiviral activity against SARS-CoV-2. The entry of the virus into the cell is one of the main targets for inhibiting SARS-CoV-2 infection. Natural products are an important source of new therapeutic alternatives against diseases. Pseudotyped viruses allow the study of SARS-CoV-2 viral entry inhibitors, and due to their simplicity, they allow the screening of a large number of antiviral candidates in Biosafety Level 2 facilities. We used pseudotyped HIV-1 with the D614G SARS-CoV-2 spike glycoprotein to test its ability to infect ACE2-expressing HEK 293T cells in the presence of diverse natural products, including 21 plant extracts, 7 essential oils, and 13 compounds from plants and fungi. The 50% cytotoxic concentration (CC50) was evaluated using the resazurin method. From these analyses, we determined the inhibitory activity of the extract of Stachytarpheta cayennensis, which had a half-maximal inhibitory concentration (IC50) of 91.65 µg/mL, a CC50 of 693.5 µg/mL, and a selectivity index (SI) of 7.57, indicating its potential use as an inhibitor of SARS-CoV-2 entry. Moreover, our work indicates the usefulness of the pseudotyped-virus system in the screening of SARS-CoV-2 entry inhibitors.

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“…Carvacrol attenuated the excessive immune response induced by the influenza A virus by suppressing viral replication and recognizing TLR/retinoic acid-induced gene I-like receptor (RLR) patterns [116] . β-Caryophyllene improved damage induced by toxic Newcastle disease virus in birds by suppressing viral replication and regulating immunity to increase the level of interferon α (a signaling cytokine), exerting optimal therapeutic effects before or during infection [117] . Certain sesquiterpenoids in Chrysanthemum VO exhibit selective anti-viral activities.…”

Section: Anti-viral Effectsmentioning

confidence: 99%

Pharmacological effects of volatile oil from chrysanthemum and its associated mechanisms: a review

Zhang,

Su,

Filipczak

et al. 2024

Acupuncture and Herbal Medicine

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Volatile oil (VO) is the main chemical component of common plants in Chrysanthemum genus, and it possesses several beneficial pharmacological properties, including bacteriostatic, antioxidant, anti-tumor, anti-inflammatory, antipyretic, analgesic, anti-osteoporotic, antihypertensive, sedative, and hypnotic effects. To date, research on the effective components of Chrysanthemum extract has mainly focused on flavonoids, whereas limited data are available on the chemical constituents and underlying mechanisms of action of the VO components. In this review, the pharmacological activities and mechanisms of VO are comprehensively reviewed with the aim of providing a foundation for further development for medicinal, aromatherapy, and diet therapy applications.

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In vitro and in vivo experimental trials to assess the modulatory influence of β-caryophyllene on NDV replication and immunopathogenesis

Cited by 9 publications

References 23 publications

Therapeutic efficacy of n-Docosanol against velogenic Newcastle disease virus infection in domestic chickens

Therapeutic efficacy of n-Docosanol against velogenic Newcastle disease virus infection in domestic chickens

Screening of Natural Products Inhibitors of SARS-CoV-2 Entry

Pharmacological effects of volatile oil from chrysanthemum and its associated mechanisms: a review

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