In vitro and in vivo antioxidant effects of the ethanolic extract of Swertia chirayita

Chen, Yue; Huang, Bo; He, Jingsheng; Han, Lei; Zhan, Yichao; Wang, Youwei

doi:10.1016/j.jep.2011.04.058

Cited by 110 publications

(70 citation statements)

References 43 publications

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“…The phenolic and flavonoid content of the methanolic extract was found to be 9.20 ± 0.9 and 27.80 ± 1.4 respectively. Our results were in confirmation with studies carried out previously on other species of Swertia (12). Highest phenolic and flavonoid content in the methanolic extracts of Swertia corymbosa were also recorded (13).…”

Section: Total Phenolics and Flavonoid Contentsupporting

confidence: 92%

Evaluation of antimicrobial and antioxidant activities of Swertia petiolata

Rather¹

2016

Adv Biomed Pharma

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Swertia petiolata is considered as an important medicinal herb and has been used to treat different ailments by various ethnic groups in Kashmir. The present study was designed to evaluate the antioxidant and antimicrobial activity of methanolic extract of S. petiolata. The antioxidant activity was evaluated by three different assays namely diphenylpicrylhydrazyl (DPPH), nitroblue tetrazolium (NBT) and ferric reducing power (FRAP). The IC 50 values of the methanolic extract were found to be 240 ± 4.14, 258 ± 5.71 and 100 ± 3.53 for DPPH, NBT and FRAP, respectively. The antimicrobial activities were determined by agar diffusion and microdilution methods against Gram-positive (Bacillus subtilis ATCC 6051, Sarcina lutea ATCC 10054, Staphylococcus aureus ATCC 25932), Gram-negative (Escherichia coli ATCC 25922, Enterobacter cloacae ATCC 13883, Pseudomonas aeruginosa ATCC 27853) and yeast (Candida albicans ATCC 10231). The methanolic extract was found to be more active against Gram-positive bacteria as compared to Gram-negative bacteria and the yeast. The Minimum inhibitory concentration (MIC) values ranged from 67.5-125, 125-250 and 350μg/ml for Gram-positive, Gram-negative and C. albicans respectively. Taken together, we propose that the plant is a rich source of antioxidants and antimicrobials.

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Section: Total Phenolics and Flavonoid Contentsupporting

confidence: 92%

Evaluation of antimicrobial and antioxidant activities of Swertia petiolata

Rather¹

2016

Adv Biomed Pharma

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“…Numerous studies have reported many chemical compounds in S. chirayita such as chiratinin, terpenoids, iridoids gentianine, amarogentin, amaroswerin, xanthones, secoiridoid, glycosides and urosilic acid (Bajpai et al, 1991;Joshi and Dhawan, 2005;Khanal et al, 2015;Kumar and Chandra, 2015;Kshirsagar et al, 2016Kshirsagar et al, , 2017. These compounds and their derivatives possess antihepatotoxic, antileishmanial, anticarcinogenic, antioxidant, anti-inflammatory, antidiabetic, antimalarial and antihelmithic properties (Bajpai et al, 1991;Ray et al, 1996;Saha et al, 2004;Iqbal et al, 2006;Balaraju et al, 2009;Chen et al, 2011;Nagalekshmi et al, 2011;Phoboo et al, 2013;Zhou et al, 2015;Lad and Bhatnagar, 2016). Recently, Tupe et al (2017) have reported that S. chirayita showed antiglycating activity which plays crucial role in antidiabetic effects.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity in Nepalese population of Swertia chirayita (Roxb. Ex Fleming) H. Karst based on inter-simple sequence repeats (ISSR) markers

Neupane¹,

Sijapati²,

Bhattarai³

et al. 2017

Afr. J. Biotechnol.

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Swertia chirayita is a highly valued but vulnerable medicinal plant species of Nepal. Its populations are declining in natural habitats due to over exploitation. Twenty-seven inter-simple sequence repeats (ISSR) primers were used to assess the genetic diversity and population genetic structure of 42 genotypes representing six natural populations of S. chirayita, from Nepal. Of the total 479 bands amplified by 27 ISSR primers, 473 (98.18%) were polymorphic, indicating very high level of genetic diversity at species level. Percentage polymorphism value for different primers ranged from 83.3 to 100% with an average of 98.18%. Polymorphism information content (PIC) value ranged from 0.88 to 0.93 with an average of 0.91. Cluster analysis performed with NTSYS pc statistical package using Jaccard's similarity coefficients generated from ISSR binary data matrix showed that, all 27 ISSR primers separated 42 individuals into two major clusters and six sub clusters at the similarity level of 0.24. The average value of Nei's genetic diversity (H) and Shannon's information index (I) equaled 0.276 and 0.423, respectively at species level. The coefficient of genetic differentiation (G ST ) amongst populations of S. chirayita was found to be high (0.548) with restricted gene flow (N m =0.4829). Analysis of molecular variance showed that genetic diversity within populations is slightly higher (50.9%) than among populations (47.6%). The present genetic diversity assessment of S. chirayita populations has been of immense importance to understand the cause of its vulnerability and has furnished valuable insights for its conservation and sustainable utilization.

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“…x A. squamosa L.). Chen et al (2011). A solução de ABTS + foi preparada pela reação de ABTS 7 mM (5 mL) e 2,45 mM (88 µL) de persulfato de potássio, após incubação à temperatura ambiente no escuro, durante 16 h. Foi então diluída com etanol a 80% para se obter uma absorbância de 0,700 ± 0,005 a 734 nm.…”

Section: Introductionunclassified

“…Amostras de Trolox com as mesmas concentrações foram usadas como padrões. No método da cooxidação do β-caroteno frente ao ácido linoleico foi utilizada a metodologia descrita por Chen et al (2011).…”

Section: Introductionunclassified

Atividade antioxidante e antimicrobiana de extratos de atemoia (Annona cherimola Mill. x A. squamosa L.)

et al. 2014

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RESUMO -O objetivo deste trabalho foi quantificar os teores de fenóis e flavonoides totais, bem como avaliar as atividades antioxidante e antimicrobiana de extratos obtidos dos talos e folhas de atemoia (A. cherimola Mill. x A. squamosa L.), que pertence à família Annonaceae. A atividade antioxidante foi avaliada pelos métodos de sequestro dos radicais 2,2-difenil-1-picrilhidrazil (DPPH) e 2,2´-azinobis-3-etilbenzotiazolina-6-ácido sulfônico (ABTS), bem como pelo método da cooxidação do β-caroteno/ácido linoleico. A avaliação da atividade antimicrobiana dos extratos foi analisada contra 10 cepas de bactérias. Os resultados da atividade antioxidante dos extratos mostraram que o extrato etanólico dos talos (EEt) foi o antioxidante mais efetivo (IC 50 = 10,44 ± 1,25 µg/mL) no método do sequestro do DPPH, bem como no sequestro do radical ABTS (24,81 ± 0,49%). O extrato hexânico das folhas apresentou o melhor percentual de atividade antioxidante no ensaio do β-caroteno/ácido linoleico (41,12 ± 4,35%). Os extratos etanólico dos talos e metanólico das folhas mostraram-se ativos contra cepas de Bacillus cereus, Klebsiella pneumoniae, Staphylococcus aureus resistente à meticilina (MRSA), Staphylococcus aureus e Staphylococcus epidermidis. Termos para indexação: plantas medicinais, atemoia, atividade antibacteriana, radicais livres. ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF EXTRACTS FROM ATEMOIA (Annona cherimola Mill. x A. squamosa L.)ABSTRACT -The aim of this study was to quantify the levels of total phenols and flavonoids as well as to evaluate the antioxidant and antimicrobial activities of extracts obtained from stems and leaves of atemoia (A. cherimola Mill. x A. squamosa L.), which belongs to the Annonaceae family. The antioxidant activity was evaluated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) scavengers methods, as well as the method of co-oxidation of β-carotene/linoleic acid. The antimicrobial activity of the extracts was assessed against 10 strains of bacteria. The results showed that the antioxidant activity of the ethanolic extract from the stems (EEt) was the most effective antioxidant (IC 50 = 10.44 ± 1.25 mg/ml) by the DPPH method, as well as in the ABTS radical scavenger (24.81 ± 0.49%). The hexane extract of the leaves showed the best percentage of antioxidant activity by β-carotene/linoleic acid test (41.12 ± 4.35%). The ethanolic and metabolic extracts were active against strains of Bacillus cereus, Klebsiella pneumoniae, Methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus aureus and Staphylococcus epidermidis.

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In vitro and in vivo antioxidant effects of the ethanolic extract of Swertia chirayita

Cited by 110 publications

References 43 publications

Evaluation of antimicrobial and antioxidant activities of Swertia petiolata

Evaluation of antimicrobial and antioxidant activities of Swertia petiolata

Genetic diversity in Nepalese population of Swertia chirayita (Roxb. Ex Fleming) H. Karst based on inter-simple sequence repeats (ISSR) markers

Atividade antioxidante e antimicrobiana de extratos de atemoia (Annona cherimola Mill. x A. squamosa L.)

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