In Vitro and In Vivo Characterization of Pyocin

Abstract

Dcparozcni of .o\icrobiology, lfo(tYn Stotic ULnivcr.sitY School of' .1Icdicice, D)ctroit, Mlicligan 48207 Recci\ed clor publication 4 I-ebruar_\ 1967 Pyocin, a bacteriocin obtained from l1sates of ultraviolet-induced cultures of Pseuldcolionaces aceruiiginosa was clhLrLacterized in vitro and in vivo aLfter I,000-fold purification by chemical, column, and differential centrifugation procedures. Electron micrographs of negatively stained pyocin p)repalrations contaLined rod-shaped particles whichi resembled the contrcactile tcalil protein of the T-even phages of Escherichia coli. Although two septLrate anid distinct pyocin fractions were eluted from diethylamninoethyl cellulose (p1H 7.5) during the puriticatioll procedure, the pLar

Ribosomally encoded antibacterial proteins and peptides fromPseudomonas

“…Currently, five subgroups have been characterized, named R1-R5 (Michel-Briand & Baysse, 2002;Leiman & Shneider, 2012). Some additional R-type pyocins, not assigned to one of these groups, include C9 (Higerd et al, 1967), pyocin 21 (Govan, 1974a), and 430c (Govan, 1974b).…”

Defective pyocin particles produced by some mutant strains of Pseudomonas aeruginosa

“…R-type pyocins, a group of bacteriophage tail-like bacteriocins, have been found in many strains in Pseudomonas aeruginosa (4,8,9). They are classified into four groups (Rl, R2, R3, and R4) according to the difference in their receptor specificity, but they are morphologically indistinguishable from each other and are also very similar in their immunological properties (8).…”

Purification and Some Properties of Two Boticins

“…Interference of these bacteriocin-producing organisms with methods for the detection or recovery (or both) of toxin-producing type E has been demonstrated (25).The status of our knowledge regarding the bacteriocins has been reviewed frequently (3,9,10,17,21,30,34); the recent reviews of Reeves (34) and Bradley (3) are especially pertinent. Bradley's broad definition of bacteriocins as "bactericidal particles which are unable to multiply in a sensitive indicator" is applied in this report.Many bacteriocinogenic organisms elaborate maximal quantities of bacteriocins only after induction with ultraviolet light, metabolic inhibitors such as mitomycin C, or other agents known to induce lysogenic phages (2,10,13,18,20,24,34,37). Some organisms release bacteriocin continuously (11,26); in other cases, an abrupt release of bacteriocin coincides with partial or complete lysis of the culture (11,14,17,20,24).As Bradley (3) pointed out, the bacteriocins studied to date fall into two distinct categories.…”

“…Many bacteriocinogenic organisms elaborate maximal quantities of bacteriocins only after induction with ultraviolet light, metabolic inhibitors such as mitomycin C, or other agents known to induce lysogenic phages (2,10,13,18,20,24,34,37). Some organisms release bacteriocin continuously (11,26); in other cases, an abrupt release of bacteriocin coincides with partial or complete lysis of the culture (11,14,17,20,24).…”