In vitro and in vivo response of composites based on chitosan, hydroxyapatite and collagen

Abstract

The goal of this study was to evaluate the in vitro cellular toxicity and biological behavior of new bone graft composites after subcutaneous implantation during remodeling and wound-healing processes. We developed composites based on hydroxyapatite (obtained by deproteinizing bovine bone), collagen (obtained from bovine tendon) and chitosan (obtained from gladii of the squid species Loligo), that were characterized by different techniques (X-ray, FT-IR, Thermogravimetry, DSC and SEM). Three biomaterials were evaluated here: B1 (collagen/chitosan/hydroxyapatite), B2 (collagen/hydroxyapatite) B3 (collagen/hydroxyapatite). For in vitro cytotoxicity tests, two cell lines were used: HEp human larynx tumor cells (ATCC-CCL-23) and VERO cells from African green monkey (Cercopithecus aethiops). These toxicity tests demonstrated that the evaluated composites are not toxic. In biocompatibility tests, the results of a histological analysis showed that all three biomaterials present a low inflammatory tissue reaction. The tissue response was most favorable for sample B3, followed by B2 and B1, in that order. Based on these results, we conclude that all three biomaterials show good biocompatibility and no evidence of cytotoxicity; thus, these materials represent good candidates for tissue and graft engineering for use in bone regeneration.

Chitosan-collagen-hydroxyapatite membranes for tissue engineering

“…Composites with combinations of chitosan/hydroxyapatite/collagen scaffolds with 3D interconnected porosity have been very well studied for tissue regeneration, reporting good biocompatibility, bone defects restoration, and low cytotoxicity [46,[62][63][64][65][66]. However, there are scarce reports on membranes formed with these three compounds.…”

Avaliação in vitro do biomaterial compósito à base de hidroxiapatita, quitosana e nanotubo de carbono como adjuvante no reparo ósseo

“…Cell viability was assessed by MTT and PI assays using the Vero cell line (ATCC CCL-81) and MSCs derived from sheep bone marrow, which was performed in triplicate. Vero cells were chosen as the first approach based on the study by Amaral et al (2020) because they are easy to obtain and manipulate.…”

“…The viability of cultured cells was determined using the colorimetric MTT assay according to the manufacturer's protocol (Amaral et al, 2020). Two types of cells were used for this assay, the Vero cell line (ATCC CCL-81) and MSCs.…”

Chitosan/Silk Fibroin Materials for Biomedical Applications—A Review

“…In particular, collagen and chitosan blends are widely studied [ 7 , 8 , 9 , 10 , 11 , 12 ]. Using biopolymer blends, several new materials can be proposed for both biomedical and cosmetic applications ( Figure 1 ) [ 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ].…”