In vitro and in vivo evidence for the existence of presynaptic muscarinic cholinergic receptors in the rat hippocampus

Consolo, S.; Wang, Jian Xin; Fusi, R.; Vinci, Raffaella; Forloni, Gianluigi; Ladinsky, H.

doi:10.1016/0006-8993(84)91019-9

Cited by 16 publications

(4 citation statements)

References 18 publications

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“…Electrolytic lesion of the medial septum was used to remove cholinergic afferents to the hippocampus (17). The animals were allowed to recover for 7 days before transcardial perfusion as described above.…”

Section: Methodsmentioning

confidence: 99%

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Neuroanatomical localization and quantification of amyloid precursor protein mRNA by in situ hybridization in the brains of normal, aneuploid, and lesioned mice.

Bendotti

Forloni

Morgan

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

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Amyloid precursor protein mRNA was localized in frozen sections from normal and experimentally lesioned adult mouse brain and from normal and aneuploid fetal mouse brain by in situ hybridization with a M"S-labeled mouse cDNA probe. The MATERIALS AND METHODSTissue Preparation. Adult C57BL/6J and BALB/cJ mice were anesthetized with Avertin, then perfused transcardially with isotonic phosphate-buffered saline (PBS), and then immediately perfused with 4% (wt/vol) paraformaldehyde in PBS. The brains were removed, postfixed for 2 hr at 40C in fresh 4% (wt/vol) paraformaldehyde in PBS, and incubated overnight in 20% (wt/vol) sucrose in PBS for subsequent cryoprotection. The brains were then frozen rapidly in isopentane and dry ice. Ten-micrometer sections were cut with a cryostat, thaw-mounted onto slides coated with Denhardt's solution (0.02% polyvinylpyrrolidone/0.02% Ficoll/0.02% bovine serum albumin), and stored at -70°C.Adult BALB/cJ mice, anesthetized with halothane (3%) and mounted in a stereotactic apparatus, received unilateral injections of ibotenic acid (5 ikg in 0.5 1A of saline) into the hippocampal formation with a Hamilton microsyringe (16). Electrolytic lesion of the medial septum was used to remove cholinergic afferents to the hippocampus (17). The animals were allowed to recover for 7 days before transcardial perfusion as described above.The Tsl9 and Ts16 mouse fetuses were obtained by using a mating scheme described in detail in Gearhart et al. (18

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Section: Methodsmentioning

confidence: 99%

“…Sections were then rinsed for 1 hr in 1 x SSC at 550C and dehydrated. In situ hybridization was done in 20 ml of the same buffer plus 0.5 x 106 cpm of probe per section; the sections were covered with parafilm coverslips and incubated at 37°C for [16][17][18][19][20] hr.…”

Section: Methodsmentioning

confidence: 99%

Neuroanatomical localization and quantification of amyloid precursor protein mRNA by in situ hybridization in the brains of normal, aneuploid, and lesioned mice.

Bendotti

Forloni

Morgan

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

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“…This loss is accompanied by a consistent decrease of endogenous DA and of synaptosomal t3H]DA uptake in the same area. Only under some special circumstances (for instance, previous clearance of 50% of muscarinic receptors with intrahippocampal kainic acid before electrolytic lesion of the medial septa1 nucleus), could the presence of cholinergic autoreceptors located on homologous nerve terminals be demonstrated (Jerusalinsky et af., 1983 ;Consolo et al, 1984). In fact, the number of these presynaptic receptors, although sufficient to produce clear modulations of transmitter release in functional studies, is likely to be very small.…”

Section: Discussionmentioning

confidence: 99%

“…This is particularly true in the case of autoreceptors, since the innervated area often contains receptors of the same pharmacologic type, which are probably more numerous than the presynaptic autoreceptors and may become supersensitive after denervation (Westlind et al, 1981). Only under some special circumstances (for instance, previous clearance of 50% of muscarinic receptors with intrahippocampal kainic acid before electrolytic lesion of the medial septa1 nucleus), could the presence of cholinergic autoreceptors located on homologous nerve terminals be demonstrated (Jerusalinsky et af., 1983 ;Consolo et al, 1984). It is apparently easier to detect the lesion-induced disappearance of presynaptic heteroreceptors located on terminals that are particularly abundant in some areas, although even in this case results have rarely been very convincing.…”

Section: Discussionmentioning

confidence: 99%

[³]PIRENZEPINE BINDING IN RAT CORPUS STRIATUM DECREASES AFTER HEMITRANSECTION OF THE NIGRO‐STRIATAL PATHWAY

Pittaluga¹,

Versace

Marchi

et al. 1987

Fundamemntal Clinical Pharma

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The localization and pharmacologic characterization of muscarinic receptors possibly regulating the release of dopamine (DA) in rat corpus striatum were investigated by in vitro binding with [3H]pirenzepine ([3H]PZ) after hemitransection of the nigro-striatal pathway. DA levels in the corpus striatum ipsilateral to the lesion were substantially reduced by 66% compared with the unlesioned side after 8 days. The uptake of [3H]DA was also diminished by 63%. A significant decrease in the specific binding of [3H]PZ of 42% was seen in the corpus striatum ipsilateral to the lesion. The data indicate a loss of binding sites, whereas the lesion caused no change in the affinity constant for the muscarinic antagonist. The results support those previously obtained in studies of the muscarinic modulation of [3H]DA release from striatal synaptosomes and favor the idea that at least part of the muscarinic receptors regulating striatal DA release are localized on the nigro-striatal axon terminals and belong to the pirenzepine-sensitive subtype.

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Functional Organization of Dorsal Horn Interneurons

Willis

Coggeshall

2004

Sensory Mechanisms of the Spinal Cord

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In vitro and in vivo evidence for the existence of presynaptic muscarinic cholinergic receptors in the rat hippocampus

Cited by 16 publications

References 18 publications

Neuroanatomical localization and quantification of amyloid precursor protein mRNA by in situ hybridization in the brains of normal, aneuploid, and lesioned mice.

Neuroanatomical localization and quantification of amyloid precursor protein mRNA by in situ hybridization in the brains of normal, aneuploid, and lesioned mice.

[³]PIRENZEPINE BINDING IN RAT CORPUS STRIATUM DECREASES AFTER HEMITRANSECTION OF THE NIGRO‐STRIATAL PATHWAY

Functional Organization of Dorsal Horn Interneurons

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In vitro and in vivo evidence for the existence of presynaptic muscarinic cholinergic receptors in the rat hippocampus

Cited by 16 publications

References 18 publications

Neuroanatomical localization and quantification of amyloid precursor protein mRNA by in situ hybridization in the brains of normal, aneuploid, and lesioned mice.

Neuroanatomical localization and quantification of amyloid precursor protein mRNA by in situ hybridization in the brains of normal, aneuploid, and lesioned mice.

[3]PIRENZEPINE BINDING IN RAT CORPUS STRIATUM DECREASES AFTER HEMITRANSECTION OF THE NIGRO‐STRIATAL PATHWAY

Functional Organization of Dorsal Horn Interneurons

Contact Info

Product

Resources

About

[³]PIRENZEPINE BINDING IN RAT CORPUS STRIATUM DECREASES AFTER HEMITRANSECTION OF THE NIGRO‐STRIATAL PATHWAY