In vitro and in vivo Anti-arthritic and Antioxidant potential of Glycosmis pentaphylla in Complete Freund’s Adjuvant model

Abstract

Rheumatoid arthritis (RA) is a systemic, chronic inflammatory and auto immune disorder. RA is provoked by genetical predisposition and environmental factors. Oxidative stress makes the human liable to diseases like diabetes, RA, cancer, atherosclerosis etc. Hence the study investigated the anti-arthritic and oxidative activity of ethanolic extract of Glycosmis pentaphylla (EEGP) as it has not been pharmacologically tested. Anti-arthritic potential was evaluated in vitro using protein denaturation by bovine serum albumin and in vivo by Complete Freund Adjuvant (CFA) models at 200 and 400mg/kg doses. Also, in vitro and in vivo antioxidant ability was appraised by DPPH scavenging and assessment of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and lipid peroxidation (LPO). The results exhibited concentration dependent inhibition of albumin denaturation with maximum results obtained at 800μg/ml. The results of CFA model depicted better protection against arthritic lesions and body weight alterations. Also G. pentaphylla remarkably ameliorated histopathological changes. Additionally, plant exhibited remarkable anti-oxidant activity.