In vitro and in vivo biocompatibility of decellularized cellulose scaffolds functionalized with chitosan and platelet rich plasma for tissue engineering applications

Mahendiran, Balaji; Muthusamy, Shalini; Sampath, S.; Jaisankar, Sellamuthu N.; Selvakumar, R.; Krishnakumar, Gopal Shankar

doi:10.1016/j.ijbiomac.2022.07.052

Cited by 7 publications

(1 citation statement)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CS was chosen to be a component of the collagen film due to its excellent characteristics and broad applications (Andonegi et al 2020;Heras et al 2022;Mahendiran et al 2022;Valenzuela-Rojo et al 2020) (Fig. 5a).…”

Section: Collagen Film Characteristicsmentioning

confidence: 99%

Expression, characterization, and application potentiality evaluation of recombinant human-like collagen in Pichia pastoris

Liao

et al. 2022

Bioresour. Bioprocess.

View full text Add to dashboard Cite

Collagen is a biofunctional protein that has been widely used in many fields, including but not limited to biomedical, cosmetics and skin care, food, and novel materials. Recombinant collagen has great potential as an alternative to collagen extracted from animals because it avoids the immune response, and the yield and properties are stable. However, challenges remain in the industrial application of recombinant collagen, including improving the expression yield, reducing the cost of purification for industry and expanding applications. In this study, a cloning and recombination method was used to heterologously express the recombinant human-like collagen (RHLC) in Pichia pastoris GS115 using the pPIC9k expression vector. The RHLC expression titre was 2.33 g/L via a 5-L fermenter, and the purification was completed within 48 h and was 98% pure. The characteristics of RHLC were investigated. Furthermore, potential applications for RHLC were explored, such as basal collagen sponge preparation, forming films with chitosan and production of collagen hydrolysed peptides. RHLC has various potential applications due to its triple helical structure, thermostability, good biocompatibility and film-forming ability. Graphical Abstract

show abstract

Section: Collagen Film Characteristicsmentioning

confidence: 99%

Expression, characterization, and application potentiality evaluation of recombinant human-like collagen in Pichia pastoris

Liao

et al. 2022

Bioresour. Bioprocess.

View full text Add to dashboard Cite

show abstract

Cellulose‐enriched ascorbic acid for wound dressing application: Future medical textile

Firmanda,

Mahardika,

Fahma

et al. 2024

J of Applied Polymer Sci

View full text Add to dashboard Cite

Wounds infected by resistant microbes have become a cost and health problem worldwide. In modern wound care, healing mechanisms require particular strategies, such as adding natural antioxidants and antimicrobials in a biocompatible biopolymer matrix mimicking extracellular tissue such as cellulose. Here, ascorbic acid or Vitamin C is a promising bioactive as a topical drug loaded in a cellulose‐based composite matrix for skin tissue repair. Ascorbic acid, cellulose, or cellulose composites enriched with ascorbic acid have shown better biocompatibility and biological effects for accelerating wound healing, making them promising as sustainable medical textiles. Future challenges relate to the ideal engineered wound dressing design, raw material toxicity, and 3D printing technology.

show abstract

Plant-derived biomaterials and scaffolds

et al. 2023

View full text Add to dashboard Cite

In vitro and in vivo biocompatibility of decellularized cellulose scaffolds functionalized with chitosan and platelet rich plasma for tissue engineering applications

Cited by 7 publications

References 49 publications

Expression, characterization, and application potentiality evaluation of recombinant human-like collagen in Pichia pastoris

Expression, characterization, and application potentiality evaluation of recombinant human-like collagen in Pichia pastoris

Cellulose‐enriched ascorbic acid for wound dressing application: Future medical textile

Plant-derived biomaterials and scaffolds

Contact Info

Product

Resources

About