In-vitro and in-vivo antioxidant activity of different extracts of the leaves of <i>Clerodendron colebrookianum</i> Walp in the rat

Rajlakshmi, Devi; Banerjee, Sanjay K.; Sk, Sood; Maulik, Subir Kumar

doi:10.1211/0022357022296

Cited by 29 publications

(12 citation statements)

References 22 publications

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“…Heart was excised, washed with 0.9% NaCl solution and homogenised with 20-times volume of heart weight in 0.05 M ice-cold phosphate buffer [pH 7.4] [13]. Heart homogenate (0.25 ml) was mixed with 0.1 ml of 0.05 M phosphate buffer (pH 7.4), 0.05 ml of 0.1 mM ascorbic acid, 0.05 ml of 4 mM FeCl2 solution and 0.05 ml of the test sample.…”

Section: Methodsmentioning

confidence: 99%

Cardioprotective effect of ritonavir, an antiviral drug, in isoproterenol induced myocardial necrosis: a new therapeutic implication

et al. 2013

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BackgroundRitonavir is a HIV protease inhibitor. In addition to its antiviral effect, Ritonavir directly inhibits the insulin-regulated glucose transporter GLUT4 and blocks glucose entry into fat and muscle cells. However, the effect of Ritonavir on cardiac GLUT4 inhibition during myocardial necrosis is not investigated. In the present study, we evaluated the role of Ritonavir in isoproterenol-induced myocardial necrosis in vivo and compared the effect with Phlorizin, a nonslective SGLTs inhibitor.MethodsIsoproterenol (ISO) (150 mg/kg/day, i.p for 2 consecutive days) was administered to mice to cause myocardial necrosis. Phlorizin (400 mg/kg/day i.p twice daily for 2 days) and Ritonavir (10 mg/kg/day i.p twice daily for 2 days) were administered in two different groups of mice before isoproterenol administration.Results and discussionIsoproterenol (ISO) (150 mg/kg/day, i.p for 2 consecutive days) administration caused significant (p < 0.05) increase in heart/body weight ratio, and myocardial necrosis as evident by significant (p < 0.05) increase in serum markers i.e. SGOT and CK; and cardiac histopathological changes. Significant (p < 0.05) reduction in myocardial SOD and catalase activities, and GSH level along with a significant (p < 0.05) rise in myocardial TBARS and nitric oxide levels were observed after ISO administration. However, administration of phlorizin, a SGLT1 inhibitor has been found to exhibit partial protection in ISO induced myocardial necrosis, as observed by significant decrease in heart/body weight ratio and myocardial nitric oxide level; significant increase in myocardial SOD and catalase activities along with no histopathological alterations. On the other hand, administration of ritonavir, a nonspecific GLUT inhibitor has been found to exhibit complete protection as observed by normalisation of heart/body weight ratio, serum markers, antioxidant enzymes activities and histopathological alterations. In vitro study with heart homogenate confirmed no antioxidant effect of ritonavir and phlorizin in the absence and presence of isoproterenol.ConclusionsOur study concluded that ritonavir, a nonspecific GLUT inhibitors showed complete protection in catecholamine induced myocardial necrosis.

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Section: Methodsmentioning

confidence: 99%

Cardioprotective effect of ritonavir, an antiviral drug, in isoproterenol induced myocardial necrosis: a new therapeutic implication

et al. 2013

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“…Nutritional antioxidant deficiency also leads to oxidative stress which signifies the identification of natural antioxidative agents present in die consumed by human population (Hogg et al, 1961;Cao et al, 1966). Plant extracts rich in phenol acids exhibit strong antioxidant and antiradical activity in vitro (Mary et al, 2003) and in vivo (Rajlakshmi et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant compounds, assays of determination and mode of action

Shalaby¹,

Shanab²

2013

Afr. J. Pharm. Pharmacol.

115

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The human body uses an antioxidant defense system to neutralize the excessive levels of reactive oxygen species. This system consists of enzymatic and non enzymatic antioxidants, catalase, peroxidase, superoxide dismutase and gluatathion s-transferease as major defense enzymes. However, ascorbic acid, tocopherol, and phenolic compounds are considered as examples for non-enzymatic antioxidants. Increasing research on natural antioxidants in foods and development of new assays has prompted critical reflection on the field. It has been common practice to identify health benefits from antioxidant activity on the cellular level with antioxidant capacity of food measured in vitro. The use of antioxidants and their positive effects on food quality has been demonstrated in a large variety of foods and beverages using various methods for detection of lipid and protein oxidation or various assays based on electron transfer or hydrogen-atom transfer. There is a need for screening studies in order to identify the mode of action of different antioxidant compounds (enzymatic and non-enzymatic in addition, comparing between synthetic and natural antioxidant compounds) by different assays, in addition to highlighting the advantage and disadvantage of it. Some of these assays depend on hydrogen atom transfer methods or electron transfer methods in addition, metal chelating compounds and free radical scavenging activity.

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“…The heart, liver and kidney were removed, washed in cold saline and stored in liquid nitrogen for further biochemical studies. This in vivo antioxidant activity was analyzed by the method described by Rajlakshmi et al [26].…”

Section: Test Animals and Groupsmentioning

confidence: 99%

In Vivo Antioxidant Activity of Limnophila Heterophylla and Michelia Champaca

Raja

Koduru

2017

Int J Pharm Pharm Sci

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Objective: The present study was aimed at investigating the in-vivo antioxidant activity of the methanol extracts of Limnophila heterophylla and Michelia champaca leaves.Methods: Methanol extract of both plants were administered to rats separately at three different doses of 125, 250 and 500 mg/kg for 21 d to evaluate oxidative stress parameters such as ferric reducing ability of plasma (FRAP), thiobarbituric acid reactive substance (TBARS) and reduced glutathione (GSH) and to evaluate antioxidant enzyme levels of catalase (CAT) and superoxide dismutase (SOD). Results:The methanol extracts of both the plants significantly (p<0.05) elevated the ferric reducing ability of plasma (FRAP) on days 7, 14 and 21 of treatment. Significant (p<0.05) decrease of thiobarbituric acid reactive substance (TBARS) levels along with an increase in the superoxide dismutase (SOD) enzyme level in the liver and kidney at three different doses both the plants was observed. Treatment at a dose of 500 mg/kg b. w of both plants caused a significant increase only in the level of CAT in the liver and kidney. However, there was no significant effect of a thiobarbituric acid reactive substance (TBARS), superoxide dismutase (SOD) and catalase (CAT) in the heart and reduced glutathione (GSH) level in liver, heart and kidney at three different doses both the plants. Conclusion:These outcomes recommend that the leaves of Limnophila heterophylla and Michelia champaca have a potent antioxidant activity which may be responsible for some of its reported pharmacological actions.

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In-vitro and in-vivo antioxidant activity of different extracts of the leaves of Clerodendron colebrookianum Walp in the rat

Cited by 29 publications

References 22 publications

Cardioprotective effect of ritonavir, an antiviral drug, in isoproterenol induced myocardial necrosis: a new therapeutic implication

Cardioprotective effect of ritonavir, an antiviral drug, in isoproterenol induced myocardial necrosis: a new therapeutic implication

Antioxidant compounds, assays of determination and mode of action

In Vivo Antioxidant Activity of Limnophila Heterophylla and Michelia Champaca

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