In-vitro and In-vivo Anticancer Activity of Indigofera cordifolia against Ehrlich Ascites Carcinoma

Abstract

The research study investigated the anticancer properties of Indigofera cordifolia in relation to ehrlich ascites carcinoma (EAC) in mice. The substance was administered intraperitoneally at 10 mg per kilogram of body‑weight. The extract was supplied nine successive days and mice were euthanized after 24 hours since the previous dosage and after fasting for 18 hours. The antitumor impact was evaluated by measuring tumor dimensions, counting viable & non-viable cancer cells, measuring the weight of tumor, & analyzing hematological considerations of the host harboring EAC. A histopathological examination of the liver sections was conducted. The study found a statistically considerable (p < 0.001) improvement in endurance times for tumor-bearing mice treated with ethanolic extraction of I. cordifolia. The extract also reduced the weight of subjects with EAC tumors. Hematological analyses indicate a reduction in hemoglobin (Hb) levels in mice treated with EAC, while extract-treated animals showed a restoration of Hb levels close to normal. Noted with a statistically significant decrease (p <0.001) in red blood cell count up & boost in white blood cell count in mice supplied with the extract/fraction compared to those treated with EAC. The study stated that extract had noteworthy anticancer activity, which was equivalent to that of doxorubicin. The histological sections of the liver tissue showed enhanced hepatic architecture following treatment with a 400 mg/kg dosage of the test extract.