In vitro and in vivo antibacterial activity of tigecycline against Vibrio vulnificus

Tang, Hung Jen; Chen, Chi Chung; Lai, Chih Cheng; Zhang, Chun Cheng; Weng, Tzu Chieh; Chiu, Yu Hsin; Toh, Han Siong; Chiang, Shyh Ren; Yu, Wen Liang; Ko, Wen Chien; Chuang, Yin Ching

doi:10.1016/j.jmii.2016.04.009

Cited by 8 publications

(7 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In previous antimicrobial susceptibility studies using MIC testing, the susceptibility rate of E. anophelis to tigecycline ranged from 5.1% to 26.4% [7]. Although tigecycline is mainly considered to be a bacteriostatic antimicrobial agent [23], this new glycylcycline antibiotic exhibits bactericidal activity against some bacterial species, including Klebsiella pneumoniae, Acinetobacter baumannii, and Vibrio vulnificus [24][25][26]. However, our study revealed that tigecycline possessed only bacteriostatic activity against E. anophelis but no bactericidal effect, even at 4 × MIC.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Effects of Minocycline, Tigecycline, Ciprofloxacin, and Levofloxacin against Elizabethkingia anophelis Using In Vitro Time-Kill Assays and In Vivo Zebrafish Animal Models

et al. 2021

View full text Add to dashboard Cite

Elizabethkingia anophelis is a multidrug-resistant pathogen. This study evaluated the antimicrobial activity of minocycline, tigecycline, ciprofloxacin, and levofloxacin using in vitro time-kill assays and in vivo zebrafish animal models. The E. anophelis strain ED853-49 was arbitrarily selected from a bacterial collection which was concomitantly susceptible to minocycline, tigecycline, ciprofloxacin, and levofloxacin. The antibacterial activities of single agents at 0.5–4 × minimum inhibitory concentration (MIC) and dual-agent combinations at 2 × MIC using time-kill assays were investigated. The therapeutic effects of antibiotics in E. anophelis-infected zebrafish were examined. Both minocycline and tigecycline demonstrated bacteriostatic effects but no bactericidal effect. Minocycline at concentrations ≥2 × MIC and tigecycline at concentrations ≥3 × MIC exhibited a long-standing inhibitory effect for 48 h. Bactericidal effects were observed at ciprofloxacin and levofloxacin concentrations of ≥3 × MIC within 24 h of initial inoculation. Rapid regrowth of E. anophelis occurred after the initial killing phase when ciprofloxacin was used, regardless of the concentration. Levofloxacin treatment at the concentration of ≥2 × MIC consistently resulted in the long-lasting and sustainable inhibition of bacterial growth for 48 h. The addition of minocycline or tigecycline weakened the killing effect of fluoroquinolones during the first 10 h. The minocycline-ciprofloxacin or minocycline–levofloxacin combinations achieved the lowest colony-forming unit counts at 48 h. Zebrafish treated with minocycline or a combination of minocycline and levofloxacin had the highest survival rate (70%). The results of these in vitro and in vivo studies suggest that the combination of minocycline and levofloxacin is the most effective therapy approach for E. anophelis infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Antimicrobial Effects of Minocycline, Tigecycline, Ciprofloxacin, and Levofloxacin against Elizabethkingia anophelis Using In Vitro Time-Kill Assays and In Vivo Zebrafish Animal Models

et al. 2021

View full text Add to dashboard Cite

show abstract

“…18,19 In refractory cases, tigecycline has been reportedly used as an alternative salvage agent. 5,20,21 With global warming and pollution disrupting the marine microbiome, V. vulnificus incidence rates will continue to rise. For the clinician, obtaining a thorough epidemiological history will avoid unnecessary antibiotic delays.…”

Section: Discussionmentioning

confidence: 99%

Late-onsetVibrio vulnificussepticemia without cirrhosis

Lee

Dinh

Nguyen

et al. 2019

Baylor University Medical Center Proceedings

View full text Add to dashboard Cite

Recent surveillance from the Centers for Disease Control and Prevention indicates rising annual incidence rates of Vibrio vulnificus infection. Unfortunately, this infection is often excluded from the differential diagnosis in lesser known at-risk populations. Transmission occurs via wound exposure or ingestion, with V. vulnificus foodborne illness having the highest mortality rate of all Vibrio species. Fatality rates of V. vulnificus rival those of Ebola and bubonic plague, so timely treatment is imperative. Current literature favors surgical debridement with a third-generation cephalosporin plus intravenous doxycycline or fluoroquinolone. Cephalosporin monotherapy is discouraged due to rising resistance. This case features V. vulnificus septicemia with prolonged incubation time in a noncirrhotic patient.

show abstract

“…The in vitro data from the present study demonstrated that the antibacterial effect of TGC alone was more distinctive than that of the DOX/CAZ combination (p = 0.028). Tang et al (Tang et al, 2016) previously reported that TGC monotherapy has a similar killing effect as the combination of minocycline and cefotaxime in experimental murine infection with V. vulnificus. In addition, a recent study (Lin et al, 2016) showed that patient who received TGC had a significantly favorable microbiological response and clinical outcomes, which is similar to one of the cases reported in the present study.…”

Section: Tablementioning

confidence: 91%

Clinical features and treatment of patients with Vibrio vulnificus infection

Shen

Pan

et al. 2017

International Journal of Infectious Diseases

View full text Add to dashboard Cite

show abstract

In vitro and in vivo antibacterial activity of tigecycline against Vibrio vulnificus

Abstract: Our in vitro combination and animal studies indicate that tigecycline could be an option for the treatment of invasive V. vulnificus infections.

Cited by 8 publications

References 27 publications

Antimicrobial Effects of Minocycline, Tigecycline, Ciprofloxacin, and Levofloxacin against Elizabethkingia anophelis Using In Vitro Time-Kill Assays and In Vivo Zebrafish Animal Models

Antimicrobial Effects of Minocycline, Tigecycline, Ciprofloxacin, and Levofloxacin against Elizabethkingia anophelis Using In Vitro Time-Kill Assays and In Vivo Zebrafish Animal Models

Late-onsetVibrio vulnificussepticemia without cirrhosis

Clinical features and treatment of patients with Vibrio vulnificus infection

Contact Info

Product

Resources

About