In vitro and in vivo study of glutamate dehydrogenase encapsulated into mouse erythrocytes by a hypotonic dialysis procedure

Sanz, Sílvia; Lizano, Carmen; Luque, J.; Pinilla, Montserrat

doi:10.1016/s0024-3205(99)00546-9

Cited by 40 publications

(39 citation statements)

References 22 publications

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“…Again, some cell-protecting effect of hydrogel structures becomes evident. In other studies, 27,29,30 all these parameters were decreased in carrier erythrocytes compared with the normal unloaded cells. From the size distribution of the erythrocytes represented by the data in Table 5: first, the erythrocytes showed normal unimodal distribution in all four groups; second, the sizes correspond the mean equivalent diameters of normal erythrocytes; third, encapsulation of nanoparticle-loaded valproate in erythrocytes has no significant effect on cell sizes; and fourth, the encapsulation procedure alone exerts no changes in cell sizes.…”

Section: In Vitro Characterization Of Carrier Erythrocytesmentioning

confidence: 61%

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Encapsulation of Valproate-Loaded Hydrogel Nanoparticles in Intact Human Erythrocytes: A Novel Nano-cell Composite for Drug Delivery

Hamidi

Rafiei

Azadi

et al. 2011

Journal of Pharmaceutical Sciences

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Section: In Vitro Characterization Of Carrier Erythrocytesmentioning

confidence: 61%

“…27,28 Turbulence fragility curves of nanoparticles-loaded erythrocytes (Fig. 6) compared with the normal unloaded cells indicate a remarkable increase in cell resistance against the vigorous mechanical stresses even after 20 h of shaking.…”

Section: In Vitro Characterization Of Carrier Erythrocytesmentioning

confidence: 97%

Encapsulation of Valproate-Loaded Hydrogel Nanoparticles in Intact Human Erythrocytes: A Novel Nano-cell Composite for Drug Delivery

Hamidi

Rafiei

Azadi

et al. 2011

Journal of Pharmaceutical Sciences

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“…Erythrocytes were washed in 150 mM NaCl. Hypotonized erythrocytes were obtained through previously reported technique (17). Briefly, 0.5 ml of packed erythrocytes (70% hematocrit) were placed in a dialysis bag (Medicell, molecular size cutoff, 12-14 kDa) and dialyzed against 50 volumes of hypotonic (40 mOsm/kg) phosphate-buffered saline (PBS) (15 mM NaHCO 3 , 15 mM NaH 2 PO 4 , 20 mM glucose, 4 mM MgCl 2 , pH 7.4 plus 2 mM ATP and 3 mM reduced glutathione) under gentle rotation at 4…”

Section: Hypotonic-isotonic Dialysis Of Mouse Erythrocytesmentioning

confidence: 99%

“…This characteristic feature has allowed employing erythrocytes as suitable carriers for drugs and other pharmacological agents deliveries to the MPS. The use of differently modified carrier erythrocytes as a system for targeting encapsulated compounds to specific organs (mainly the liver) was already studied extensively in our laboratory (14,15,17,25). Thus, it was demonstrated that the presence or absence of a drug in the erythrocytes does not modify the cell's basic behaviour (28).…”

Section: In Vivo Biodistribution Of Tf-erythrocytesmentioning

confidence: 99%

“…This method allowed encapsulating high percentage of the chosen drug and, at the same time, rendered high percentages of cell recovery and long survival rates, once the carrier erythrocytes have been reinjected into experimental animals (11)(12)(13)(14)(15). Our group's previous experimentations with the hypotonic-isotonic dialysis method have demonstrated the effective encapsulation of carbonic anhydrase, recombinant human, erythropoietin, L-asparaginase, and several dehydrogenases in rat, human, or mouse erythrocytes (14)(15)(16)(17). In the current experiments, Tf was linked to hypotonized erythrocytes (Tf-erythrocytes) using glutaraldehyde (GTA) as a coupling agent.…”

Section: Introductionmentioning

confidence: 95%

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In Vivo Studies on Mouse Erythrocytes Linked to Transferrin

et al. 2002

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SummaryTransferrin (Tf), a plasma protein with numerous, highly specific receptors in proliferating and differentiating cells was already discussed as a targeting ligand for drugs and liposomes in previous studies. In this paper, we deal with erythrocytes linked to Tf as possible physiological targeting carrier systems for delivering anticancer drugs. For that purpose we have used glutaraldehyde (0.1%) as a coupling agent between Tf and erythrocytes. The highest amount of Tf linked to erythrocytes turned out to be 0.76 ± 0.13 µg Tf/10 6 cells, while reaching 65% of cell recovery. After 13 days, the Tf-erythrocytes hemolysis reached 50%, with transferrin still coupled to erythrocytes. The in vivo kinetic behaviour of intravenously injected 51 Cr-Tf-erythrocytes showed a reduced half-life to hours as compared to days of controls. However, a considerable percentage of Tf-erythrocytes (close to 20%) remained circulating for a relatively long period (around 2 days), which made possible the specific targeting by these carrier systems. In vivo biodistribution studies indicated that 51 Cr-Tf-erythrocytes rapidly accumulated in the different studied organs (liver, spleen, lungs, kidneys, femur-tibia, and heart), suggesting a selective removal of Tf-erythrocytes by the cells of the mononuclear phagocytic system present mainly in liver and spleen. On the other hand, Tf-erythrocytes showed a poor targeting of heart tissue, therefore a reduced cardiac toxicity should be expected after administration of erythrocyte-encapsulated drugs. The presence of Tf-erythrocytes in femur-tibia and spleen could be related to the Tf-specific binding to the hematopoietic cells containing Tf receptors. The final results of this study encourage additional research on Tf-erythrocyte to investigate the relationship between transferrin-mediated targeting by carrier erythrocytes and uptake of different erythrocyte-encapsulated drugs. Consequently, the current study showed possible use of these carriers as a potential therapeutic tool for drug targeting in animal models with alterations affecting mononuclear phagocytic system or carcinomas of various origins whose cells show elevated number of Tf receptors.

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Drug delivery by red blood cells

et al. 2011

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SummaryDrug delivery is a growing field of interdisciplinary activities that combine the use of new materials with the biochemical properties of selected drugs, with the aim of improving their therapeutic action and reducing their toxicity. In few cases, proper medical devices have been also realized to implement new drug delivery modalities. In this article, we have summarized available information and our experience on the use of autologous Red Blood Cells as carriers for drugs to be released within the vascular system. This is not a comprehensive review, but it focusses on the mechanisms that are available to distribute drugs in circulation by carrier red blood cells and provide illustrative examples on how this is currently obtained. We have not included a summary of clinical data collected in recent years using this technology but simply provided proper references for the interested readers. Finally, a special attention is devoted to the possibility of entrapping, into autologous red blood cells, recombinant drug-binding proteins. This new strategy is opening the way at a new modality to influence the vascular distribution of drugs by realizing a dynamic circulating container (the engineered red cell) capable of reversible binding and transportation of one or more drugs of interest selected on the bases of the red cell entrapped target proteins. This new modality is not yet fully developed and explored but will certainly provide a technical solution to the problem of stabilizing drug concentration in circulation improving drug efficacy and reducing drug toxicity.

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In vitro and in vivo study of glutamate dehydrogenase encapsulated into mouse erythrocytes by a hypotonic dialysis procedure

Cited by 40 publications

References 22 publications

Encapsulation of Valproate-Loaded Hydrogel Nanoparticles in Intact Human Erythrocytes: A Novel Nano-cell Composite for Drug Delivery

Encapsulation of Valproate-Loaded Hydrogel Nanoparticles in Intact Human Erythrocytes: A Novel Nano-cell Composite for Drug Delivery

In Vivo Studies on Mouse Erythrocytes Linked to Transferrin

Drug delivery by red blood cells

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