In vitro and in vivo anti-inflammatory and anti-arthritic effect of Tinospora cordifolia via modulation of JAK/STAT pathway

George, Genu; Shyni, G. L.; Mohan, Sreelekshmi; Abraham, Billu; Nisha, P.; Sreedharan, Ranjith; Rajankutty, K; Raghu, K.

doi:10.1007/s10787-023-01155-7

Cited by 5 publications

(9 citation statements)

References 58 publications

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“…Hook. f. and Thomson also showed anti-inflammatory effects on lypossacharide-stimulated RAW 264.7 cells by reducing IL-6, TNF-α, PGE2, and nitric oxide levels and iNOS and COX expression via modulation of the JAK/STAT pathway [317]. On the other hand, 100-200 µM blueberry polyphenols can reduce TNF-α, IL-1β, MMP3, and NF-κB levels on HIG-82 rabbit synoviocytes previous stimulated with TNF-α [318].…”

Section: Clinical Trialsmentioning

confidence: 89%

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Potential Role of Dietary Phenolic Compounds in the Prevention and Treatment of Rheumatoid Arthritis: Current Reports

Gonçalves,

Rodrigues,

Fonseca

et al. 2024

Pharmaceuticals

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Rheumatoid arthritis (RA) is a complex illness with both hereditary and environmental components. Globally, in 2019, 18 million people had RA. RA is characterized by persistent inflammation of the synovial membrane that lines the joints, cartilage loss, and bone erosion. Phenolic molecules are the most prevalent secondary metabolites in plants, with a diverse spectrum of biological actions that benefit functional meals and nutraceuticals. These compounds have received a lot of attention recently because they have antioxidant, anti-inflammatory, immunomodulatory, and anti-rheumatoid activity by modulating tumor necrosis factor, mitogen-activated protein kinase, nuclear factor kappa-light-chain-enhancer of activated B cells, and c-Jun N-terminal kinases, as well as other preventative properties. This article discusses dietary polyphenols, their pharmacological properties, and innovative delivery technologies for the treatment of RA, with a focus on their possible biological activities. Nonetheless, commercialization of polyphenols may be achievable only after confirming their safety profile and completing successful clinical trials.

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Section: Clinical Trialsmentioning

confidence: 89%

“…Hook. f. and Thomson reduced erythema, paw edema, hyperplasia, IL-6, TNF-α, IL-17, nitric oxide, and PGE2 levels, as well as phosphorylation of STAT3 and the expression of VEGF [317].…”

Section: In Vivo Studiesmentioning

confidence: 92%

Potential Role of Dietary Phenolic Compounds in the Prevention and Treatment of Rheumatoid Arthritis: Current Reports

Gonçalves,

Rodrigues,

Fonseca

et al. 2024

Pharmaceuticals

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“…ex Maton ethanol extracts have immunomodulatory effects of increasing NO, TNF-α, IL-6, and COX-2 production. 25,35 This study showed that Boesenbergia rotunda (L.) Mansf, Curcuma aeruginosa Roxb., Curcuma zanthorrhiza Roxb., and Zingiber officinale var. Rubra plays a role in reducing NO production in LPS-induced RAW 264.7 cells.…”

Section: Activation Application Of Raw 2647 In Zingiberaceaementioning

confidence: 97%

“…The major adaptor protein of TLR4 is myeloid differentiation primary response gene 88 (MyD88), which leads to downstream signaling. 25 The hydrophobic pocket of myeloid differentiation protein 2 (MD2) directly binds to five lipid chains of LPS associated with TLR4. The homodimer of the TLR4-MD2 complex on the cell surface then recruits central adaptor proteins such as MyD88, which is linked to IL-1 receptor-associated kinase (IRAK)4.…”

Section: Signaling Pathways In Macrophage Activationmentioning

confidence: 99%

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RAW 264.7 Macrophage Cell Line: In Vitro Model for the Evaluation of the Immunomodulatory Activity of Zingiberaceae

2023

TJNPR

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The immune response plays an essential role in the body's defense against infection. Macrophages are promising targets against which to screen agents that modulate immune responses. In vitro analysis, primarily using cell lines, is preferred to screen bioactivity initially. RAW 264.7 cells comprise a model macrophage cell line close to primary murine macrophages. Indonesian people often use the Zingiberaceae family or “empon-empon" in the favorable treatment of several diseases, including immune disorders. This review highlights several studies of Zingiberaceae using RAW 264.7 cells, focusing on the observed immunomodulatory activity and assay methods. The research on RAW 264.7 macrophage cell line in Zingiberaceae was gathered using Scopus, PubMed, ScienceDirect, and Google Scholar from 2012-2021. Based on the reviews, the Zingiberaceae family has immunomodulatory effects by increasing or decreasing inflammatory mediators such as NO, IL-6, TNF-α, IL-1β, IL-10, and phagocytosis activity in RAW 264.7 cells. Several methods can assess the activation of RAW 264.7 cells, including cell viability assays using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT); measuring nitric oxide production (Griess reaction), cytokine production (enzyme-linked immunosorbent assay [ELISA]), and phagocytosis (neutral red uptake assay); and detecting DNA (real-time quantitative polymerase chain reaction [qPCR]). The information provided in this review presents avenues for future investigations of the immunomodulatory activity of Zingiberaceae.

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Evaluating the Efficacy of a Polyherbal Formulation in Ameliorating Arthritis Induced by Complete Freund’s Adjuvant

Chitra,

Damodharan,

Narasimha

et al. 2024

JNR

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Background: This study investigates the antiarthritic potential of a polyherbal formulation (PHF) comprising extracts from Tinospora cordifolia, Rosa damescena, and Acacia leucoploea in a rat model of rheumatoid arthritis (RA). RA is a chronic autoimmune disorder with debilitating consequences. PHF's impact on joint inflammation, bone degradation, and cartilage preservation were evaluated. Methods: Arthritis was induced using complete Freund’s adjuvant (CFA), and animals were treated with PHF (200 and 400 mg/kg), prednisolone, or control treatments for 28 days. Parameters including body weight, paw volume, arthritis severity score, hematological parameters, serum markers (creatinine, ALP, total proteins), cytokine levels (IL-1β, IL-6, TNF-α, IL-10), and radiographic changes were assessed. Results: CFA-treated rats exhibited significant body weight loss, paw edema, increased arthritis severity scores, altered hematological parameters, and elevated serum markers compared to normal controls. PHF treatment at both doses mitigated body weight loss, reduced paw edema, and improved arthritis severity scores. Hematological changes induced by CFA were also attenuated by PHF treatment. Serum creatinine, ALP, and total protein levels, elevated in CFA-treated rats, were significantly improved by PHF. Furthermore, PHF modulated cytokine levels, decreasing IL-6, IL-1β, and TNF-α while increasing IL-10. Radiographic analysis displayed reduced joint damage in PHF-treated rats compared to CFA controls. Conclusion: This comprehensive investigation highlights PHF's potential to mitigate the inflammatory processes associated with RA, as evidenced by improved clinical, hematological, and biochemical parameters. The study underscores the promise of traditional botanical compounds in managing RA and suggests PHFs as novel therapeutic options. Further mechanistic studies are warranted to elucidate the exact pathways involved.

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In vitro and in vivo anti-inflammatory and anti-arthritic effect of Tinospora cordifolia via modulation of JAK/STAT pathway

Cited by 5 publications

References 58 publications

Potential Role of Dietary Phenolic Compounds in the Prevention and Treatment of Rheumatoid Arthritis: Current Reports

Potential Role of Dietary Phenolic Compounds in the Prevention and Treatment of Rheumatoid Arthritis: Current Reports

RAW 264.7 Macrophage Cell Line: In Vitro Model for the Evaluation of the Immunomodulatory Activity of Zingiberaceae

Evaluating the Efficacy of a Polyherbal Formulation in Ameliorating Arthritis Induced by Complete Freund’s Adjuvant

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