In vitro and in vivo antimicrobial activity of antimicrobial peptide Jelleine-I against foodborne pathogen Listeria monocytogenes

Shen, Panpan; Ding, Kang; Wang, Lifang; Tian, Jie; Huang, Xiuhong; Zhang, Mingyu; Dang, Xiangli

doi:10.1016/j.ijfoodmicro.2022.110050

Cited by 15 publications

(16 citation statements)

References 39 publications

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“…Genomic DNA was extracted from Psa cells using a genomic DNA extraction kit (Solarbio, Beijing, China). The genomic DNA‐binding assay was performed according to a previous method with modification 29 . Bacterial genomic DNA (200 ng) was incubated with increasing amounts of the peptide (100, 200, 400, 600, 800, and 1000 ng) at 28 °C for 90 min.…”

Section: Methodsmentioning

confidence: 99%

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Antibacterial mechanism of the novel antimicrobial peptide Jelleine‐Ic and its efficacy in controlling Pseudomonas syringae pv. actinidiae in kiwifruit

Zhang

Wang

Tang

et al. 2023

Pest Management Science

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BACKGROUNDBacterial canker caused by Pseudomonas syringae pv. actinidiae (Psa) poses a severe threat to kiwifruit production. Because of the insufficient efficacy and environmental safety of available treatments, novel antibacterial agents should be urgently developed. Antimicrobial peptides (AMPs) can be used as antimicrobials for disease control. In this study, we designed a novel AMP, Jelleine‐Ic, and evaluated its antibacterial activity and mechanism of action against Psa.RESULTSJelleine‐Ic with a half‐maximal effective concentration of 1.67 μg/mL exhibited stronger antibacterial activity than did parent Jelleine‐I. Jelleine‐Ic targeted the Psa membrane, increased membrane permeabilization and dissipated membrane potential, resulting in calcium leakage. Electron microscopy revealed that Jelleine‐Ic disrupted cell morphology and caused intracellular alterations. Moreover, this AMP penetrated the cell membrane, bound to DNA, and reduced the expression of genes related to DNA replication and repair. Jelleine‐Ic also reduced esterase activity and induced intracellular reactive oxygen species generation. This peptide inhibited the development of Psa canker. The control efficiency of Jelleine‐Ic against Psa in the leaf discs and leaves of kiwifruit was 81.83% and 70.53%, respectively, which was superior to that of the commercial agricultural streptomycin. Furthermore, Jelleine‐Ic upregulated the expression of kiwifruit defense genes (PR‐10 and WRKY70a).CONCLUSIONJelleine‐Ic effectively controls Psa in vitro and in vivo, and may be developed as a bactericide for plant disease control. © 2023 Society of Chemical Industry.

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Section: Methodsmentioning

confidence: 99%

“…The genomic DNA-binding assay was performed according to a previous method with modification. 29 Bacterial genomic DNA (200 ng) was incubated with increasing amounts of the peptide (100, 200, 400, 600, 800, and 1000 ng) at 28 °C for 90 min. Sterile water served as the negative control.…”

Section: Genomic Dna Bindingmentioning

confidence: 99%

Antibacterial mechanism of the novel antimicrobial peptide Jelleine‐Ic and its efficacy in controlling Pseudomonas syringae pv. actinidiae in kiwifruit

Zhang

Wang

Tang

et al. 2023

Pest Management Science

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“…In vivo, the administration of Jelleine-I to Galleria mellonella infected with the pathogen has shown interesting immunomodulatory effects, including the increase of hemocyte counts, upregulation of other host AMPs, and inhibition of pro-inflammatory cytokines. As a result of the bacterial inhibition, the survival rate of the infected insects increased, and cell proliferation without hemolysis or cytotoxicity was also observed [ 7 ].…”

Section: The Antimicrobial Activity Of Food-sourced Peptidesmentioning

confidence: 99%

“…The bioprotection of AMPs against pathogens benefits the proliferation of probiotic and commensal bacteria in the gut, which also prevents the colonization of the intestine by pathogenic bacteria. Different mechanisms may be involved in the latter, including competitive exclusion, the consumption of available nutrients, the upregulation of host defense genes, and the production of antimicrobial compounds [ 7 , 107 , 108 ].…”

Section: The Antimicrobial Activity Of Food-sourced Peptidesmentioning

confidence: 99%

“…It is noteworthy that recent studies have demonstrated that the cellular receptors for ACE2 are involved in infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the pathogen responsible for the global pandemic of COVID-19 (coronavirus disease 2019) [ 5 , 6 ]. Similar immunomodulatory effects may prevent the progression of foodborne diseases, as observed in the peptide Jelleine-I, from honeybee royal jelly, against Listeria monocytogenes infection (Lepidoptera as a model system) [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

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Bioactive Antimicrobial Peptides from Food Proteins: Perspectives and Challenges for Controlling Foodborne Pathogens

et al. 2023

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Bioactive peptides (BAPs) derived from food proteins have been extensively studied for their health benefits, majorly exploring their potential use as nutraceuticals and functional food components. These peptides possess a range of beneficial properties, including antihypertensive, antioxidant, immunomodulatory, and antibacterial activities, and are naturally present within dietary protein sequences. To release food-grade antimicrobial peptides (AMPs), enzymatic protein hydrolysis or microbial fermentation, such as with lactic acid bacteria (LAB), can be employed. The activity of AMPs is influenced by various structural characteristics, including the amino acid composition, three-dimensional conformation, liquid charge, putative domains, and resulting hydrophobicity. This review discusses the synthesis of BAPs and AMPs, their potential for controlling foodborne pathogens, their mechanisms of action, and the challenges and prospects faced by the food industry. BAPs can regulate gut microbiota by promoting the growth of beneficial bacteria or by directly inhibiting pathogenic microorganisms. LAB-promoted hydrolysis of dietary proteins occurs naturally in both the matrix and the gastrointestinal tract. However, several obstacles must be overcome before BAPs can replace antimicrobials in food production. These include the high manufacturing costs of current technologies, limited in vivo and matrix data, and the difficulties associated with standardization and commercial-scale production.

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Investigating antimicrobial peptide RI12 (K3W) as an effective bio-preservative against Listeria monocytogenes: a major foodborne pathogen

Jha,

Singh

2023

Arch Microbiol

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In vitro and in vivo antimicrobial activity of antimicrobial peptide Jelleine-I against foodborne pathogen Listeria monocytogenes

Cited by 15 publications

References 39 publications

Antibacterial mechanism of the novel antimicrobial peptide Jelleine‐Ic and its efficacy in controlling Pseudomonas syringae pv. actinidiae in kiwifruit

Antibacterial mechanism of the novel antimicrobial peptide Jelleine‐Ic and its efficacy in controlling Pseudomonas syringae pv. actinidiae in kiwifruit

Bioactive Antimicrobial Peptides from Food Proteins: Perspectives and Challenges for Controlling Foodborne Pathogens

Investigating antimicrobial peptide RI12 (K3W) as an effective bio-preservative against Listeria monocytogenes: a major foodborne pathogen

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