In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures

Xiong, Wenbi; Luo, Gang; Zhou, Liming; Zeng, Yun; Yang, Wenji

doi:10.1186/1749-8546-4-14

Cited by 51 publications

(34 citation statements)

References 14 publications

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“…Also ACS demonstrated antitumour effects in malignant cell lines (Xiong et al 2009). ACS inhibited cell growth of human lung adenocarcinoma epithelial cells, human breast adenocarcinoma cell line and human hepatocellular carcinoma cell line in a dose dependent manner.…”

Section: Resultsmentioning

confidence: 99%

Enhanced production of antitumour naphthoquinones in transgenic hairy root lines of Lithospermum canescens

Sykłowska-Baranek

Pietrosiuk

Gawron

et al. 2011

Plant Cell Tiss Organ Cult

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The effects of medium exchange and methyl jasmonate addition on growth and production of shikonin and its derivatives acetylshikonin and isobutyrylshikonin in hairy root cultures of Lithospermum canescens were investigated. Responses varied depending on the transgenic line and stage of growth at which these lines were subjected to treatment. Shikonin itself was not detected, irrespective of the transgenic line and culture treatment used. A eightfold increase in acetylshikonin and isobutyrylshikonin accumulation was achieved when 32-day-old transgenic roots of Lc1D line were transferred from LS to M9 medium for the subsequent 3 weeks of culture. Methyl jasmonate exerted a detrimental effect on red naphthoquinones production. The extracts obtained from roots cultivated in M9 medium for 3 weeks were subjected to a cytotoxicity assay and displayed cytotoxic activity against human promyelocytic leukemia cells (HL-60) at the dose of 4 lg ml -1 .

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Section: Resultsmentioning

confidence: 99%

Enhanced production of antitumour naphthoquinones in transgenic hairy root lines of Lithospermum canescens

Sykłowska-Baranek

Pietrosiuk

Gawron

et al. 2011

Plant Cell Tiss Organ Cult

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“…It was also found that even acetylshikonin inhibited their activities on Siha cells in a time and dose-dependent manner. Based on system biology thinking, it can be considered that the antitumor efficiency of total naphthoquinones results from multi constituents with multiple mechanisms of action, for complex state of cancer, TCM derived multi combinatorial intervention is new desirable strategy (Alley et al, 1988;Van der Greef et al, 2007;Xiong, Luo, Zhou, Zeng & Yang, 2009), specially for overcoming drug resistance of chemotherapy.…”

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confidence: 99%

In vitro anticancer activity of acetylshikonin action against cervical cancer

Sun¹,

Xu²,

Zhang³

et al. 2014

Plant Sci Today

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Cervical cancer is a malignant neoplasm and is the second commonest cancer in women worldwide. Despite the availability of various treatment modalities, the 5-year survival remains poor. Therefore, it is absolutely necessary to explore drugs capable of preventing and treating cervical cancer. Plant-derived natural products are widely used as adjunct to chemotherapy for this type of cancer. Our study aims to investigate the effects of acetylshikonin, an acetyl derivative, isolated from a Chinese medicinal herb Lithospermum erythrorhizon Sieb. et Zucc., a folk medicine with a long history of safe use for clinically treating cervical cancer in China. MTT assay was used to determine the in vitro anticancer effects of acetylshikonin on human cervical cancer Siha cells. The expression of caspase-3 and -8 proteins were determined. Flow cytometry and quantitative RT-PCR were performed to detect the expression level of Siha cells associated markers and regulatory genes. In Siha cell lines, acetylshikonin inhibited cell growth in a dose-dependent manner. The preferential expression of Siha cells related marker, regulatory genes were also highlighted. It arrested the cell cycle by blocking transition from S phase to G2/M phase and induced apoptosis of Siha cell through activating caspase-3 and caspase-8. In the current study, acetylshikonin can be taken as a promising natural lead for cervical cancer.

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“…These derivatives also, exhibit insulin-like activities by inhibiting phosphatase and tensin homologue deleted on Chromosome 10 (PTEN) and protein tyrosine phosphatases (Nigorikawa et al 2006). Studies have also revealed specific in vivo and in vitro antitumor effects of acetylshikonin (Xiong et al 2009). In cell suspension cultures of Lithospermum erythrorhizon, shikonin derivative production is generally inhibited by the addition of NH 4 + (Fujita et al 1981).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical and Morphological Evidences for Shikonin Production by Plant Cell Cultures of Onosma sericeum Willd

Gharehmatrossian

Popov

Ghorbanli

et al. 2016

Braz. arch. biol. technol.

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In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures

Abstract: Background: Shikonin derivatives have cytotoxic and antitumor effects. This study aims to investigate the antitumor effects of acetylshikonin isolated from a Chinese medicinal herb Arnebia euchroma (Royle) Johnst.

Cited by 51 publications

References 14 publications

Enhanced production of antitumour naphthoquinones in transgenic hairy root lines of Lithospermum canescens

Enhanced production of antitumour naphthoquinones in transgenic hairy root lines of Lithospermum canescens

In vitro anticancer activity of acetylshikonin action against cervical cancer

Phytochemical and Morphological Evidences for Shikonin Production by Plant Cell Cultures of Onosma sericeum Willd

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