In vitro and in vivo viability of vitrified and non-vitrified embryos derived from eCG and FSH treatment in rabbit does

Mehaisen, Gamal M. K.; Viudes-de-Castro, M.P.; Vicente, J.S.; Lavara, R.

doi:10.1016/j.theriogenology.2005.08.007

Cited by 36 publications

(42 citation statements)

References 41 publications

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“…Similar EES were found between ULO and intact females, suggesting unilateral ovariectomy did not produce an important hormonal imbalance leading to increased embryo losses, as occurs when hormone-induced superovulation is used (Tsiligianni et al, 2004;Mehaisen et al, 2006). Moreover, unilateral ovariectomy does not seem to change the early embryonic stage of development either.…”

Section: Discussionsupporting

confidence: 62%

The effect of unilateral ovariectomy on early embryonic survival and embryo development in rabbits

et al. 2014

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Unilateral ovariectomy can be used to study uterine capacity in rabbits because an overcrowding of the functional uterine horn is produced. Due to the uterus duplex, the rabbit is the ideal model for such studies. However, this technique may affect embryo survival. The aim of this work is to study the effect of unilateral ovariectomy on early embryo survival and development in rabbit. A total of 101 unilateral ovariectomised females and 52 intact females were compared after slaughter at 30 h post-mating. Early embryo survival was estimated as the ratio between number of embryo recovered and ovulation rate. No differences were found between intact and unilaterally ovariectomised females in this trait. Unilateral ovariectomy did not change embryo development, measured as the number of embryo cells. Variability of embryo development was not affected either. At 30 h post-mating, the majority of embryos (86.2%) were 4-cell stage. Embryo quality was evaluated according to morphological criteria. No difference in embryo quality between intact and unilaterally ovariectomised females was found. Therefore, unilateral ovariectomy performed before puberty in rabbit does not modify early embryo survival and development.

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Section: Discussionsupporting

confidence: 62%

The effect of unilateral ovariectomy on early embryonic survival and embryo development in rabbits

et al. 2014

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“…Among the factors responsible for this situation a visible tendency of less frequent development of in vitro cultured, traditionally vitrified embryos to more advanced blastocyst stages (83.1% vs. 90.6% in control, Table 2) may be indicated. Nevertheless, the ratio of pups born after vitrification shown here (36.8%) remains within the range of data reported recently for rabbit cryopreserved embryo transfer (Vicente and Garcia Ximenez, 1996;Kaufmann et al, 1998;Lopez-Bejar and Lopez-Gatius, 2002;Mehaisen et al, 2006). Further experiments will be needed to compare the transfer efficiency of embryos vitrified using methods presented in this paper (especially those more promising, employing the VitMaster system).…”

supporting

confidence: 84%

“…In spite of this improvement, a relatively low proportion [approximately 65% (Kasai et al, 1992)] of rabbit embryos was able to develop after cryopreservation into viable fetuses in vivo. Apart from issues related to cryopreservation, technical problems with the surgical transfer and the usual use of embryos obtained from superovulated donors may be indicated among the factors responsible for this situation (Kaufmann et al, 1998;Mehaisen et al, 2006). An optimum content of vitrification solution(s) fulfilling embryo demand for cryoprotective efficacy and low toxicity has been of interest to researchers for years.…”

mentioning

confidence: 99%

Comparison of traditional and modified (VitMaster) methods of rabbit embryo vitrification

Papis

Korwin-Kossakowski

Wenta-Muchalska

2009

Acta Veterinaria Hungarica

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In spite of their cryobiological efficacy, minimum-volume vitrification methods suffer from the risk of microbiological contamination and are technically and/or manually demanding. In this study, the effects of a traditional, slightly modified vitrification method and vitrification using supercooled liquid nitrogen (VitMaster) applied for rabbit morula-stage embryos were compared. Embryos were equilibrated in a solution containing 1,2-propanediol (2.72 M) and glycerol (1.36 M) for 7 min and vitrified in 0.25-ml insemination straws after 1-min exposure to a vitrification solution containing additionally 1.0 M sucrose. Cooling was performed in 'normal' or supercooled liquid nitrogen. Regardless of the cooling method applied, high in vitro survival and development rates of vitrified embryos were obtained. All embryos were intact after warming, and 61 out of 65 (93.8%) and 23 out of 24 (95.8%) embryos developed to the blastocyst stage after 48-h in vitro culture of embryos vitrified in 'normal' or supercooled liquid nitrogen, respectively. The results suggest higher developmental ability of embryos vitrified in supercooled liquid nitrogen (91.7% vs. 83.1% of embryos vitrified traditionally developed to more advanced, expanding and/or hatching blastocyst stages). In vivo survival rate, tested for the traditional vitrification system only, revealed that 36.8% of embryos developed to term. The results show promise for establishing a fully successful method for rabbit embryo vitrification.

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“…Embryos were frozen in the same cryoprotective solution containing 1.5 M DMSO and by the same slow freezing process, even more vitrification could provide good results but this method is not totally under control (Mehaisen et al, 2006).…”

Section: Embryos Recovering and Freezingmentioning

confidence: 99%

Cryopreservation of Genetic Diversity in Rabbit Species (Oryctolagus cuniculus)

Joly¹,

Neto²,

Salvetti³

2012

Current Frontiers in Cryopreservation

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In vitro and in vivo viability of vitrified and non-vitrified embryos derived from eCG and FSH treatment in rabbit does

Cited by 36 publications

References 41 publications

The effect of unilateral ovariectomy on early embryonic survival and embryo development in rabbits

The effect of unilateral ovariectomy on early embryonic survival and embryo development in rabbits

Comparison of traditional and modified (VitMaster) methods of rabbit embryo vitrification

Cryopreservation of Genetic Diversity in Rabbit Species (Oryctolagus cuniculus)

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