In vitro and in vivo quantification of elicitin expression in Phytophthora cinnamomi

Horta, M.; Sousa, Nelson; Coelho, A. C.; Neves, Dina; Cravador, A.

doi:10.1016/j.pmpp.2009.02.003

Cited by 28 publications

(36 citation statements)

References 48 publications

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“…The choice of actin mRNA as a stable endogenous control to normalize the amount of sample RNA was validated by evaluation of the oomycete actin mRNA levels in in vitro and in planta conditions. In RNA extracted from various in vitro cultures both bands (18S and 28S), had identical intensity, showing that an identical quantity of Phytophthora RNA was present, the intensity of actin mRNA bands was similar in all samples, showing that actin mRNA levels were also identical [23].…”

Section: Quantification Of Gip Transcriptsmentioning

confidence: 83%

Cloning, characterization and in vitro and in planta expression of a glucanase inhibitor protein (GIP) of Phytophthora cinnamomi

Martins

Belo

et al. 2014

Mol Biol Rep

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Oomycetes from the genus Phytophthora are fungus-like plant pathogens that are devastating for agriculture and natural ecosystems. They are able to secrete a glucanase inhibitor protein (GIP) that inhibits the activity of endoglucanases (EGases) involved in defense responses against infection. One of the most widely distributed and aggressive Phytophthora species, with more than 1,000 host plants is P. cinnamomi. In this work we report the sequencing and characterization of a class of GIPs secreted by Phytophthora cinnamomi. The gip gene from P. cinnamomi has a 937 bp ORF encoding a putative peptide of 312 deduced amino acids. The expression of this gene was studied during growth in different carbon sources (glucose, cellulose and sawdust), by RT-qPCR and its level of expression was evaluated at five time points. The highest expression of gip gene occurred in sawdust at 8 h of induction. In vivo infection of C. sativa revealed an increase in gip expression from 12 to 24 h. At 36 h its expression decreased suggesting that a compensatory mechanism must occur in plant.

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Section: Quantification Of Gip Transcriptsmentioning

confidence: 83%

Cloning, characterization and in vitro and in planta expression of a glucanase inhibitor protein (GIP) of Phytophthora cinnamomi

Martins

Belo

et al. 2014

Mol Biol Rep

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“…The fungus Gibberella fujikuroi was kindly provided by Dr. B. Fraga, Natural Products and Agrobiology Institute, CSIC, Canary Islands, Spain. The agar dilution technique was used to evaluate the effect of the prenylated compounds on the growth of P. cinnamomi 41,42 , G. fujikuroi 43 and B. cinerea 44,45 . Fungicidal activity assays of these compounds were performed in microcultures by growing the fungi in sterile Petri dishes (35 mm) at a final volume of 2 mL medium containing different compound concentrations.…”

Section: In Vitro Fungicidal Activity Assaysmentioning

confidence: 99%

Synthesis, Anti-Phytopathogenic and DPPH Radical Scavenging Activities of C-Prenylated Acetophenones and Benzaldehydes

Osorio

Quiroz

Carvajal

et al. 2016

J. Chil. Chem. Soc.

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The syntheses of six prenylated acetophenone and benzaldehyde derivatives and their anti-phytopathogenic and antioxidant activities are reported. These compounds were obtained by electrophilic aromatic substitution (S E Ar) of the corresponding arenes and 3-methyl-2-buten-1-ol using ZnCl 2 as a Lewis acid catalyst in ethyl acetate. Reasonable to good yields were obtained based on unrecovered aromatic starting material (45-73%). All the synthesized compounds were evaluated against phytopathogenic gram-negative bacteria Agrobacterium tumefaciens, Pseudomonas syringae and Erwinia carotovora and plant fungal pathogens Botrytis cinerea, Phytophthora cinnamomi and Gibberella fujikuroi. The antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay and expressed as half maximal inhibitory concentration (IC 50 ) values in µM concentrations. The antioxidant activity went from 27.20 µM to >100 µM. Compound 11 showed statistically significant inhibition of the growth of Botrytis cinerea, and compounds 13 and 15 showed statistically significant inhibition of the growth of Phytophthora cinnamomi, with respect to negative control fungal growth. All six compounds showed bacteriostatic effects against gram-negative plant pathogenic bacteria with IC 50 values between 250 and <3.9 µM.

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“…In P. cinnamomi, a gene cluster consisting of four elicitin genes has been identified by Duclos et al (1998). It was recently shown that the β-cinnamomin gene (β-cin) is transcribed during the active growth of the pathogen when infecting newly germinated cork oak roots, and that the effect of silencing the β-cin also reduces the expression level of other elicitin genes in the cluster (Horta et al 2008); furthermore, at the phenotypic level, it delays the in planta growth of the pathogen.…”

Section: Introductionmentioning

confidence: 99%

Involvement of the β-cinnamomin elicitin in infection and colonisation of cork oak roots by Phytophthora cinnamomi

et al. 2010

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The virulence of two wild type (PA45 and PA37) and two genetically modified (13C: hygromycin resistant; FATSS: hygromycin resistant and β-cin knock-down) Phytophthora cinnamomi strains towards cork oak (Quercus suber) was assessed via a quantitative evaluation of disease symptoms arising from a soil infestation assay, and by a histological analysis of root colonization. Comparison of virulence, as expressed by symptom severity, resulted in the following ranking: highly virulent (wild type strains), medium virulence (strain 13C) and weakly virulent (FATSS). Both transgenic strains were compromised in their virulence, as expressed by symptom severity, but strain 13C was much less affected than FATSS. Microscopic observation showed that the FATSS strain was unable to effectively invade the root, while 13C and the two wild type strains were all able to rapidly colonize the whole root, including the vascular tissue. These results strengthen the notion that elicitins are associated, either directly or indirectly, with the infection process of Phytophthora.

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In vitro and in vivo quantification of elicitin expression in Phytophthora cinnamomi

Cited by 28 publications

References 48 publications

Cloning, characterization and in vitro and in planta expression of a glucanase inhibitor protein (GIP) of Phytophthora cinnamomi

Cloning, characterization and in vitro and in planta expression of a glucanase inhibitor protein (GIP) of Phytophthora cinnamomi

Synthesis, Anti-Phytopathogenic and DPPH Radical Scavenging Activities of C-Prenylated Acetophenones and Benzaldehydes

Involvement of the β-cinnamomin elicitin in infection and colonisation of cork oak roots by Phytophthora cinnamomi

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