In vitro and in vivo immunoregulatory activity of sulfated fucan from the sea cucumber A. leucoprocta

Guo, Feng; Su, Laijin; Shen, Chen; Wang, Teng; Zhou, Da‐Yang; Chen, Yin; Guo, Shoudong

doi:10.1016/j.ijbiomac.2021.08.008

Cited by 16 publications

(9 citation statements)

References 31 publications

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“…IL-1β, the major endogenous pyrogen, possessed multiple properties in response to infection, injury, and immune alteration [ 45 ]. The production of cytokines, specifically TNF-α, IL-6, and IL-1β in macrophage cell line RAW264.7, was enhanced by water-soluble sulfated fucan from the sea cucumber A. leucoprocta [ 46 ]. Likewise, the expressions of TNF-α and IL-6 were upregulated by Holothuria leucospilota polysaccharides in CTX-induced immunosuppressed mice [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Holothurian Wall Hydrolysate Ameliorates Cyclophosphamide-Induced Immunocompromised Mice via Regulating Immune Response and Improving Gut Microbiota

Chen

et al. 2023

IJMS

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Some biologically active compounds isolated from sea cucumbers stimulate the body’s immune response by activating immune cells. Immune function is closely related to the integrity intestinal barrier and balanced gut microbiota. However, it is unknown whether the daily administration of holothurian wall hydrolysate (HWH) ameliorated intestinal dysbiosis and barrier injury induced by immunodeficiency. This study aimed to investigate the immunomodulatory effect and the underlying mechanism of HWH in cyclophosphamide (CTX)-induced immunocompromised mice. BALB/c mice received CTX (80 mg/kg, intraperitoneally) once a day for 3 days to induce immunodeficiency, and then they received the oral administration of HWH (80 or 240 mg/kg) or levamisole hydrochloride (LH, 40 mg/kg, positive control), respectively, once a day for 7 days. We utilized 16S rRNA sequencing for microbial composition alterations, histopathological analysis for splenic and colonic morphology, Western blotting for expressions of tight junction proteins (TJs), and quantitative real-time (qRT)-PCR for measurements of pro-inflammatory cytokines. HWH attenuated the immune organ damage induced by CTX, increased the secretions of interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF)-α, and promoted the recovery of goblet cells and the production of TJs (claudin-1, occludin, and ZO-1) in the colon of the immunocompromised mice. Moreover, HWH promoted the growth of beneficial microorganisms such as Lactobacillus, Lachnospiraceae, Christensenellaceae, and Bifidobacterium, while it suppressed the populations of Ruminococcus, Staphylococcus, and Streptococcus. These results demonstrate that HWH elicits intestinal mucosal immunity, repairs the damage to intestinal mucosal integrity, and normalizes the imbalanced intestinal microbial profiles in immunocompromised mice. It may be helpful to identify the biological activities of HWH to support its potential use in new prebiotics, immunomodulatory agents, and medical additives for intestinal repair.

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Section: Discussionmentioning

confidence: 99%

Holothurian Wall Hydrolysate Ameliorates Cyclophosphamide-Induced Immunocompromised Mice via Regulating Immune Response and Improving Gut Microbiota

Chen

et al. 2023

IJMS

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“…In addition, fucoidan can also activate Nrf2/HO-1 pathway, by increasing Nrf2, GCLM, HO-1 and NQO1 protein levels. Sulfate polysaccharide AL1-1 in sea cucumber can significantly increase intestinal total antioxidant capacity (T-AOC) and decrease intestinal MDA level ( 42 ). AL1-1 can also increase the levels of CAT and GSH-Px, and significantly enhance the immune response.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Oyster Peptides Derived From Crassostrea gigas on Intestinal Oxidative Damage Induced by Cyclophosphamide in Mice Mediated Through Nrf2-Keap1 Signaling Pathway

Chen

Zheng

et al. 2022

Front. Nutr.

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Oyster peptide (OP) has exhibited useful biological activities and can be used in multi-functional foods. OP has been reported to play a significant role in intestinal protection, but its specific mechanism is still not completely understood. The aim of this study was to analyze the potential effect of OP on oxidative damage of mice intestine induced by cyclophosphamide (Cy). The experimental results revealed that intragastric administration of OP significantly increased average bodyweight, improved ileum tissue morphology and villus structure, as well as increased the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in oxidized mice serum and liver. The content of malondialdehyde (MDA) in the mice serum and liver homogenate was found to be markedly decreased. Moreover, OP significantly increased the relative mRNA expression levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), quinone oxidoreductase (NQO1) and heme oxidase-1 (HO-1) in ileum. Western-blot results indicated that prior administration of OP significantly up-regulated the Nrf2 production in ileum, and substantially decreased then Keap1 gene expression. In conclusion, intake of OP was found to markedly improve intestinal oxidative stress in vivo, and this effect was primarily mediated through the simulation of antioxidant Nrf2-Keap1 signaling pathway. This study is beneficial to the application of peptide nutrients in the prevention or mitigation of intestinal oxidative damage.

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“…Intragastrical administered Sulfated fucan at concentrations of 50 mg/kg, 100 mg/kg, 200 mg/kg for 7 days promoted intestinal immunity by increasing secretory immunoglobulins A. It also exhibited antioxidant activity by increasing the antioxidant enzyme levels of T-AOC, SOD, GSH-Px and CAT and decreasing the enzyme amount of MDA on the cyclophosphamide triggered immunosuppression mouse model (Feng et al, 2021). Wang et al (2017) studied the immunomodulatory activities of Apostichopus japonicas-derived glycosaminoglycan in cyclophosphamide-induced immunosuppressed mice and reported a significant increase in splenic lymphocyte proliferation, splenocytes Ca2+ concentration, total antioxidant capacity, superoxidase dismutase, catalase and glutathione peroxidase activities while reducing malondialdehyde levels in mouse heart, kidney, and liver.…”

Section: Sulfated Fucanmentioning

confidence: 98%

In Vivo and Clinical Studies of Sea Cucumber-Derived Bioactives for Human Health and Nutrition From 2012-2021

et al. 2022

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Sea cucumbers are amongst the highest value seafoods available commercially, especially in the south-east Asian region, primarily due to their nutritional and health benefits as applied in Traditional Chinese Medicine. While the majority of studies for nutritional products derived from sea cucumber compounds have been conducted in vitro, the number of in vivo and evidence-based human clinical studies are limited. This review has critically assessed the advances in in vivo and clinical studies of sea cucumber-derived bioactives (both extracts and compounds) via a comprehensive literature research on papers published in the last ten years from 2012-2021. Sea cucumber-derived compounds were reported to have the following activities: anticancer, antihyperlipidemic, antihyperglycemic, anti-inflammatory, anticoagulant/antithrombotic, antioxidant, and antihypertension, immunomodulatory, wound healing and as treatments for Alzheimer’s and Parkinson’s diseases. These active compounds include triterpene glycosides (saponins), fucosylated chondroitin sulfate (FCS), cerebrosides, glycosaminoglycan, fucoidan, phospholipids, polysaccharides, peptides, long-chain bases, Frondanol A5, acid mucopolysaccharide, and phosphatidylcholines. Gaps, challenges and future directions have been identified and discussed separately to progress different areas of research and to further scientific validation, development and application of sea cucumbers for human health and nutritional products.

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In vitro and in vivo immunoregulatory activity of sulfated fucan from the sea cucumber A. leucoprocta

Cited by 16 publications

References 31 publications

Holothurian Wall Hydrolysate Ameliorates Cyclophosphamide-Induced Immunocompromised Mice via Regulating Immune Response and Improving Gut Microbiota

Holothurian Wall Hydrolysate Ameliorates Cyclophosphamide-Induced Immunocompromised Mice via Regulating Immune Response and Improving Gut Microbiota

Protective Effect of Oyster Peptides Derived From Crassostrea gigas on Intestinal Oxidative Damage Induced by Cyclophosphamide in Mice Mediated Through Nrf2-Keap1 Signaling Pathway

In Vivo and Clinical Studies of Sea Cucumber-Derived Bioactives for Human Health and Nutrition From 2012-2021

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