In vitro and in vivo Antidiabetic Properties of Phenolic Antioxidants From Sedum adenotrichum

Abstract

Natural products serve as the mainstay of human life, and today, almost half of the drugs in clinical practice are the natural origin. Keeping in view the importance of medicinal plants and natural products, Sedum adenotrichum also known as Rosularia adenotrichum was selected for the present study. The crude extract of S. adenotrichum whole plant was obtained through a rotary evaporator. The extract was analyzed for a polyphenolic profile using high-performance liquid chromatography with a diode-array detector. The extract was subjected to detail in vivo antidiabetic study. In this study, body weight, blood glucose level, glycated hemoglobin, lipid profile, liver function tests, and renal function tests were performed in animal models. The extract was tested for in vitro α-glucosidase inhibition activity. Results of high-performance liquid chromatography with a diode-array detector chromatogram revealed a total of 22 polyphenolic compounds. No major change in body weight was noted in experimental animals. Alloxan induction led to a significant elevation in plasma glucose level. A significant decline was noted in blood glucose and glycated hemoglobin concentration in rats treated with the extract as well as with glibenclamide. Renal/liver function tests, lipid profile, alkaline phosphatase, and serum cholesterol were normalized by the extract-treated rats. The α-glucosidase inhibitory activity at 62.5 and 1,000 μg/ml was noted to be 63.97 and 80.80, respectively, both approaching to standard. The results reveal that the extract was rich in important phenolic compounds. In the antidiabetic potentials of the crude extract, there might be involved several pancreatic and extra-pancreatic mechanisms acting synergistically to induce the potent antidiabetic effect.

Evaluation of Antioxidant, Analgesic and Anti-Inflammatory Properties of Sedum Adenotrichum

“…The methanolic extract of Sedum contains phenolic compounds at the lower concentration of below 100 µg/g of quinic acid, p-hydroxybenzoic acid, gallic acid, salicylic acid, catechin, 3-Ocoumaroylquinic acid, and naringenin. Kaempferol oyl-glucoside)-7-glucoside, kaempferol-3,7-diglucoside, kaempferol-3-(p-coumaroyl-diglucoside)-7glucoside and 4-caffeoyl-5-coumaroylquinic acid were major compounds as reported in our recent work (10). Three phenolic compounds namely kaempferol-3-O-sophoroside (3.36 mg/g), vitexin-6-malonyl-2-xyloside (2.1 mg/g), quercetin-3,4diglucoside-3-(6-feruloyl glucoside) (1.8 mg/g) and diosmetin-7-rutinoside (1.6 mg/g) were present in significantly higher amounts.…”

“…The solvent was completely evaporated using an evaporator (Heidolph, Germany) and the gummy extract was poured into separate bottles and labeled. The phenolic composition of the extract was studied using HPLC-DAD and the results were recently reported (10).…”

“…However, there is a lack of literature regarding its phytochemical composition and medicinal properties. In our recent study (10), we have shown the phenolic profile of the plant. The present study was aimed to explore the scientific basis of the traditional use of the species for possible in vitro antioxidant and in vivo antinociceptive and antiinflammatory potentials.…”

“…Several species in this genus possess high economic values as medicines, food, and ornamentals. Recently, Sedum adenotrichum has in vitro and in vivo anti-diabetic properties (10). Some are toxic or allergenic which can adversely affect humans and animals (11).…”

Concept, mechanism, and applications of phenolic antioxidants in foods

“…Similarly, strong inhibitory activity had been shown by myricetin against α-amylase, α-glucosidase, and lipase found in black legumes such as black soybean and black turtle beans (Tan et al, 2017). Recently, phenolic antioxidants from Sedum adenotrichum leave extract showed to possess antidiabetic properties in both in vitro and in vivo model (Naz, Muhamad, Zeb, & Shah, 2019). Lipid peroxidation in diabetic rats was alleviated by ferulic acid supplementation (Balasubashini, Rukkumani, Viswanathan, & Menon, 2004).…”