In vitro and in vivo germination of papaya (Carica papaya L.) seeds

Bhattacharya, Jayanta; Khuspe, S. S.

doi:10.1016/s0304-4238(01)00237-0

Cited by 58 publications

(37 citation statements)

References 14 publications

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“…1, 3). These results are consistent with those of Nikolic et al (2006) and Bhattacharya and Khuspe (2001), who reported that most of the cytokinins including BA and TDZ influenced seed germination in Lotus corniculatus and Carica papaya. However, seeds were also placed on control MS0 (MSmedium without PGRs) which showed germination after 38-45 days.…”

Section: Resultssupporting

confidence: 92%

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Short Communication. Factors influencing in vitro seeds germination and correlation of antioxidant activity with tissue development in Cedrus deodara

et al. 2013

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Aim of study: The main objective of this study is to introduce a reliable system for in vitro seed germination. Because Cedrus deodara stands are gradually decreasing in different regions of the world due to overexploitation, natural disasters and lower seed viability.Area of study: Swat is situated at the northwestern corner of Pakistan; with total area of 5337 square km. Total cultivated land is 95281 hectares in 2007, while the area under forest cover is 135427 hectares.Material and Methods: We used MS-medium with or without PGRs for overall in vitro seed germination. To enhance germination frequency, we applied different photoperiods (16hrs-Dark/8hrs-Light and 16hrs-Light/8hrs-Dark) and sterilization reagents (mercuric chloride and ethanol). Synthetic free radical of DPPH (1, 1-diphenyl-2-picrylhydrazyl) was applied for the determination of antioxidant activityMain results: Maximum shoots length (3.6 cm) and root length (2.6 cm) were recorded on MS-medium augmented with BA (1.0 mg L-1) and (GA3) 0.5 mg L-1) under light incubation (16hrs-Light/8hrs-Dark) after 2-3 weeks of inoculation. Without PGRs, maximum root length (4.0 cm, with shoots of 3.2 cm) was observed in dark incubation (16hrs-Dark/8hrs-Light), whereas light incubation produced maximum shoot length (3.5 cm) and minimum root length (1.5 cm). Lower concentration of HgCl2 (0.1%) showed a lower inhibitory effect on shoot and root length (2.4 cm and 2.5 cm) as compared to higher concentrations. The antioxidant potential was also investigated in different Cedrus organs and tissues.Research highlights: These results suggested that this simple protocol is useful for Cedrus deodara conservation and plantlets production for commercial purposes.Key Words: Cedrus deodara; Seed germination; Callus; 6-Benzyladenine; Antioxidant.

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Section: Resultssupporting

confidence: 92%

“…2-e). Similarly, Bhattacharya and Khuspe (2001) reported that in vitro conditions along with light-dark incubation is very effective for seed germination in different cultivars of papaya.…”

Section: Resultsmentioning

confidence: 91%

Short Communication. Factors influencing in vitro seeds germination and correlation of antioxidant activity with tissue development in Cedrus deodara

et al. 2013

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“…The availability of completed 372 Mbp papaya genome with various bioinformatics tools and resources accelerates the identification of genes involved in important biological processes (Ming et al 2008). Molecular studies in papaya have been facilitated by the establishment of in vitro culture and transformation system, including numerous studies in callus induction and regeneration from various explants (Ascencio-Cabral et al 2008;Bhattacharya and Khuspe 2001;Chen and Chen 1992;Chen et al 1987;Fitch et al 1993;Conover 1981, 1982;Sun et al 2011;Yu et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide transcriptome profiling of Carica papaya L. embryogenic callus

Jamaluddin

Noor

Goh

2017

Physiol Mol Biol Plants

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Genome-wide transcriptome profiling is a powerful tool to study global gene expression patterns in plant development. We report the first transcriptome profile analysis of papaya embryogenic callus to improve our understanding on genes associated with somatic embryogenesis. By using 3 0 mRNA-sequencing, we generated 6,190,687 processed reads and 47.0% were aligned to papaya genome reference, in which 21,170 (75.4%) of 27,082 annotated genes were found to be expressed but only 41% was expressed at functionally high levels. The top 10% of genes with high transcript abundance were significantly enriched in biological processes related to cell proliferation, stress response, and metabolism. Genes functioning in somatic embryogenesis such as SERK and LEA, hormone-related genes, stress-related genes, and genes involved in secondary metabolite biosynthesis pathways were highly expressed. Transcription factors such as NAC, WRKY, MYB, WUSCHEL, Agamous-like MADS-box protein and bHLH important in somatic embryos of other plants species were found to be expressed in papaya embryogenic callus. Abundant expression of enolase and ADH is consistent with proteome study of papaya somatic embryo. Our study highlights that some genes related to secondary metabolite biosynthesis, especially phenylpropanoid biosynthesis, were highly expressed in papaya embryogenic callus, which might have implication for cell factory applications. The discovery of all genes expressed in papaya embryogenic callus provides an important information into early biological processes during the induction of embryogenesis and useful for future research in other plant species.

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“…Most commercial plantations are established from seedderived plants, resulting in crops with low profitability because of heterogeneity and genetic variation displayed by the plants after cross-pollination (Bhattacharya and Khuspe 2001).…”

Section: Introductionmentioning

confidence: 99%

Polyethylene glycol effects on somatic embryogenesis of papaya hybrid UENF/CALIMAN 01 seeds

Heringer¹,

Vale²,

Barroso³

et al. 2013

Theor. Exp. Plant Physiol.

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Among commercial fruits, papaya (Carica papaya L.) is notably one of the most common ones around the world. The aim of the current study was to establish a somatic embryogenesis protocol for the hybrid UENF/CALIMAN 01, elucidating the effects of ). The 60 g L -1 PEG treatment group had the highest average for the number, maturation and conversion of somatic embryos that resulted in plantlets at the end of the process. The addition of PEG to MS medium led to increased protein synthesis. These results are unprecedented for C. papaya hybrid UENF/CALIMAN 01 as the current study demonstrates the development of somatic embryogenesis in this high quality hybrid.

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In vitro and in vivo germination of papaya (Carica papaya L.) seeds

Cited by 58 publications

References 14 publications

Short Communication. Factors influencing in vitro seeds germination and correlation of antioxidant activity with tissue development in Cedrus deodara

Short Communication. Factors influencing in vitro seeds germination and correlation of antioxidant activity with tissue development in Cedrus deodara

Genome-wide transcriptome profiling of Carica papaya L. embryogenic callus

Polyethylene glycol effects on somatic embryogenesis of papaya hybrid UENF/CALIMAN 01 seeds

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