In vitro and in vivo exploration of palmitic acid from Synechococcus elongatus as an antibiofilm agent on the survival of Artemia franciscana against virulent vibrios

Santhakumari, Sivasubramanian; Nilofernisha, Nizam Mohamed; Ponraj, Jeyaraj Godfred; Pandian, Shunmugiah Karutha; Ravi, Arumugam Veera

doi:10.1016/j.jip.2017.09.001

Cited by 50 publications

(19 citation statements)

References 44 publications

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“…Equal amount of dried EPS samples were taken from control and QA-UDA combination treated samples for FTIR analysis ( Santhakumari et al, 2017 ). The samples were mixed with potassium bromide (KBr), 64 scans were taken from 4000 to 400 cm -1 with spectral resolution of 4 cm -1 in a FTIR spectrometer (Nicolet iS5, Thermo Scientific, Marietta, GA, United States).…”

Section: Methodsmentioning

confidence: 99%

Synergistic Effect of Quinic Acid Derived From Syzygium cumini and Undecanoic Acid Against Candida spp. Biofilm and Virulence

et al. 2018

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In recent decades, fungal infections have incredibly increased with Candida genus as the major cause of morbidity and mortality in hospitalized and immunocompromised patients. Most of the Candida species are proficient in biofilm formation on implanted medical devices as well as human tissues. Biofilm related Candida infections are very difficult to treat using common antifungal agents owing to their increased drug resistance. To address these issues, the present study investigated the antibiofilm and antivirulent properties of Syzygium cumini derived quinic acid in combination with known antifungal compound undecanoic acid. Initially, antibiofilm potential of S. cumini leaf extract was assessed and the active principles were identified through gas chromatography and mass spectrometry analysis. Among the compounds identified, quinic acid was one of the major compounds. The interaction between quinic acid and undecanoic acid was found to be synergistic in the Fractional inhibitory concentration index (≤0.5). Results of in vitro assays and gene expression analysis suggested that the synergistic combinations of quinic acid and undecanoic acid significantly inhibited virulence traits of Candida spp. such as the biofilm formation, yeast-to-hyphal transition, extracellular polymeric substances production, filamentation, secreted hydrolases production and ergosterol biosynthesis. In addition, result of in vivo studies using Caenorhabditis elegans demonstrated the non-toxic nature of QA-UDA combination and antivirulence effect against Candida spp. For the first time, synergistic antivirulence ability of quinic acid and undecanoic acid was explored against Candida spp. Thus, results obtained from the present study suggest that combination of phytochemicals might be used an alternate therapeutic strategy for the prevention and treatment of biofilm associated Candida infection.

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Section: Methodsmentioning

confidence: 99%

Synergistic Effect of Quinic Acid Derived From Syzygium cumini and Undecanoic Acid Against Candida spp. Biofilm and Virulence

et al. 2018

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“…(SK-3); phenethylamides and a cyclic dipeptide isolated from Halobacillus salinus, Bacillus or Halobacillus sp. ; palmitic acid from Synechococcus elongatus (Abed et al, 2013;Santhakumari et al, 2016Santhakumari et al, , 2017. Taken together, our knowledge about QS system and potential QQs in extremophiles guided us to hypothesize that extremophilic microorganisms can adapt to harsh conditions by producing some other unstudied metabolites to compete with other organisms.…”

Section: Discussionmentioning

confidence: 91%

Extremophilic Natrinema versiforme Against Pseudomonas aeruginosa Quorum Sensing and Biofilm

et al. 2020

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Pseudomonas aeruginosa is an opportunistic pathogen that causes high morbidity and mortality rates due to its biofilm form. Biofilm formation is regulated via quorum sensing (QS) mechanism and provides up to 1000 times more resistance against conventional antibiotics. QS related genes are expressed according to bacterial population density via signal molecules. QS inhibitors (QSIs) from natural sources are widely studied evaluating various extracts from extreme environments. It is suggested that extremely halophilic Archaea may also produce QSI compounds. For this purpose, we tested QS inhibitory potentials of ethyl acetate extracts from cell free supernatants and cells of Natrinema versiforme against QS and biofilm formation of P. aeruginosa. To observe QS inhibition, all extracts were tested on P. aeruginosa lasB-gfp, rhlA-gfp, and pqsA-gfp biosensor strains and biofilm inhibition was studied using P. aeruginosa PAO1. According to our results, QS inhibition ratios of cell free supernatant extract (CFSE) were higher than cell extract (CE) on las system, whereas CE was more effective on rhl system. In addition, anti-biofilm effect of CFSE was higher than CE. Structural analysis revealed that the most abundant compound in the extracts was trans 4-(2-carboxy-vinyl) benzoic acid.

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“…During the AHPND pathogenesis, V. harveyi colonizes on the shrimp hepatopancreas (HP), in which it causing tissue destruction and dysfunction of the HP and digestive organs 62 . Hence, there is an increasing attention in the development of effective anti-infection agents to control the Vibrio mediated bacterial infections in aquaculture through anti-biofilm or anti-quorum sensing (anti-QS) approach in recent years 23 , 24 .…”

Section: Resultsmentioning

confidence: 99%

“…Biofilms are an aggregated growth pattern of bacterial pathogens, which acts vital role in their pathogenesis to cause infectious diseases to the host 63 , 64 . Hence, hindering biofilm formation by anti-biofilm agents has considered a promising mode to trim-down the infection rate in aquaculture 23 . Recently, Soowannayan et al 65 have successfully protected AHPND infected shrimps ( P. vannamei ) using Vibrio biofilm inhibitors.…”

Section: Resultsmentioning

confidence: 99%

“…This bacterium has well renowned to cause diseases such as acute hepatopancreatic necrosis disease (AHPND), vibriosis, septic hepatopancreatic necrosis (SHPN), skin ulcer, gastroenteritis, vasculitis, Bolitas negricans, eye lesions, and deep dermal lesions 22 . The biofilm formation and virulence factor production by bacterial pathogens attributed to their pathogenesis, which regulated by cell-density dependent gene expression, known as quorum sensing 23 . Therefore, antibiotics have been used to control bacterial infections in aquaculture so far.…”

Section: Introductionmentioning

confidence: 99%

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Metal sensing-carbon dots loaded TiO2-nanocomposite for photocatalytic bacterial deactivation and application in aquaculture

Alexpandi

Gopi

Durgadevi

et al. 2020

Sci Rep

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Nowadays, bioactive nanomaterials have been attracted the researcher’s enthusiasm in various fields. Herein, Diplocyclos palmatus leaf extract-derived green-fluorescence carbon dots (DP-CDs) were prepared using the hydrothermal method. Due to the strong fluorescence stability, the prepared DP-CDs were coated on filter-paper to make a fluorometric sensor-strip for Fe 3+ detection. After, a bandgap-narrowed DP-CDs/TiO 2 nanocomposite (DCTN) was prepared using the methanolic extract of D. palmatus. The prepared DCTN exhibited improved photocatalytic bacterial deactivation under sunlight irradiation. The DCTN-photocatalysis slaughtered V. harveyi cells by the production of reactive oxygen species, which prompting oxidative stress, damaging the cell membrane and cellular constituents. These results suggest the plausible mode of bactericidal action of DCTN-photocatalysis under sunlight. Further, the DCTN has shown potent anti-biofilm activity against V. harveyi , and thereby, DCTN extended the survival of V. harveyi -infected shrimps during the in vivo trial with Litopenaeus vannamei . Notably, this is the first report for the disinfection of V. harveyi -mediated acute-hepatopancreatic necrosis disease (AHPND) using nanocomposite. The reduced internal-colonization of V. harveyi on the hepatopancreas as well as the rescue action of the pathognomonic effect in the experimental animals demonstrated the anti-infection potential of DCTN against V. harveyi -mediated AHPND in aquaculture.

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In vitro and in vivo exploration of palmitic acid from Synechococcus elongatus as an antibiofilm agent on the survival of Artemia franciscana against virulent vibrios

Cited by 50 publications

References 44 publications

Synergistic Effect of Quinic Acid Derived From Syzygium cumini and Undecanoic Acid Against Candida spp. Biofilm and Virulence

Synergistic Effect of Quinic Acid Derived From Syzygium cumini and Undecanoic Acid Against Candida spp. Biofilm and Virulence

Extremophilic Natrinema versiforme Against Pseudomonas aeruginosa Quorum Sensing and Biofilm

Metal sensing-carbon dots loaded TiO2-nanocomposite for photocatalytic bacterial deactivation and application in aquaculture

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