In vitro and in vivo evaluation of antioxidant properties of wild-growing plants. A short review

Fierăscu, Radu Claudiu; Orţan, Alina; Fierascu, Ioana Catalina; Fierăscu, Irina

doi:10.1016/j.cofs.2018.08.006

Cited by 42 publications

(39 citation statements)

References 50 publications

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“…The CPC fractionation of EAF was performed with the biphasic solvent system methyl tert-butyl ether (MtBE)/acetonitrile (CH 3 CN)/H 2 O (3/3/4, v/v/v) to afford 11 subfractions (EAF 1-11 ). After 13 C NMR analyses of EAF [1][2][3][4][5][6][7][8][9][10][11] , all spectra of the fraction series were processed and submitted to hierarchical clustering analysis (HCA) for the recognition of the similarity between emergence profiles of 13 C NMR peaks throughout the fractionation process. In this way, 13 C NMR signals belonging to the same compounds were grouped to build "chemical shift clusters" represented in the heat map drawn in Figure 1.…”

Section: Resultsmentioning

confidence: 99%

“…Fractions of 20 mL were collected over the whole process. All fractions were analyzed by TLC and HPLC and then pooled to yield fractions EAF [1][2][3][4][5][6][7][8][9][10][11] .…”

Section: Centrifugal Partition Chromatographymentioning

confidence: 99%

“…Fraction EAF 2 was subjected to semipreparative HPLC using the gradient system (15%-40% CH 3 CN, 30 minutes) to yield compounds 2 (t R = 5.9 minutes, 3.8 mg), 4 (t R = 9.5 minutes, 4.3 mg), and the mixture of 1 and 3 (t R = 20.2 minutes, 5.1 mg). Fractions EAF [3][4] were purified by semipreparative HPLC with gradient system (10%-40% CH 3 CN, 30 minutes) to yield compounds 1 and 3 in mixture (t R = 12.6 minutes, 2.7 mg), 5 (t R = 13.4 minutes, 3.8 mg), and 6 (t R = 13.8 minutes, 3.7 mg). Fraction EAF 7 was purified by semipreparative HPLC with gradient system (10%-25% CH 3 CN, 20 minutes) to yield compound 7 (t R = 11.2 minutes, 4 mg).…”

Section: Hplc Purification Of Eafmentioning

confidence: 99%

“…2,3 Plant extracts can contain a high concentration of phenolic compounds, carotenoids, terpenoids, etc., able to inhibit free radicals and therefore to slow down skin aging. 4,5 The antioxidant potency of phenolic compounds (phenolic acids, flavonoids, anthocyanidins, tannins) can be easily determined by chemical tests such as 2,2,1-diphenyl-1-picrylhydrazyl (DPPH) assay, the ferric reducing/antioxidant power (FRAP) assay, the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay, and the cupric ion reducing antioxidant capacity (CUPRAC) assay. 4 Phenolics have also been reported as effective compounds in matrix remodeling activation through inhibition of elastases, 6 a class of proteases highly involved in aging.…”

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confidence: 99%

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Investigation of Antioxidant and Elastase Inhibitory Activities ofGeum urbanumAerial Parts, Chemical Characterization of Extracts Guided by Chemical and Biological Assays

Schmitt

Magid

Hubert

et al. 2020

Natural Product Communications

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This study presents the bioguided chemical investigation of the 80% aqueous methanol extract of Geum urbanum aerial parts. Liquid–liquid partitioning of this extract in solvents of increasing polarity combined with biological screening showed that the ethyl acetate (EtOAc) soluble fraction was the most active part of the extract. This fraction was chemically profiled by a 13C nuclear magnetic resonance (NMR)-based dereplication method, resulting in the identification of 14 compounds. The dereplication process was followed by the purification of unknown and minor compounds of the EtOAc fraction. A new glycosylated phenol, namely, 3-(3,4-dihydroxyphenyl)propyl-α-l-rhamnopyranoside, together with 6 known compounds were isolated. Their structures were elucidated by spectroscopic methods including NMR and high-resolution electrospray ionization mass spectrometry. The antioxidant activity of fractions and isolated compounds were evaluated by 2,2,1-diphenyl-1-picrylhydrazyl and hydroxyl radical scavenging, and by cupric ion reducing antioxidant capacity assays. In parallel, their enzyme inhibitory property against human neutrophil elastase was assessed. Four subfractions, essentially containing polyphenols and triterpenes, exhibited a significant elastase inhibitory activity and an ellagitannin showed a very high radical scavenging activity.

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Section: Resultsmentioning

confidence: 99%

“…Fractions of 20 mL were collected over the whole process. All fractions were analyzed by TLC and HPLC and then pooled to yield fractions EAF [1][2][3][4][5][6][7][8][9][10][11] .…”

Section: Centrifugal Partition Chromatographymentioning

confidence: 99%

Section: Hplc Purification Of Eafmentioning

confidence: 99%

mentioning

confidence: 99%

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Investigation of Antioxidant and Elastase Inhibitory Activities ofGeum urbanumAerial Parts, Chemical Characterization of Extracts Guided by Chemical and Biological Assays

Schmitt

Magid

Hubert

et al. 2020

Natural Product Communications

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“…The major classes of compounds found in the Fragaria species (anthocyanins and non-anthocyanin phenolic compounds) are known for their antioxidant properties [65,66]. As can be expected, the vast majority of the literature presenting the biological activities of species belonging to Fragaria genus present their antioxidant activity.…”

Section: Antioxidant Propertiesmentioning

confidence: 95%

Fragaria Genus: Chemical Composition and Biological Activities

et al. 2020

Self Cite

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The strawberries represent in our days one of the main fresh fruits consumed globally, inevitably leading to large amounts of by-products and wastes. Usually appreciated because of their specific flavor, the strawberries also possess biological properties, including antioxidant, antimicrobial, or anti-inflammatory effects. In spite of the wide spread of the Fragaria genus, few species represent the subject of the last decade scientific research. The main components identified in the Fragaria species are presented, as well as several biological properties, as emerging from the scientific papers published in the last decade.

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Strong insecticidal potential of methanol extract of Ferulago trifida fruits against Anopheles stephensi as malaria vector

Goodarzi

Tavakoli

Abai

et al. 2019

Environ Sci Pollut Res

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In vitro and in vivo evaluation of antioxidant properties of wild-growing plants. A short review

Cited by 42 publications

References 50 publications

Investigation of Antioxidant and Elastase Inhibitory Activities ofGeum urbanumAerial Parts, Chemical Characterization of Extracts Guided by Chemical and Biological Assays

Investigation of Antioxidant and Elastase Inhibitory Activities ofGeum urbanumAerial Parts, Chemical Characterization of Extracts Guided by Chemical and Biological Assays

Fragaria Genus: Chemical Composition and Biological Activities

Strong insecticidal potential of methanol extract of Ferulago trifida fruits against Anopheles stephensi as malaria vector

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