In vitro and in vivo inhibition of α-amylases of stored-product mite Acarus siro

Hubert, Jan; Dolečková-Marešová, Lucie; Hýblová, Jana; Kudlı́ková, Iva; Stejskal, V.; Mareš, Michael

doi:10.1007/s10493-004-7834-8

Cited by 28 publications

(25 citation statements)

References 24 publications

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“…Aca s 4 was inhibited by acarbose (IC 50 ~3.8 μM), a microbial oligosaccharide that is a general inhibitor of enzymes of the α-amylase class. This is in line with our previous finding that acarbose exerts an acaricidal activity against A. siro by inhibiting its digestive amylolytic activity [17]. Interestingly, Aca s 4 was insensitive to inhibition by two types of proteinaceous inhibitors of plant origin, namely wheat inhibitors WI-1 and WI-3 (tetrameric and monomeric form, respectively) and bean inhibitor αAI-1, which are potent inhibitors of various insect and mammalian α-amylases [20,21].…”

Section: Resultssupporting

confidence: 93%

“…The pH profile (Figure 4a) shows that the enzyme functions in the slightly acidic to neutral range, with a maximum at pH ~6.5, which is in accordance with the pH optimum of α-amylase activity measured with the whole body extract of A. siro [17]. A similar pH optimum was also reported for the purified Der p 4 [14].…”

Section: Resultssupporting

confidence: 84%

“…We conclude that there is an important difference in the distribution of digestive enzymes in these model species of storage and house dust mites, which most likely reflects their feeding ecology. The high level of α-amylase activity in A. siro is in accordance with the feeding preferences of A. siro , a granivorous species evolutionarily adapted to utilization of a starch-rich diet [17]. …”

Section: Resultsmentioning

confidence: 77%

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Enzymatic activity and immunoreactivity of Aca s 4, an alpha-amylase allergen from the storage mite Acarus siro

et al. 2012

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BackgroundEnzymatic allergens of storage mites that contaminate stored food products are poorly characterized. We describe biochemical and immunological properties of the native alpha-amylase allergen Aca s 4 from Acarus siro, a medically important storage mite.ResultsA. siro produced a high level of alpha-amylase activity attributed to Aca s 4. This enzyme was purified and identified by protein sequencing and LC-MS/MS analysis. Aca s 4 showed a distinct inhibition pattern and an unusual alpha-amylolytic activity with low sensitivity to activation by chloride ions. Homology modeling of Aca s 4 revealed a structural change in the chloride-binding site that may account for this activation pattern. Aca s 4 was recognized by IgE from house dust mite-sensitive patients, and potential epitopes for cross-reactivity with house dust mite group 4 allergens were found.ConclusionsWe present the first protein-level characterization of a group 4 allergen from storage mites. Due to its high production and IgE reactivity, Aca s 4 is potentially relevant to allergic hypersensitivity.

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Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 84%

Section: Resultsmentioning

confidence: 77%

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Enzymatic activity and immunoreactivity of Aca s 4, an alpha-amylase allergen from the storage mite Acarus siro

et al. 2012

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“…Due to this constraint, it was not possible to distinguish between lysozyme in the haemolymph or fat body and lysozyme in the gut using WME. The cells of the ventriculus and caeca of A. siro and T. putrescentiae showed a high degree of granularization and secretory activity, indicating the production of digestive enzymes (Hubert et al 2003(Hubert et al , 2005Smrz et al 1991;Smrz and Catska 1989). After secretion into the lumen of the ventriculus, the enzymes enter into a newly formed food bolus and hydrolyze the ingested substrates, passing together with undigested material through the gut and appearing finally in SGME.…”

Section: Discussionmentioning

confidence: 99%

Digestive function of lysozyme in synanthropic acaridid mites enables utilization of bacteria as a food source

Erban

Hubert

2008

Exp Appl Acarol

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The activity of lysozyme, the enzyme that hydrolyzes peptidoglycan in G(+) bacterial cell walls, was detected in whole mite extracts (WME) and in spent growth medium extracts (SGME) of 14 species of synanthropic mites (Acari: Acaridida). The adaptation of lysozyme for digestive activity and bacteriophagy was based on: (i) high lysozyme activity in SGME, and (ii) the correlation of maximum lysozyme activity at acidic pH values, corresponding to pH in the ventriculus and caeca. We show that the digestion of fluorescein-labeled Micrococcus lysodeikticus cells began in ventriculus and continued during the passage of a food bolus through the gut. The fluorescein was absorbed by midgut cells and penetrated to parenchymal tissues. Eight species showed a higher rate of population growth on a M. lysodeikticus diet than on a control diet. The lysozyme activity in SGME was positively correlated to the standardized rate (r (s)) of population growth, although no correlation was found between r (s) and lysozyme activity in WME. The lysozyme activity in WME was negatively correlated to that in SGME. The highest activity of digestive lysozyme was found in Lepidoglyphus destructor, Chortoglyphus arcuatus and Dermatophagoides farinae. All of these findings indicate that lysozyme in acaridid mites possesses both defensive and digestive functions. The enzymatic properties of mite lysozyme are similar to those of the lysozymes present in the ruminant stomach and in the insect midgut.

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“…The mites were mass-reared in plastic chambers derived from IWAKI tissue cell cultures with filter cap plugs (P-Lab, Praha, CZ). The chambers were kept in desiccators (P-Lab) with a saturated KCl solution providing 85% relative humidity and incubated at 25±1°C in the dark [21] Before the experiments, the mites were collected from the rearing chambers using a paint brush and a dissection Stemi 2000 C stereomicroscope (C. Zeiss, Jena, Germany).…”

Section: Methodsmentioning

confidence: 99%

The Effect of Antibiotics on Associated Bacterial Community of Stored Product Mites

et al. 2014

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BackgroundBacteria are associated with the gut, fat bodies and reproductive organs of stored product mites (Acari: Astigmata). The mites are pests due to the production of allergens. Addition of antibiotics to diets can help to characterize the association between mites and bacteria.Methodology and Principal FindingsAmpicillin, neomycin and streptomycin were added to the diets of mites and the effects on mite population growth (Acarus siro, Lepidoglyphus destructor and Tyrophagus putrescentiae) and associated bacterial community structure were assessed. Mites were treated by antibiotic supplementation (1 mgg−1 of diet) for 21 days and numbers of mites and bacterial communities were analyzed and compared to the untreated control. Bacterial quantities, determined by real-time PCR, significantly decreased in antibiotic treated specimens from 5 to 30 times in A. siro and T. putrescentiae, while no decline was observed in L. destructor. Streptomycin treatment eliminated Bartonella-like bacteria in the both A. siro and T. putrescentiae and Cardinium in T. putrescentiae. Solitalea-like bacteria proportion increased in the communities of neomycin and streptomycin treated A. siro specimens. Kocuria proportion increased in the bacterial communities of ampicillin and streptomycin treated A. siro and neomycin and streptomycin treated L. destructor.Conclusions/SignificanceThe work demonstrated the changes of mite associated bacterial community under antibiotic pressure in pests of medical importance. Pre-treatment of mites by 1 mgg−1 antibiotic diets improved mite fitness as indicated accelerated population growth of A. siro pretreated streptomycin and neomycin and L. destructor pretreated by neomycin. All tested antibiotics supplemented to diets caused the decrease of mite growth rate in comparison to the control diet.

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In vitro and in vivo inhibition of α-amylases of stored-product mite Acarus siro

Cited by 28 publications

References 24 publications

Enzymatic activity and immunoreactivity of Aca s 4, an alpha-amylase allergen from the storage mite Acarus siro

Enzymatic activity and immunoreactivity of Aca s 4, an alpha-amylase allergen from the storage mite Acarus siro

Digestive function of lysozyme in synanthropic acaridid mites enables utilization of bacteria as a food source

The Effect of Antibiotics on Associated Bacterial Community of Stored Product Mites

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