In vitro and in vivo behavior of DNA tetrahedrons as tumor-targeting nanocarriers for doxorubicin delivery

Kang, Ji Hee; Kim, Kyoung Ran; Lee, Hyukjin; Ahn, Dae Ro; Ko, Young Tag

doi:10.1016/j.colsurfb.2017.06.014

Cited by 38 publications

(33 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…They also did not stain for organelles besides the lysosomes (e.g., early endosomes), rendering it difficult for readers to address whether the DNA octahedra are truly in the cytosol or other organelles. In 2017, Kang et al prepared mirror l ‐TDNs of two different sizes and evaluated their localization inside HeLa cells by live imaging . While the smaller l ‐TDNs (with an edge length of 8.6 nm) are colocalized with the lysosomes starting from the first minute of incubation, the larger l ‐TDNs (with an edge length of 24.8 nm) appear in the lysosomes only after 30 min of incubation.…”

Section: Intracellular Fate Of Dna Nanostructuresmentioning

confidence: 99%

Progress toward Understanding the Interactions between DNA Nanostructures and the Cell

Chiu

Choi

2019

Small

View full text Add to dashboard Cite

Advances in DNA nanotechnology empower the programmable assembly of DNA building blocks (oligonucleotides and plasmids) into DNA nanostructures with precise architectural control. As DNA nanostructures are biocompatible and can naturally enter mammalian cells without the aid of transfection agents, they have found numerous biological or biomedical applications as delivery carriers of therapeutic and imaging cargoes into mammalian cells for at least a decade. Nevertheless, mechanistic studies on how DNA nanostructures interact with cells have remained limited and incomprehensive until 2–3 years ago. This Review presents the recent progress in elucidating the “cell–nano” interactions of DNA nanostructures, with an emphasis on three key classes of structures commonly utilized in intracellular applications: tile‐based structures, origami‐based structures, and nanoparticle‐templated structures. Structural parameters of DNA nanostructures and strategies of biochemical modification for promoting intracellular delivery are discussed. Biological mechanisms for cellular uptake, including specific pathways and receptors involved, are outlined. Routes of intracellular trafficking and degradation, together with strategies for re‐directing their trafficking, are delineated. This Review concludes with several aspects of the “bio–nano” interactions of DNA nanostructures that warrant future investigations.

show abstract

Section: Intracellular Fate Of Dna Nanostructuresmentioning

confidence: 99%

Progress toward Understanding the Interactions between DNA Nanostructures and the Cell

Chiu

Choi

2019

Small

View full text Add to dashboard Cite

show abstract

“…The same group constructed an L -DNA tetrahedron to deliver doxorubicin in vivo, finding decreased clearance and increased initial concentration (C0), half-life, and area under the curve (AUC). In addition, one with 17-mer per side showed higher internalization than that with 30-mer (Kang et al, 2017). They emphasizes the importance of DNA tetrahedron' s sizes and conformations when it is used for delivering drugs in vivo, provoking researchers into optimizing this carrier by altering the physicochemical properties, such as charge numbers and diverse modified groups.…”

Section: Small Moleculesmentioning

confidence: 99%

Development of DNA tetrahedron-based drug delivery system

Zhou

Zhang

et al. 2017

Drug Delivery

View full text Add to dashboard Cite

Nanocarriers of drugs have attracted significant attention to tackle the problems of drug resistance or nucleic acid drug delivery, which can optimize pharmaceutical parameters and enhance the cellular uptake efficiency. Nowadays, DNA nanostructure presents an opportunity in the field of nanomaterial due to its precise control in shape and size, excellent biocompatibility, as well as multiple sites for targeting decoration. DNA tetrahedron, which is stable and easily synthesized, is used for various applications, including nuclear magnetic resonance imaging, molecular diagnosis, targeting drug delivery, and so on. In this review, we will discuss the applications of DNA tetrahedron about drug delivery, intracellular routes and its fates. Also challenges and possible solutions for developing DNA tetrahedron-based drug delivery system are detailed. ARTICLE HISTORY

show abstract

“…Although the mechanisms of the cellular uptake of TDN are not well understood, 35 many studies reported that various DNA nanostructures can be taken up by cells without transfection reagents. It has been shown that smaller TDN was more rapidly internalized into cells than larger ones 36 . Walsh et al 31 studied the transfection efficiency of naked TDN and Lipofectin‐encapsulated TDN in mammalian cells.…”

Section: Cellular Uptake and Intracellular Fates Of Tdnmentioning

confidence: 99%

Tetrahedral DNA nanostructures as drug delivery and bioimaging platforms in cancer therapy

2020

View full text Add to dashboard Cite

Structural DNA nanotechnology enables DNA to be used as nanomaterials for novel nanostructure construction with unprecedented functionalities. Artificial DNA nanostructures can be designed and generated with precisely controlled features, resulting in its utility in bionanotechnological and biomedical applications. A tetrahedral DNA nanostructure (TDN), the most popular DNA nanostructure, with high stability and simple synthesis procedure, is a promising candidate as nanocarriers in drug delivery and bioimaging platforms, particularly in precision medicine as well as diagnosis for cancer therapy. Recent evidence collectively indicated that TDN successfully enhanced cancer therapeutic efficiency both in vitro and in vivo. Here, we summarize the development of TDN and highlight various aspects of TDN applications in cancer therapy based on previous reports, including anticancer drug loading, photodynamic therapy, therapeutic oligonucleotides, bioimaging platforms, and other molecules and discuss a perspective in opportunities and challenges for future TDN‐based nanomedicine.

show abstract

In vitro and in vivo behavior of DNA tetrahedrons as tumor-targeting nanocarriers for doxorubicin delivery

Cited by 38 publications

References 36 publications

Progress toward Understanding the Interactions between DNA Nanostructures and the Cell

Progress toward Understanding the Interactions between DNA Nanostructures and the Cell

Development of DNA tetrahedron-based drug delivery system

Tetrahedral DNA nanostructures as drug delivery and bioimaging platforms in cancer therapy

Contact Info

Product

Resources

About