In vitro and in vivo anti-inflammatory effects of different extracts from Epigynum auritum through down-regulation of NF-κB and MAPK signaling pathways

Yang, Meilian; Wang, Yudan; Patel, Gopal; Xue, Qingwang; Njateng, Guy Sedar Singor; Cai, Shengbao; Cheng, Guiguang; Kai, Guoyin

doi:10.1016/j.jep.2020.113105

Cited by 48 publications

(20 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The MAPKs signaling pathways, which are closely related to regulating of the above cytokines, 24 were detected by Western blotting. As shown in Figure 8 , the levels of phosphorylated ERK, JNK, and p38 MAPK were significantly enhanced by compounds stimulation, compared to that measured in the macrophages treated with LPS alone ( p <0.05).…”

Section: Resultsmentioning

confidence: 99%

PKCδ/MAPKs and NF-κB Pathways are Involved in the Regulation of Ingenane-Type Diterpenoids from Euphorbia neriifolia on Macrophage Function

Jiang

Xue

et al. 2021

JIR

View full text Add to dashboard Cite

Purpose Euphorbia neriifolia Linn. has important medicinal value in the treatment of ulcers, tumors, inflammation, chronic respiratory troubles, and so on. Although many ingredients with anti-inflammatory activity have been discovered and isolated from the Euphorbia neriifolia , the current research still cannot explain its multivariate effects on the immune response. This article aims to introduce two Ingenane-type diterpenoids from Euphorbia neriifolia with macrophage regulatory effects and to investigate the mechanism of their action. Methods The stem bark of E. neriifolia was extracted with various separation methods to obtain ingenane-type diterpenoids. The RAW264.7 cells were treated with lipopolysaccharide (LPS, 1 μg/mL) to establish an inflammatory cell model. The cell viability was detected by MTT assay. The secretion of PGE2, TNF-α, IL-1β, and IL-6 was tested with ELISA. The levels of iNOS, COX-2, IκBα, JNK, ERK, p38, p-IκBα, p-JNK, p-ERK, and p-p38 in cells were detected by Western blotting. The translocation of nuclear factor-kappa B (NF-κB)/p65 subunit were evaluated by Immunofluorescence staining. Results Ingenane-type diterpenoids, eurifoloid A (Euri A) and a new compound euphorneroid E (Euph E), were isolated from the EtOAc fraction of E. neriifolia stem bark extracts. Euph E and Euri A exhibited significant inhibition on the levels of pro-inflammatory mediators NO, IL-1β, IL-6, and iNOS on LPS-induced macrophage RAW264.7. Cellular signaling pathway studies showed that they prevented the degradation of IκBα and the translocation of NF-κB/p65 subunit. Furthermore, the production of PGE2, TNFα, and COX-2 was dramatically increased under the influence of the compounds, which were closely related to the phosphorylation of protein kinase C δ (PKCδ) and activation of mitogen-activated protein kinase (MAPKs) signaling pathway. Conclusion These results demonstrated that Euph E and Euri A exhibited multidirectional regulation on cytokines and immune function of macrophages, in addition to a good anti-inflammatory activity, and which was closely related to the regulation of PKCδ/MAPKs and NF-κB signal pathways.

show abstract

Section: Resultsmentioning

confidence: 99%

PKCδ/MAPKs and NF-κB Pathways are Involved in the Regulation of Ingenane-Type Diterpenoids from Euphorbia neriifolia on Macrophage Function

Jiang

Xue

et al. 2021

JIR

View full text Add to dashboard Cite

show abstract

“…LPS activates ERK1/2, JNK, and p38 MAPK, which ultimately control the activity of transcription factors regulating the expression of inflammation modulators, such as induced NO synthase (iNOS), cyclooxygenase-2 (COX-2), TNF-α, IL-1α, IL-1β, and IL-6 [ 44 ]. Inhibiting the activation of NF-κB and MAPK signalling pathways is an important treatment strategy for inflammatory diseases [ 15 , 45 ]. Many anti-inflammatory effects shown by polyphenols may be achieved by influencing the transcription network, regulating gene expression, changing the inflammatory cell cycle of recruitment, or homing to a certain extent, thereby eventually inhibiting the activation of NF-κB and MAPK signalling pathways [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Polyphenols Extracted from Shanxi-Aged Vinegar Inhibit Inflammation in LPS-Induced RAW264.7 Macrophages and ICR Mice via the Suppression of MAPK/NF-κB Pathway Activation

et al. 2021

View full text Add to dashboard Cite

Shanxi-aged vinegar, a traditional Chinese grain-fermented food that is rich in polyphenols, has been shown to have therapeutic effects on a variety of diseases. However, there has been no comprehensive evaluation of the anti-inflammatory activity of polyphenols extracted from Shanxi-aged vinegar (SAVEP) to date. The anti-inflammatory activities of SAVEP, both in RAW 264.7 macrophages and mice, were extensively investigated for the potential application of SAVEP as a novel anti-inflammatory agent. In order to confirm the notion that polyphenols could improve inflammatory symptoms, SAVEP was firstly detected by gas chromatography mass spectrometry (GC-MS). In total, 19 polyphenols were detected, including 12 phenolic acids. The study further investigated the protective effect of SAVEP on lipopolysaccharide-induced inflammation in RAW264.7 macrophages and ICR mice. The results showed that compared with those of the model group, SAVEP could remarkably recover the inflammation of macrophage RAW264.7 and ICR mice. SAVEP can normalise the expression of related proteins via the suppression of MAPK/NF-κB pathway activation, inhibiting the expression of iNOS and COX-2 proteins, and consequently the production of inflammatory factors, thus alleviating inflammatory stress. These results suggest that SAVEP may have a potential function against inflammation.

show abstract

“…After treatment, cells were collected and proteins were obtained according to the previous method. 29 The proteins were run in SDS-PAGE and then transferred to a polyvinylidene fluoride membrane. After incubation with primary antibody and corresponding secondary antibody, the proteins were identified by a gel image processing system (MF-ChemiBIS 2.0, DNR, Jerusalem, Israel).…”

Section: Methodsmentioning

confidence: 99%

Inhibitory effects of Atlantic cod (Gadus morhua) peptides on RANKL-induced osteoclastogenesis in vitro and osteoporosis in ovariectomized mice

Yang

Cheng

et al. 2022

Food Funct.

Self Cite

View full text Add to dashboard Cite

show abstract

In vitro and in vivo anti-inflammatory effects of different extracts from Epigynum auritum through down-regulation of NF-κB and MAPK signaling pathways

Cited by 48 publications

References 46 publications

PKCδ/MAPKs and NF-κB Pathways are Involved in the Regulation of Ingenane-Type Diterpenoids from Euphorbia neriifolia on Macrophage Function

PKCδ/MAPKs and NF-κB Pathways are Involved in the Regulation of Ingenane-Type Diterpenoids from Euphorbia neriifolia on Macrophage Function

Polyphenols Extracted from Shanxi-Aged Vinegar Inhibit Inflammation in LPS-Induced RAW264.7 Macrophages and ICR Mice via the Suppression of MAPK/NF-κB Pathway Activation

Inhibitory effects of Atlantic cod (Gadus morhua) peptides on RANKL-induced osteoclastogenesis in vitro and osteoporosis in ovariectomized mice

Contact Info

Product

Resources

About