In vitro and in vivo evaluation of effectiveness of a novel TEMPO-oxidized cellulose nanofiber-silk fibroin scaffold in wound healing

Shefa, Anha Afrin; Amirian, Jhaleh; Kang, Hoe Jin; Bae, Sang Ho; Jung, Hyun-Woo; Choi, Hwanjun; Lee, Sun Young; Lee, Byong-Taek

doi:10.1016/j.carbpol.2017.08.130

Cited by 107 publications

(58 citation statements)

References 88 publications

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“…NFC, the raw material used in this research, is typically produced from kraft bleached pulps using a mechanical and/or enzymatic treatment. The final nanofibrillated material has distinctive dimensions that range between 3 and 50 nm in diameter and several micrometers in length [6,8,[11][12][13][14]. Carboxyl groups can be introduced during the NFC's processing into the cellulosic backbone to improve delamination of the nanofibrils through 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO)-mediated oxidation [11][12][13]15].…”

Section: Introductionmentioning

confidence: 99%

“…The final nanofibrillated material has distinctive dimensions that range between 3 and 50 nm in diameter and several micrometers in length [6,8,[11][12][13][14]. Carboxyl groups can be introduced during the NFC's processing into the cellulosic backbone to improve delamination of the nanofibrils through 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO)-mediated oxidation [11][12][13]15]. The TEMPO radical is a free radical that selectively oxidizes the primary alcohol group of polysaccharides in the presence of NaBr/NaClO [11,13,15].…”

Section: Introductionmentioning

confidence: 99%

“…Carboxyl groups can be introduced during the NFC's processing into the cellulosic backbone to improve delamination of the nanofibrils through 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO)-mediated oxidation [11][12][13]15]. The TEMPO radical is a free radical that selectively oxidizes the primary alcohol group of polysaccharides in the presence of NaBr/NaClO [11,13,15]. The TEMPO/NaBr/NaClO treatment is applied to never-dried cellulose fibers prior to nanoscale processing to produce TEMPO-oxidized NFC (TNFC).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Acetaminophen Release from Biodegradable Poly (Vinyl Alcohol) (PVA) and Nanocellulose Films Using a Multiphase Release Mechanism

2020

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Biodegradable polymers hold great therapeutic value, especially through the addition of additives for controlled drug release. Nanocellulose has shown promise in drug delivery, yet usually requires chemical crosslinking with harsh acids and solvents. Nanocellulose fibrils (NFCs) and 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO)-mediated oxidized nanocellulose fibrils (TNFCs) with poly (vinyl alcohol) (PVA) could be aqueously formulated to control the release of model drug acetaminophen over 144 h. The release was evaluated with a multiphase release mechanism to determine which mechanism(s) contribute to the overall release and to what degree. Doing so indicated that the TNFCs in PVA control the release of acetaminophen more than NFCs in PVA. Modeling showed that this release was mostly due to burst release-drug coming off the immediate surface, rather than diffusing out of the matrix.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of Acetaminophen Release from Biodegradable Poly (Vinyl Alcohol) (PVA) and Nanocellulose Films Using a Multiphase Release Mechanism

2020

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“…For skin tissue engineering and wound healing, silk fibroin has been combined with various synthetic and natural polymers and other bioactive substances. The polymers included, for example, PCL, [67], poly(L-lactic acid)-co-poly(εcaprolactone) (PLACL, [68]), carboxyethyl chitosan, PVA, [69], chitin [70], cellulose-based materials modified by oxidation [71] or with lysozyme [72], collagen [73], gelatin [74], and hyaluronan [75]. The bioactive substances were, for example, growth factors, such as epidermal growth factor [64], vitamins, such as vitamin C [68], vitamin E [76], and pantothenic acid (vitamin B5; [77]), antioxidants, such as grape seed extract ( [78]) or quinone-based chromenopyrazole [79], antibiotics, such as ciprofloxacin [64], tetracycline [68] or gentamycin [62], and other antimicrobial and wound healing agents, such as silver nanoparticles, dandelion leaf extract [63], Aloe vera [80], or astragaloside IV [74].…”

Section: Introductionmentioning

confidence: 99%

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

Bačáková¹,

Pajorová²,

Zikmundová³

et al. 2020

Current and Future Aspects of Nanomedicine

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Nanofibrous scaffolds belong to the most suitable materials for tissue engineering, because they mimic the fibrous component of the natural extracellular matrix. This chapter is focused on the application of nanofibers in skin tissue engineering and wound healing, because the skin is the largest and vitally important organ in the human body. Nanofibrous meshes can serve as substrates for adhesion, growth and differentiation of skin and stem cells, and also as an antimicrobial and moistureretaining barrier. These meshes have been prepared from a wide range of synthetic and nature-derived polymers. This chapter is focused on the use of nature-derived polymers. These polymers have good or limited degradability in the human tissues, which depends on their origin and on the presence of appropriate enzymes in the human tissues. Non-degradable and less-degradable polymers are usually produced in bacteria, fungi, algae, plants or insects, and include, for example, cellulose, dextran, pullulan, alginate, pectin and silk fibroin. Well-degradable polymers are usually components of the extracellular matrix in the human body or at least in other verte brates, and include collagen, elastin, keratin and hyaluronic acid, although some polymers produced by non-vertebrate organisms, such as chitosan or poly(3-hydroxybutyrate-co-3-hydroxyvalerate), are also degradable in the human body.Current and Future Aspects of Nanomedicine 2 microbial infection, and at the same time, they can keep appropriate moisture and gas exchange at the wound site.Nanofibrous scaffolds for skin tissue engineering have been fabricated from a wide range of synthetic and nature-derived polymers, which can be either biostable or degradable within the human body. Biostable synthetic polymers used in nanofiber-based skin regenerative therapies include, for example, polyurethane [2], polydimethylsiloxane [3], polyethylene terephthalate [4], polyethersulfone [5], and also hydrogels such as poly(acrylic acid) (PAA, [6]), poly(methyl methacrylate) (PMMA, [7]), and poly[di(ethylene glycol) methyl ether methacrylate] (PDEGMA, [8]). Degradable synthetic polymers typically include poly(ε-caprolactone) (PCL, [9]) and its copolymers with polylactides (PLCL, [10]), polylactides (PLA, [11]) and their copolymers with polyglycolides (PLGA, [12]), and also so-called auxiliary polymers, such as poly(ethylene glycol) (PEG), poly(ethylene oxide) (PEO, [13]) or poly(vinyl alcohol) (PVA, [14]), which facilitated the electrospinning process and improved the mechanical properties and wettability of the chief polymer. However, the synthetic polymers, although they are well-chemically defined and tailorable, are often bioinert, hydrophobic and thus not promoting cell adhesion, and also not well-adhering to the wound site. Therefore, they need to be combined with other bioactive substances, particularly nature-derived polymers.This chapter is focused on nature-derived polymers used for fabrication of nanofibrous scaffolds for skin tissue engineering and wound healing. The advantages of...

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“…Full-thickness skin wounds in rats healed effectively under a film of BC nanocrystals/chitosan incorporating silver and curcumin particles [10]. BC scaffolds with curcumin particles encapsulated in chitosan and in composition with gelatin and fibrin were used to manage skin wounds in mice and rats [11][12][13]. Singla et al [14] investigated hydrogels prepared from bamboo cellulose nanocrystals loaded with silver particles, which successfully healed skin wounds in diabetic mice.…”

Section: Introductionmentioning

confidence: 99%

Biotechnological wound dressings based on bacterial cellulose and degradable copolymer P(3HB/4HB)

Volova

Shumilova

Nikolaeva

et al. 2019

International Journal of Biological Macromolecules

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Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)]a biodegradable polymer of microbial origin. Some of the experimental membranes were loaded with drugs promoting wound healing and epidermal cells differentiated from multipotent adipose-derived mesenchymal stem cells. A study has been carried out to investigate the structure and physical/mechanical properties of the membranes. The in vitro study showed that the most effective scaffolds for growing fibroblasts were composite BC/P(3HB/4HB) films loaded with actovegin. Two types of the experimental biotechnological wound dressings-BC/P(3HB/4HB)/actovegin and BC/P(3HB/ 4HB)/fibroblastswere tested in vivo, on laboratory animals with model third-degree skin burns. Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing. Experimental wound dressings promoted healing more effectively than VoskoPrana commercial wound dressing.

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In vitro and in vivo evaluation of effectiveness of a novel TEMPO-oxidized cellulose nanofiber-silk fibroin scaffold in wound healing

Cited by 107 publications

References 88 publications

Evaluation of Acetaminophen Release from Biodegradable Poly (Vinyl Alcohol) (PVA) and Nanocellulose Films Using a Multiphase Release Mechanism

Evaluation of Acetaminophen Release from Biodegradable Poly (Vinyl Alcohol) (PVA) and Nanocellulose Films Using a Multiphase Release Mechanism

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

Biotechnological wound dressings based on bacterial cellulose and degradable copolymer P(3HB/4HB)

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