In Vitro and In Vivo Cytotoxicities and Antileishmanial Activities of Thymol and Hemisynthetic Derivatives

Robledo, Sara M.; Osorio, Edison; Muñoz, Diego A.; Jaramillo, Luz; Restrepo, Alejandro Estrada; Arango, Gabriel Jaime Murillo; Vélez, Iván Darío

doi:10.1128/aac.49.4.1652-1655.2005

Cited by 88 publications

(61 citation statements)

References 17 publications

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“…This compound was the main constituent of oil sample 12 (L. micromera), as well as 16 and 17 (L. origanoides), and was also present in samples 14, 15 and 18 (L. origanoides), which were active against T. cruzi. Thymol was ineffective against intracellular amastigotes of L. chagasi infected THP-1 cells, consistent with what has been demonstrated on intracellular forms of L. (Viannia) panamensis (Robledo et al 2005).…”

Section: Resultssupporting

confidence: 72%

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Chemical composition and antiprotozoal activities of Colombian Lippia spp essential oils and their major components

Escobar

Pinto

Herrera

et al. 2010

Mem. Inst. Oswaldo Cruz

154

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Section: Resultssupporting

confidence: 72%

“…Although the former showed low antiprotozoal activity, the latter was the most active against promastigotes of L. chagasi. The activity of thymol derivatives has been demonstrated in vitro and on experimental models of Leishmania panamensis (Robledo et al 2005).…”

Section: Resultsmentioning

confidence: 99%

Chemical composition and antiprotozoal activities of Colombian Lippia spp essential oils and their major components

Escobar

Pinto

Herrera

et al. 2010

Mem. Inst. Oswaldo Cruz

154

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“…The literature reports both insecticide and bacteriostatic activity for citronellol (Oliveira-Filho et al, 2017;Robledo et al, 2005;Alviano et al, 2005;Park et al, 2012). Haida et al (2007) suggest that the essential oil obtained from the plant Cymbopogon citratus has citronellol as one of its chemical components, having antibacterial activity against some 20 bacterial species.…”

Section: Discussionmentioning

confidence: 99%

“…From among the many substances obtained from medicinal plants, linalool, citronellol, and thymol have all been cited in the literature for their antimicrobial activity (Robledo et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Susceptibility of cariogenic microorganisms to phytoconstituents

et al. 2018

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This study aimed to evaluate the in vitro antibacterial activity of the phytochemicals thymol, linalool, and citronellol against Streptococcus mutans, Streptococcus salivarius and Streptococcus oralis. Disk diffusion screening on solid medium and measurement of the diameter of the bacterial growth inhibition halos was the technique utilized. The Minimum Inhibitory Concentration (MIC) of the substances was determined using serial substance dilutions and microdilution technique in Brain Heart Infusion culture medium. After incubation for 24 hours in an oven at 37 °C, plate reading was completed and confirmed by visual method using 2,3,5 triphenyl tetrazolium chloride dye. The Minimum Bactericidal Concentration (MBC) was determined from MIC subcultures. Assays were performed in triplicate, and chlorhexidine was used as a positive control. The diameters in mm of the growth inhibition halos ranged between 7.3 and 10.7 for S. mutans, 7.3 and 10.0 for S. oralis, and 8.2 and 9.8 for S. salivarius. The MIC and MBC values obtained converged , ranging from maximum values in the presence of Linalool (1,250.0 mg/mL, 2,500.0 mg/mL and 2,500.0 mg/mL, respectively, for S. mutans, S. oralis, and S. salivarius); and minimum values with Thymol (312.5 μg/ml, 156.2 μg/mL and 156.2 μg/ml, respectively for S. mutans, S. oralis, and S. salivarius). All the tested phytochemicals displayed antibacterial activity, thus representing substances with potential applications in preventing tooth decay.Keywords: preventive dentistry, dental caries, citronellol, linalool, thymol. Susceptibilidade de microrganismos cariogênicos a fitoconstituintes ResumoEste estudo objetivou avaliar a atividade antibacteriana in vitro dos fitoquímicos timol, linalol e citronelol sobre Streptococcus mutans, Streptococcus salivaris e Streptococcus oralis. Utilizou-se a técnica de discos de difusão em meio sólido e medição do diâmetro dos halos de inibição. A concentração inibitória mínima (CIM) das substâncias foi determinada utilizando diluições em série das substâncias e técnica de microdiluição em meio de cultura de Brain Heart Infusion. Após incubação durante 24 horas em estufa a 37 °C, a leitura da placa foi confirmada pelo método visual usando o corante 2,3,5 trifenil cloreto de tetrazólio. A concentração bactericida mínima (CBM) foi determinada a partir de subculturas de MIC. Os ensaios foram realizados em triplicata, e clorexidina foi usada como um controle positivo. Os diâmetros dos halos de inibição do crescimento variaram entre 7,3 e 10,7 por S. mutans, 7,3 e 10,0 por S. oralis, e 8,2 e 9,8 para S. salivaris. Os valores de CIM e CBM obtidos variaram de valores máximos na presença de linalol (1.250,0 mg/mL, 2.500.0 mg/mL e 2.500.0 mg/mL, respectivamente, para o S. mutans, S oralis e S. salivaris); a valores mínimos com timol (312,5 μg/ml, 156,2 μg/mL e 156,2 μg

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“…Carvacrol은 항염증제, 항산화제, 간장 보호 및 항종양제 등 여러 생물학적 작용을 가지고 있고 (Aeschbach et al, 1994;Weber and de Bont, 1996;Skold et al, 1998;Alam et al, 1999;Zeytinoglu et al, 2003;Robledo et al, 2005), 최근 연구에 의하면 치아우식증의 원인균인 S. mutans의 성장을 억제하는 항균력을 가지고 있는 것으로 보고되 었다 (Botelho et al, 2007) Carvacrol이 NHGF 세포의 생존에 미치는 영향을 알아보기 위해 MTT 분석법을 실시한 결과 carvacrol 128 µg/ml 농도에서 는 0.3%의 세포생존율을 보였고, 64 µg/ml에서는 50.8%의 생존 율을 보였다 (Fig. 1).…”

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Antimicrobial Effect of Carvacrol against Cariogenic and Periodontopathic Bacteria

Park¹,

Lee²,

Lim³

et al. 2012

The Korean Journal of Microbiology

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The aim of this study was to evaluate the antimicrobial effect of carvacrol against periodontopathic and cariogenic bacteria and its cytotoxicity in human oral tissue cells. We tested their antibacterial properties against mutans streptococci and five major periodontopathic bacterial species involved in periodontal disease. The antimicrobial activity was evaluated by the minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The cell viability of carvacrol on normal human gingival fibroblast (NHGF) cells was tested by metyl thiazolyl tetrazolium assay. The data showed that carvacrol had remarkable antimicrobial effect on tested bacteria with a MIC and MBC values ranged from 16 to 128 µg/ml and from 32 to 128 µg/ml, respectively. In cell toxicity studies, carvacrol had significantly decreased cell viability when NHGF cells were treated at 128 µg/ml. These findings suggest that carvacrol has a strong antimicrobial activity against periodontopathic and cariogenic bacteria. However, in order to use it as a component of gargling solution or toothpaste, its concentration should be below 64 µg/ml and other compounds having an antimicrobial activity against periodontopathic and cariogenic bacteria should be used together.

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In Vitro and In Vivo Cytotoxicities and Antileishmanial Activities of Thymol and Hemisynthetic Derivatives

Cited by 88 publications

References 17 publications

Chemical composition and antiprotozoal activities of Colombian Lippia spp essential oils and their major components

Chemical composition and antiprotozoal activities of Colombian Lippia spp essential oils and their major components

Susceptibility of cariogenic microorganisms to phytoconstituents

Antimicrobial Effect of Carvacrol against Cariogenic and Periodontopathic Bacteria

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