In Vitro and In Vivo Gene Delivery by Recombinant Baculoviruses

Tani, Hideki; Lim, Chang‐Kweng; Yap, Chan Choo; Onishi, Misa; Nozaki, Masami; Nishimune, Yoshitake; Okahashi, Nobuo; Kitagawa, Yuichi; Watanabe, Rie; Mochizuki, Rika; Moriishi, Kohji; Matsuura, Yoshiharu

doi:10.1128/jvi.77.18.9799-9808.2003

Cited by 172 publications

(152 citation statements)

References 42 publications

(57 reference statements)

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“…The use of polyspecific EcTyrRS and MbPylRS facilitates the incorporation of several useful UAAs using a single suppressor virus, including UAAs with bioorthogonal handles for chemical conjugation, photoaffinity labels, and others. Baculovirus, especially the pseudotyped version, has been shown to efficiently transduce a wide range of mammalian cells both in vivo and in vitro, all of which now should be suitable hosts for UAA mutagenesis of target proteins (9)(10)(11)(12)(13)(14)(15). The unique baculovirus vectors developed in this study should also be useful for other applications, where multiple genetic elements need to be simultaneously delivered into mammalian cells.…”

Section: Resultsmentioning

confidence: 99%

“…This strategy has been successfully applied to baculovirus using vesicular stomatitis virus G glycoprotein, VSVG (11)(12)(13)(14). Therefore, we constructed the shuttle vector pAcBac1 (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…AcNPV is also able to infect some mammalian cells, where its genetic elements remain silent rendering it replication incompetent. Thus, it can be safely used to deliver genetic cargo to a variety of different mammalian cell types both in vitro and in vivo (9)(10)(11)(12)(13)(14)(15). Several properties of baculovirus make it attractive as a potential delivery vector for the UAA incorporation machinery, including its very large cargo capacity (>30 kb), stable double-stranded DNA genome, broad host-tropism, ease of production, long shelf-life of the purified virus, intrinsically safe nature, and minimal cytotoxicity to mammalian cells, even when high multiplicity of infection (MOI) is used (9)(10)(11)(12)(13)(14)(15).…”

Section: Resultsmentioning

confidence: 99%

“…A UAAs incorporated using OMeYRS (1-9) and MbPylRS (10)(11)(12)(13)(14) B Incorporation of 2 in GFP* in various cells Incorporation of pAcF 2 in eGFP (Tyr39TAG) using pAcBac1.tR4-OMeYRS baculovirus in various cells. Cells were infected with the suppressor and the reporter virus at an optimal 1:6 ratio (MOI of 800) and were analyzed 48 h postinfection.…”

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confidence: 99%

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Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells

Chatterjee

Xiao

Bollong

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

101

132

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Here we report the development of a baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells. We have exploited the large cargo-capacity (>30 kb) and stability of the double-stranded DNA genome of baculovirus to deliver to a variety of cell types all of the components required to genetically incorporate novel amino acids. These include the engineered tRNA/aminoacyl-tRNA synthetase pair and the nonsense mutant of the target gene. Mammalian cell transduction efficiency of baculovirus was significantly improved by incorporating genetic elements from mammalian viruses. Two polyspecific tRNA/aminoacyl-tRNA synthetase pairs were inserted into this expression system, enabling the site-specific incorporation of a variety of unnatural amino acids with novel chemical and biological properties into proteins.genetic code | viral vector | nonstandard amino acid | orthogonal

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells

Chatterjee

Xiao

Bollong

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

101

132

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“…They have been shown to transduce various cell types in vitro and in vivo with significant efficiency. [2][3][4][5] However, efficient gene delivery still remains a challenge for baculovirus as for other gene therapy vectors in many target cells. Efficient transduction would allow high transgene expression with a lower viral load.…”

Section: Introductionmentioning

confidence: 99%

Truncated vesicular stomatitis virus G protein improves baculovirus transduction efficiency in vitro and in vivo

et al. 2005

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Baculovirus Expression Systems

Possee¹,

Hitchman²,

King³

2010

Encyclopedia of Industrial Biotechnology

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Baculoviruses are insect‐specific pathogens, producing occlusion bodies containing enveloped virus particles which spread infection between insects. These occlusions or polyhedra are composed mainly of a 30 KDa virus‐encoded protein produced in the very late phase of virus replication. Polyhedrin protein is not required for virus replication in cell culture and so the gene coding region can be replaced with a foreign sequence of choice to generate a baculovirus expression vector. The inserted coding region is placed under the transcriptional control of the polyhedrin gene promoter by transfection of insect cells with circular or linear baculovirus DNA or via transposition in bacteria. The coding region can be modified to allow the addition of different secretion signal peptides or peptide tags or multiple gene promoters can be used for the expression of more than one protein. The baculovirus expression vector may also be modified by the removal of nonessential genes encoding products deleterious to recombinant protein production. High yields of protein are possible by scaling up insect cell cultures in serum‐free medium. Despite their specificity for insect cells, baculoviruses can also be use to transduce human cells, where recombinant genes can be expressed if placed under the control of mammalian‐specific gene promoters.

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In Vitro and In Vivo Gene Delivery by Recombinant Baculoviruses

Cited by 172 publications

References 42 publications

Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells

Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells

Truncated vesicular stomatitis virus G protein improves baculovirus transduction efficiency in vitro and in vivo

Baculovirus Expression Systems

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