In vitro and in vivo survival of frozen-thawed bovine oocytes after IVF, nuclear transfer, and parthenogenetic activation

Kubota, Chikara; Yang, Xiangzhong; Dinnyés, András; Todoroki, Junichi; Yamakuchi, Hiroshi; Mizoshita, Kazunori; Inohae, Satoru; Tabara, Norio

doi:10.1002/(sici)1098-2795(199811)51:3<281::aid-mrd7>3.0.co;2-l

Cited by 66 publications

(22 citation statements)

References 27 publications

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“…Moreover, the percentages of maturation of vitrified‐thawed immature DOs were generally higher than previous data reported in cow either on vitrification or slow freezing of immature cumulus‐enclosed oocytes (Hochi et al. 1998; Kubota et al. 1998; Le Gal and Massip 1999; Hurtt et al.…”

Section: Discussioncontrasting

confidence: 78%

“…Slow freezing and thawing were carried out as previously described (Kubota et al. 1998) with minor modifications.…”

Section: Methodsmentioning

confidence: 99%

“…Cryopreservation of immature oocytes might avoid this problem, since genetic material is enclosed within the nuclear envelope (Suzuki et al. 1996; Kubota et al. 1998; Matsumoto et al.…”

Section: Introductionmentioning

confidence: 99%

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Cryopreservation of Immature Bovine Oocytes to Reconstruct Artificial Gametes by Germinal Vesicle Transplantation

Luciano

Franciosi

Lodde

et al. 2009

Reprod Domestic Animals

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Joining immature gamete cryopreservation and germinal vesicle transplantation (GVT) technique could greatly improve assisted reproductive technologies in animal breeding and human medicine. The present work was aimed to assess the most suitable cryopreservation protocol between slow freezing and vitrification for immature denuded bovine oocytes, able to preserve both nuclear and cytoplasmic competence after thawing. In addition, the outcome of germinal vesicle transfer procedure and gamete reconstruction was tested on the most effective cryopreservation system. Oocytes, isolated from slaughterhouse ovaries, were stored after cumulus cells removal either by slow freezing or by vitrification in open pulled straws. After thawing, oocytes were matured for 24 h in co-culture with an equal number of just isolated intact cumulus enclosed oocytes, and fixed in order to evaluate the stage of meiotic progression and cytoskeleton organization. Our results showed that after warming, vitrified oocytes reached metaphase II (MII) in a percentage significantly higher than oocytes cryopreserved by slow freezing (76.2% and 36.5% respectively, p < 0.05). Moreover, vitrification process preserved the organization of cytoskeleton elements in a higher proportion of oocytes than slow freezing procedure. Therefore vitrification has been identified as the elective method for denuded immature oocytes banking and it has been applied in the second part of the study. Our results showed that 38.3% of oocytes reconstructed from vitrified gametes reached the MII of meiotic division, with efficiency not different from oocytes reconstructed with fresh gametes. We conclude that vitrification represents a suitable method of GV stage denuded oocyte banking since both nuclear and cytoplasmic components derived from cryopreserved immature oocytes can be utilized for GVT.

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Section: Discussioncontrasting

confidence: 78%

“…Slow freezing and thawing were carried out as previously described (Kubota et al. 1998) with minor modifications.…”

Section: Methodsmentioning

confidence: 99%

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Cryopreservation of Immature Bovine Oocytes to Reconstruct Artificial Gametes by Germinal Vesicle Transplantation

Luciano

Franciosi

Lodde

et al. 2009

Reprod Domestic Animals

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“…In other species, such as bovine, the birth rate ranged from 0.6% to 0.8% (Suzuki et al. 1996; Kubota et al. 1998; Vieira et al.…”

Section: Introductionmentioning

confidence: 99%

Effects of Cryopreservation on the Meiotic Spindle, Cortical Granule Distribution and Development of Rabbit Oocytes

Jiménez-Trigos¹,

Naturil-Alfonso²,

Vicente³

et al. 2011

Reprod Domestic Animals

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Although much progress has been made in oocyte cryopreservation since 1971, live offspring have only been obtained in a few species and in rabbits. The aim of our study was to evaluate the effect of vitrification and slow freezing on the meiotic spindle, cortical granule (CG) distribution and their developmental competence. Oocytes were vitrified in 16.84% ethylene glycol, 12.86% formamide, 22.3% dimethyl sulphoxide, 7% PVP and 1% of synthetic ice blockers using Cryotop as device or slow freezing in 1.5 m PROH and 0.2 m sucrose in 0.25 ml sterile French mini straws. Meiotic spindle and CG distribution were assessed using a confocal laser-scanning microscope. To determine oocyte competence, in vitro development of oocytes from each cryopreservation procedure was assessed using parthenogenesis activation. Our data showed that oocytes were significantly affected by both cryopreservation procedures. In particular, meiotic spindle organization was dramatically altered after cryopreservation. Oocytes with peripheral CG distribution have a better chance of survival in cryopreservation after slow-freezing procedures compared to vitrification. In addition, slow freezing of oocytes led to higher cleavage and blastocyst rates compared to vitrification. Our data showed that, in rabbits, structural alterations are more evident in vitrified oocytes than in slow-frozen oocytes, probably as a consequence of sensitivity to high levels of cryoprotectants. Slow-freezing method is currently the recommended option for rabbit oocyte cryopreservation.

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“…However, the fertilization rate reduced when hamster oocytes were vitrified in vitrification solution containing 15% FCS , which suggested that the protective capacity of FCS may vary among species or batches. It was reported that the relatively high survival rate and blastocyst rate of vitrified-thawed oocytes were mainly attributed to the increased cooling/warming rates during freezing and thawing (Kubota et al 1998). A minimum amount of vitrification solution plunged directly into liquid nitrogen achieved high cooling/warming rates and reduced the occurrence of cracking (Vajta et al 1997).…”

Section: Discussionmentioning

confidence: 99%

Factors affecting the survival, fertilization, and embryonic development of mouse oocytes after vitrification using glass capillaries

Tan

Song

Liu

et al. 2009

In Vitro Cell.Dev.Biol.-Animal

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Cryopreservation of mammalian oocytes is an important way to provide a steady source of materials for research and practice of parthenogenetic activation, in vitro fertilization, and nuclear transfer. However, oocytes cryopreservation has not been common used, as there still are some problems waiting to be solved on the repeatability, safety, and validity. Then, it is necessary to investigate the damage occurred from vitrification and find a way to avoid or repair it. In this study, mouse mature oocytes were firstly pretreated in different equilibrium media, such as 5% ethylene glycol (EG) + 5% dimethyl sulfoxide (DMSO), 10% EG + 10% DMSO, and 15% EG + 15% DMSO in TCM199 supplemented with 20% fetal calf serum (FCS), for 1, 3, and 5 min, respectively, and then oocytes were transferred into vitrification solution (20% EG, 20% DMSO, 0.3 M sucrose, and 20% FCS in TCM199, M2, Dulbecco's phosphate buffered saline, and 0.9% saline medium, respectively) and immediately loaded into glass capillaries to be plunged into liquid nitrogen. After storage from 1 h to 1 wk, they were diluted in stepwise sucrose solutions. The surviving oocytes were stained for cortical granule, meiotic spindles, and chromosomes. Oocytes without treatments were used as controls. The results showed that oocytes pretreated in 5% EG +5% DMSO group for 3-5 min or in 10% EG + 10% DMSO group for 1-3 min were better than other treatments. Oocytes vitrified in TCM199 as basic medium showed higher survival and better subsequent embryonic development than other groups. When the concentration of FCS in vitrification solution reduced below 15%, the rates of survival, fertilization, and developing to blastocyst declined dramatically. The inner diameter (0.6 mm) of glass capillaries and amount of vitrification solution (1-3 microl) achieved more rapid cooling and warming and so reduce the injury to oocytes. Cropreservation led to the exocytosis of cortical granule of oocytes (about 10%) and serious disturbance of microtubules and chromosomes. With 2 h incubation, the microtubules could repolymerize and the rate of fertilization in vitro was much higher than those of 1 and 3 h incubation groups. In conclusion, the protection of basic medium and FCS to oocytes during cryopreservation and sufficient cooling and warming rates using glass capillaries have profound effects on oocytes survival and subsequent embryonic development competence. The appropriate time for fertilization in vitro may be related to the recovery of spindles after incubation and avoiding ageing in the whole process.

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In vitro and in vivo survival of frozen-thawed bovine oocytes after IVF, nuclear transfer, and parthenogenetic activation

Cited by 66 publications

References 27 publications

Cryopreservation of Immature Bovine Oocytes to Reconstruct Artificial Gametes by Germinal Vesicle Transplantation

Cryopreservation of Immature Bovine Oocytes to Reconstruct Artificial Gametes by Germinal Vesicle Transplantation

Effects of Cryopreservation on the Meiotic Spindle, Cortical Granule Distribution and Development of Rabbit Oocytes

Factors affecting the survival, fertilization, and embryonic development of mouse oocytes after vitrification using glass capillaries

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