In Vitro and In Vivo Photothermal Cancer Therapeutic Effects of Gold Nanorods Modified with Mushroom β-Glucan

Li, Xiaojie; Zhou, Jiajing; Dong, Xiaonan; Cheng, Wai-Yin; Duan, Hongwei; Cheung, Peter Chi Keung

doi:10.1021/acs.jafc.8b00292

Cited by 43 publications

(22 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, the classic cetyltrimethylammonium bromide-mediated synthesis approach was used for the preparation of Au nanorods (ARs) (28,29), and uniform ARs with an aspect ratio of 7.1 (length 106.4 ± 14.1 nm; width 15.2 ± 3.3 nm) were obtained (SI Appendix, Fig. S1).…”

Section: Resultsmentioning

confidence: 99%

Near-infrared optogenetic engineering of photothermal nanoCRISPR for programmable genome editing

Chen

Xin

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

146

154

View full text Add to dashboard Cite

We herein report an optogenetically activatable CRISPR-Cas9 nanosystem for programmable genome editing in the second near-infrared (NIR-II) optical window. The nanosystem, termed nanoCRISPR, is composed of a cationic polymer-coated Au nanorod (APC) and Cas9 plasmid driven by a heat-inducible promoter. The APC not only serves as a carrier for intracellular plasmid delivery but also can harvest external NIR-II photonic energy and convert it into local heat to induce the gene expression of the Cas9 endonuclease. Due to high transfection activity, the APC shows strong ability to induce a significant level of disruption in different genomic loci upon optogenetic activation. Moreover, the precise control of genome-editing activity can be simply programmed by finely tuning exposure time and irradiation time in vitro and in vivo and also enables editing at multiple time points, thus proving the sensitivity and inducibility of such an editing modality. The NIR-II optical feature of nanoCRISPR enables therapeutic genome editing at deep tissue, by which treatment of deep tumor and rescue of fulminant hepatic failure are demonstrated as proof-of-concept therapeutic examples. Importantly, this modality of optogenetic genome editing can significantly minimize the off-target effect of CRISPR-Cas9 in most potential off-target sites. The optogenetically activatable CRISPR-Cas9 nanosystem we have developed offers a useful tool to expand the current applications of CRISPR-Cas9, and also defines a programmable genome-editing strategy toward high precision and spatial specificity.

show abstract

Section: Resultsmentioning

confidence: 99%

Near-infrared optogenetic engineering of photothermal nanoCRISPR for programmable genome editing

Chen

Xin

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

146

154

View full text Add to dashboard Cite

show abstract

“…The intrinsic (without NIR radiation) and NIR‐induced toxicities of the synthesized RhNPs were evaluated through a cell viability assay based on reduction of the reagent thiazoyl blue tetrazolium bromide (MTT). We selected the applied NIR radiation (800 nm, 2.5 W cm −2 , and 10 min) based on typical values used in various previously published studies with metallic nanoparticles of similar sizes …”

Section: Resultsmentioning

confidence: 99%

Rhodium Nanoparticles as a Novel Photosensitizing Agent in Photodynamic Therapy against Cancer

Machuca

García-Calvo

Anunciação

et al. 2020

Chemistry A European J

View full text Add to dashboard Cite

Photodynamic therapy (PDT) is a promising alternative treatment for different types of cancer due to its high selectivity, which prevents healthy tissues from being damaged. The use of nanomaterials in PDT has several advantages over classical photosensitizing agents, due to their unique properties and their capacity for functionalization. Especially interesting is the use of metallic nanoparticles, which are capable of absorbing electromagnetic radiation and either transferring this energy to oxygen molecules for the generation of reactive oxygen species (ROS) or dissipating it as heat. Although previous reports have demonstrated the capacity of Rh derivatives to serve as anti‐tumor drugs, to the best of our knowledge there have been no studies on the potential use of small‐sized Rh nanoparticles as photosensitizers in PDT. In this study, 5 nm Rh nanoparticles have been synthesized and their potential in PDT has been evaluated. The results show that treatment with Rh nanoparticles followed by NIR irradiation induces apoptosis in cancer cells through a p53‐independent mechanism.

show abstract

“…In our study, the classic cetyltrimethylammonium bromide (CTAB)-mediated synthesis approach was used for the preparation of gold nanorods 38,39 , and uniform ARs with an aspect ratio of 7.1 (length = 106.4 ± 14.1 nm, width = 15.2 ± 3.3 nm) were obtained (Fig. 1a).…”

Section: Resultsmentioning

confidence: 99%

“…ARs were synthesized using a seed-mediated growth method as previously described 38,39 with minor modification. Briefly, the seed solution was first synthesized by adding freshly prepared 600 μL NaBH 4 (10 mM) into 5 mL mixture of HAuCl 4 ·3H 2 O (0.5 mM) and CTAB (0.1 M), followed by being kept at 30 °C for 30 min to exhaust excess NaBH 4 .…”

Section: Methodsmentioning

confidence: 99%

Optogenetic Control of Programmable Genome Editing by Photoactivatable CRISPR/Cas9 Nanosystem in the Second Near-Infrared Window

Chen

Xin

et al. 2019

Preprint

View full text Add to dashboard Cite

53We herein report the first optogenetically activatable CRISPR/Cas9 nanosystem for 54 programmable genome editing in the second near-infrared (NIR-II) optical window. The 55 nanosystem is composed of a cationic polymer-coated gold nanorod (APC) and Cas9 56 plasmid driven by a heat-inducible promoter. APC not only serves as a carrier for 57 intracellular plasmid delivery, but also can harvest external NIR-II photonic energy and 58 convert into local heat to induce the gene expression of Cas9 endonuclease. Due to high 59 transfection activity, APC shows strong ability to induce significant level of disruption in 60 different genome loci upon optogenetic activation. Moreover, the precise control of 61 genome editing activity can be simply programmed by finely tuning exposure time and 62 irradiation times in vitro and in vivo, and also enables editing at multiple time points, thus 63 proving the sensitivity and reversibility of such an editing modality. The NIR-II optical 64 feature of APC enables therapeutic genome editing at the deep tissue of the tumor-65 bearing mice, by which tumor growth could be effectively inhibited as a proof-of-concept 66 therapeutic example. Importantly, this modality of optogenetic genome editing can 67 significantly minimize the off-target effect of CRISPR/Cas9 in the most potential off-target 68 sites. The optogenetically activatable CRISPR/Cas9 nanosystem we have developed 69 offers a useful tool to expand the current applications of CRISPR/Cas9, and also defines 70 a programmable genome editing strategy towards unprecedented precision and 71 spatiotemporal specificity. 72 Introduction 73The RNA-guided clustered, regularly interspaced, short palindromic repeats (CRISPR)-74 associated nuclease protein 9 (Cas9) is originally an adaptive immune defense system, 75 by which many bacteria exploit to protect themselves from invading genetic elements 1 . It 76 has been recently harnessed as an efficient tool for genome editing in both single cells 77 and the whole organism for a wide range of biomedical applications in biology, genetics, 78 and medicine, etc 2,3 . In principle, the CRISPR/Cas9 is composed a single-guide RNA 79 (sgRNA) for the identification of DNA targets and a Cas9 endonuclease that can bind 80 and process the recognized DNA targets 4 . CRISPR/Cas9-based genome editing 81 technology offers a powerful and reliable strategy for the targeted modifications of the 82 genome, enabling the precise perturbation of virtually any genomic sequence in living 83 cells 2-6 . Due to its genome-wide specificity and multiplexing capability, Cas9 and its 84 variants have shown great potentials in the generation of loss-of-function animals 7,8 , the 85 correction of genetic disorders 9,10 , functional genome screening [11][12][13][14][15] , and the treatment of 86 infectious diseases 16,17 . Despite of these excitements, the lack of temporal and spatial 87 precision during editing process has severely constrained the current CRISPR/Cas9 88 systems from complicated and diverse genome-editing s...

show abstract

In Vitro and In Vivo Photothermal Cancer Therapeutic Effects of Gold Nanorods Modified with Mushroom β-Glucan

Cited by 43 publications

References 39 publications

Near-infrared optogenetic engineering of photothermal nanoCRISPR for programmable genome editing

Near-infrared optogenetic engineering of photothermal nanoCRISPR for programmable genome editing

Rhodium Nanoparticles as a Novel Photosensitizing Agent in Photodynamic Therapy against Cancer

Optogenetic Control of Programmable Genome Editing by Photoactivatable CRISPR/Cas9 Nanosystem in the Second Near-Infrared Window

Contact Info

Product

Resources

About