In vitro and in vivo trans-epidermal water loss evaluation following topical drug delivery systems application for pharmaceutical analysis

Cristiano, Maria Chiara; Froiio, Francesca; Mancuso, Antonia; Iannone, Michelangelo; Fresta, Massimo; Fiorito, Serena; Celia, Christian; Paolino, Donatella

doi:10.1016/j.jpba.2020.113295

Cited by 28 publications

(19 citation statements)

References 23 publications

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“…In case of skin barrier disruption, the amount of water evaporating through the epidermis will increase, therefore increasing the TEWL values. Thus, this parameter can be used as a standard to measure the integrity of skin barrier [14,15].…”

Section: Introductionmentioning

confidence: 99%

Advanced Characterization of Imiquimod-Induced Psoriasis-Like Mouse Model

et al. 2020

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Many autoimmune disorders such as psoriasis lead to the alteration of skin components which generally manifests as unwanted topical symptoms. One of the most widely approved psoriasis-like animal models is the imiquimod (IMQ)-induced mouse model. This representation mimics various aspects of the complex cutaneous pathology and could be appropriate for testing topical treatment options. We perform a thorough characterization of this model by assessing some parameters that are not fully described in the literature, namely a precise description of skin disruption. It was evaluated by transepidermal water loss measurements and analyses of epidermis swelling as a consequence of keratinocyte hyperproliferation. The extent of neo-angiogenesis and hypervascularity in dermis were highlighted by immunostaining. Moreover, we investigated systemic inflammation through cytokines levels, spleen swelling and germinal centers appearance in draining lymph nodes. The severity of all parameters was correlated to IMQ concentration in skin samples. This study outlines new parameters of interest useful to assess this model. We highlight the skin barrier disruption and report a systemic inflammatory reaction occurring at distance both in spleen and lymph nodes. These newly identified biological endpoints could be exploited to investigate the efficacy of therapeutic candidates for psoriasis and more extensively for several other skin inflammatory diseases.

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Section: Introductionmentioning

confidence: 99%

Advanced Characterization of Imiquimod-Induced Psoriasis-Like Mouse Model

et al. 2020

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“…In fact, Celia et al [ 41 ] affirmed that only a variation of backscattering profiles greater than 10% is indicative for the presence of segregation phenomena. The variation in ∆t and ∆bs profiles that occur below 2 mm and over 8 mm did not depend on destabilization of ufasomes, but it was due to air inclusion in the bottom and/or the top of Turbiscan Lab ® vials [ 30 ]. These results confirmed the stability of the systems previously provided on the basis of zeta-potential values.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, Turbiscan Stability Index (TSI) profiles versus time were used to compare the different formulation in terms of stability. The measurements were performed at 24 ± 1 °C for 1 h [ 30 ]. TSI is a statistical parameter obtained using the following equation:

where x i is the mean backscattering for each minute of measurements; x bs is mean of x i ; and n is the number of scans [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

Oleuropein-Laded Ufasomes Improve the Nutraceutical Efficacy

et al. 2021

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Ufasomes are unsaturated fatty acid liposomes made up of oleic and linoleic acids, natural components required in various biological processes. This kind of nanocarrier is characterized by a simple and dynamic structure and is able to improve the bioavailability of unsaturated fatty acids. The aim of this investigation was to evaluate ufasomes as natural compound delivery systems to deliver oleuropein and improve its antioxidant activity. Oleuropein is a phenolic compound mainly present in olives and olive oil, with several biological properties, such as the antioxidant activity. However, to improve their biological activity, antioxidant compounds should be able to cross cell membranes and uniformly incorporate in cells. Because of the great similarity between their constituents and cell membranes, ufasomes could be advantageous carriers for oleuropein delivery. The physico-chemical characteristics of ufasomes were investigated. A regular shape was shown by transmission electron microscopy studies, while the mean sizes were dependent on the ufasomes composition. In vitro studies highlighted that empty ufasomes did not lead to cell mortality at the tested concentrations and a good carrier internalization in CaCo-2 cells, further studies in vitro studies demonstrated that oleuropein-loaded ufasomes were able to enhance the antioxidant activity of the free active substance making this carrier a suitable one for nutraceutical application.

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“…The study showed that an initial interaction between carriers and skin structures occurred to allow the topical delivery of drugs, but without inducing irreversible modifications. 22 The carriers analyzed in that study are among the most used drug delivery systems involved in the treatment of acne disease.…”

Section: Topical Drug Delivery Systems To Overcome the Limits Of Convmentioning

confidence: 99%

“…They probably fuse with the skin surface, enhancing the permeation of drugs through the stratum corneum, owing to their ability to temporarily modify the structure of this barrier. 22,47 Because of their apparent advantages, especially in terms of carrier non-toxicity, the potential of niosomes in the treatment of skin diseases, such as acne, was investigated. A comparison study between retinoic acid-loaded liposomes and niosomes was performed to investigate the influence of nanovesicle composition on their cutaneous delivery ability.…”

Section: Niosomesmentioning

confidence: 99%