In vitro and In vivo Anti-Helicobacter pylori Activities of Centella asiatica Leaf Extract

Abstract

Helicobacter pylori infection is associated with an increased risk of developing upper gastrointestinal tract diseases. However, treatment failure is a major cause of concern mainly due to possible recurrence of infection, the side effects, and resistance to antibiotics. The aim of this study was to investigate the activities of Centella asiatica leaf extract (CAE) against H. pylori both in vitro and in vivo. The minimum inhibitory concentrations (MICs) against 55 clinically isolated strains of H. pylori were tested using an agar dilution method. The MICs of CAE ranged from 0.125 mg/mL to 8 mg/mL, effectiveness in inhibiting H. pylori growth was 2 mg/mL. The anti-H. pylori effects of CAE in vivo were also examined in H. pylori-infected C57BL/6 mice. CAE was orally administrated once daily for 3 weeks at doses of 50 mg/kg and 250 mg/kg. CAE at the 50 mg/kg dose significantly reduced H. pylori colonization in mice gastric mucosa. Our study provides novel insights into the therapeutic effects of CAE against H. pylori infection, and it suggests that CAE may be useful as an alternative therapy.

Therapeutic Potential of Centella asiatica and Its Triterpenes: A Review

“…The mechanisms involved were as follows: the reduction of related inflammation factors (IL−1β ↓, IL−2 ↓, IL−6 ↓, IL−10 ↓, IL−12 ↓, and TNF−α ↓) and the increase in the level of antioxidant stress factors (SOD ↑, CAT ↑, and GPx ↑). Furthermore, evidence showed that C. asiatica could also reduce MDA and COX-2 levels, thereby ameliorating gastric mucosal damage ( Choi et al, 2016 ; Zheng et al, 2016a ; Zheng et al, 2016b ; Intararuchikul et al, 2019 ; Wannasarit et al, 2019 ). The pharmacological effect of asiatic acid is mainly reflected in the improvement in liver fibrosis and acute pancreatitis.…”

Comparative Studies on Different Extraction Methods of Centella asiatica and Extracts Bioactive Compounds Effects on Antimicrobial Activities

“…A study by Zheng et al [ 82 ] using an extract of C. asiatica obtained by the maceration method found that at 2 mg/mL, it inhibited the growth of Helicobacter pylori by the agar well diffusion method; the MIC against the strains tested ranged from 0.125 to 8 mg/mL. Methanol extract obtained by the maceration method showed a broad spectrum of antimicrobial activity using the agar diffusion method against both Gram-positive and -negative bacteria, with the zones of inhibition ranging from 9 to 29 mm and the MIC ranging from 1.25 to >10 mg/mL [ 76 ].…”

“…Figure 10 shows the effectiveness in vivo of C. asiatica extracts obtained using maceration in reducing H. pylori gastric mucosal colonization in a C57BL/6 mouse model. The optimum concentration was 50 mg/kg after oral administration once daily for three weeks [ 82 ].…”

“…* p < 0.05 compared with the control. Figure and caption reused from Zheng et al [ 82 ]. Used under the Creative Commons License ( (accessed on 26 February 2021)).…”

&lt;i&gt;In Vivo&lt;/i&gt; Study of Healing Effects of Sida acuta Leaf Extracts on &lt;i&gt;Helicobacter pylori&lt;/i&gt; Induced Ulceration in Mice

“…The extracts inhibitory activities against H. pylori was also confirmed by the H pylori count in gastric biopsy culture, where the decrease in bacterial population was observed from samples treated with both extracts (cold water and ethanol) and clarithromycin. Several studies have also reported that extracts from several other plants, such as Allium sativum [15], Centella asiatica leaf [16], Aframomum pruinosum seeds [17], Calophyllum brasiliense Camb. [34], turmeric [35] and garlic powder and oil [36] have activities against H. pylori.…”