In vitro and in vivo interactions between Trichoderma viride and Fusarium circinatum

Abstract

Fusarium circinatum, a fungus that causes pitch canker disease, has been present in Europe since at least 2003, when it was detected in northern Spain and found to be producing severe damage in tree nurseries and pine plantations. In this study, we tested a method of biological control of the disease with Trichoderma viride, a fungal species successfully used against many other pathogens. In vitro and in vivo assays were carried out to test the efficacy of this antagonist in controlling F. circinatum. The T. viride isolate exerted a significant effect on the growth of F. circinatum in the in vitro assay, reducing the length of the pathogen colony by half. However, although we tested three different concentrations of the T. viride spore solution, no clear conclusions were obtained with regard to the effects on the Pinus radiata seedlings. To our knowledge, this is the first study carried out with the aim of using Trichoderma spp. to control pitch canker disease.

Sydowia polyspora Dominates Fungal Communities Carried by Two Tomicus Species in Pine Plantations Threatened by Fusarium circinatum

“…Specifically, F. sporotrichioides is considered as an opportunistic pathogen while F. lateritium is not only considered as nonpathogenic but also as a possible antagonist against F. circinatum when F. lateritium performs an earlier colonization [49]. In this way, plot A showed evident symptoms of PPCD, and the presence of the pathogen in this area has been proved in previous studies [63,64]. In spite of this, F. circinatum was not present in collected insects either from this infected plot or from plot B.…”

In vitro antagonistic activity of a root endophytic fungus towards plant pathogenic fungi

“…Inhibitory effect of endophytes was studied on root rot pathogen, Heterobasidion parviporum [15]. In vitro and in vivo assays were carried out to test the efficacy of T. viride antagonist in controlling Fusarium circinatum causing pitch canker in Pinus [16].…”

Antagonismo in vitro de hongos endófitos para su uso en el biocontrol de enfermedades forestales

“…Los ensayos se llevaron a cabo en placas Petri de 90 mm de diámetro, utilizando medio de cultivo PDAs (3.90% patata-dextrosa-agar, suplementado con 0.50 mg/l de estreptomicina sulfato como agente antibiótico) sellado con papel Parafilm®. Se cultivaron los organismos de forma aislada y se incubaron las placas a 25º C en la oscuridad durante una semana (Martínez-Álvarez et al, 2012).…”

“…De acuerdo a los trabajos publicados por Martínez-Álvarez et al, (2012Martínez-Álvarez et al, ( , 2016 las especies de Trichoderma spp., frente a F. circinatum. Demostró un elevado efecto de control inhibitorio por producción de micotoxinas de carácter antifúngicas combinadas con su capacidad de micoparasitar al huésped (Rolando et al, 2013).…”

“…Debe prestarse especial atención a la forma y tiempos de inoculación de los endófitos (Jankowiak, 2006) para obtener el máximo de su potencial colonizador y protector-estimulante (Ruppel et al, 1983). Es recomendable la producción de plantas con semillas y sustrato estéril en el que los endófitos sean inoculados al suelo al momento de la siembra de la semilla (Martínez-Álvarez et al, 2012). En el caso de utilizar plantas provenientes de vivero se recomienda la misma vía de inoculación del endófito con al menos 15 a 42 días de antelación a la inoculación del patógeno respectivo (Aleandri et al, 2015).…”